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  • Book
  • © 2013

Fluorescent Methods to Study Biological Membranes

  • Written by experts in the field
  • Richly illustrated
  • An invaluable source of information for beginners in the field and advanced researchers
  • Includes supplementary material: sn.pub/extras

Part of the book series: Springer Series on Fluorescence (SS FLUOR, volume 13)

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Table of contents (18 chapters)

  1. Front Matter

    Pages i-xiii
  2. Application of NBD-Labeled Lipids in Membrane and Cell Biology

    • Sourav Haldar, Amitabha Chattopadhyay
    Pages 37-50
  3. FRET Analysis of Protein-Lipid Interactions

    • Galyna Gorbenko, Paavo K. J. Kinnunen
    Pages 115-140
  4. Hydration and Mobility in Lipid Bilayers Probed by Time-Dependent Fluorescence Shift

    • Sarka Pokorna, Agnieszka Olżyńska, Piotr Jurkiewicz, Martin Hof
    Pages 141-159
  5. Visual Discrimination of Membrane Domains in Live Cells by Widefield Microscopy

    • Claire E. Butler, Guy Wheeler, Jeremy Graham, Kevin M. Tyler
    Pages 163-184
  6. Quantitative Fluorescence Studies of Intracellular Sterol Transport and Distribution

    • Daniel Wüstner, Frederik W. Lund, Lukasz M. Solanko
    Pages 185-213
  7. Studying Membrane Properties Using Fluorescence Lifetime Imaging Microscopy (FLIM)

    • Martin T. Stöckl, Ranieri Bizzarri, Vinod Subramaniam
    Pages 215-240
  8. Fluorescence Correlation Spectroscopy to Study Membrane Organization and Interactions

    • Monika Zelman-Femiak, Yamunadevi Subburaj, Ana J. García-Sáez
    Pages 241-269
  9. Deciphering Cell Membrane Organization Based on Lateral Diffusion Measurements by Fluorescence Correlation Spectroscopy at Different Length Scales

    • Vincent Rouger, Cyrille Billaudeau, Tomasz Trombik, Sébastien Mailfert, Yannick Hamon, Hai-Tao He et al.
    Pages 271-289
  10. STED-FCS Nanoscopy of Membrane Dynamics

    • Christian Eggeling
    Pages 291-309
  11. Imaging Molecular Order in Cell Membranes by Polarization-Resolved Fluorescence Microscopy

    • Sophie Brasselet, Patrick Ferrand, Alla Kress, Xiao Wang, Hubert Ranchon, Alicja Gasecka
    Pages 311-337
  12. Near-Field Optical Nanoscopy of Biological Membranes

    • Thomas S. van Zanten, Carlo Manzo, Maria F. Garcia-Parajo
    Pages 339-363
  13. New Fluorescent Strategies Shine Light on the Evolving Concept of GPCR Oligomerization

    • Martin Cottet, Orestis Faklaris, Eric Trinquet, Jean-Philippe Pin, Thierry Durroux
    Pages 389-415
  14. TNF Receptor Membrane Dynamics Studied with Fluorescence Microscopy and Spectroscopy

    • Felix Neugart, Darius Widera, Barbara Kaltschmidt, Christian Kaltschmidt, Mike Heilemann
    Pages 439-455
  15. Back Matter

    Pages 479-485

About this book

Biological membranes play a central role in cell structure, shape and functions. However, investigating the membrane bilayer has proved to be difficult due to its highly dynamic and anisotropic structure, which generates steep gradients at the nanometer scale. Due to the decisive impact of recently developed fluorescence-based techniques, tremendous advances have been made in the last few years in our understanding of membrane characteristics and functions. In this context, the present book illustrates some of these major advances by collecting review articles written by highly respected experts. The book is organized in three parts, the first of which deals with membrane probes and model membranes. The second part describes the use of advanced quantitative and high-resolution techniques to explore the properties of biological membranes, illustrating the key progress made regarding membrane organization, dynamics and interactions. The third part is focused on the investigation of membrane proteins using the same techniques, and notably on the membrane receptors that play a central role in signaling pathways and therapeutic strategies. All chapters provide comprehensive information on membranes and their exploration for beginners in the field and advanced researchers alike.

Reviews

From the reviews:

“The book covers all major applications of fluorescence to membrane studies. … The book is addressed to scientists and PhD students using fluorescence methods to study biological membranes and membrane proteins. This is a highly interdisciplinary field, and the book will therefore be of interest to biophysicists, physical chemists, and cellular biologists … . broad overview of different techniques provided by the book is also a good starting point for newcomers to the field. … a valuable resource for everyone studying membranes and membrane proteins.” (Lorenzo Stella, Analytical and Bioanalytical Chemistry, May, 2013)

Editors and Affiliations

  • Faculté de Pharmacie, Laboratoire de Biophotonique, Université de Strasbourg, Illkirch, France

    Yves Mély

  • Faculté de Pharmacie, Laboratoire de Biophotonique et, Université de Strasbourg, Illkirch, France

    Guy Duportail

Bibliographic Information

Buy it now

Buying options

eBook USD 259.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access