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  • Book
  • © 2000

Electrotransformation of Bacteria

  • Protocols on the transformation of about 40 bacterial strains of biotechnolgical or medical relevance
  • Easy-to-follow step-by-step instructions for immediate use at the lab bench
  • Each experiment shows the uniform clear structure and presentation of a Springer Lab Manual

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (33 chapters)

  1. Front Matter

    Pages I-XI
  2. Introduction

    1. Front Matter

      Pages 1-1
    2. General Principles of Bacteria Electrotransformation: Key Steps

      • Natalie Eynard, Justin Teissié
      Pages 3-22
    3. Electrotransformation of Escherichia coli

      • Natalie Eynard, Justin Teissié
      Pages 35-41
    4. Transformation of Bacillus subtilis PB1424 by Electroporation

      • Patrizia Brigidi, Maddalena Rossi, Diego Matteuzzi
      Pages 42-46
  3. Biotechnology and Food Technology

    1. Front Matter

      Pages 47-47
    2. Clostridium in Biotechnology and Food Technology

      • Hans P. Blaschek, Joseph Formanek, C. K. Chen
      Pages 49-55
    3. Electrotransformation of Lactococcus lactis

      • Pascal Le Bourgeois, Philippe Langella, Paul Ritzenthaler
      Pages 56-65
    4. Electroporation of bifidobacteria

      • Maddalena Rossi, Patrizia Brigidi, Diego Matteuzzi
      Pages 72-77
    5. Electrotransformation of Listeria species

      • Janet E. Alexander
      Pages 78-87
    6. Electrotransformation of Sphingomonas paucimobilis

      • Isabel Sá-Correia, Arsénio M. Fialho
      Pages 108-118
  4. Medical and Veterinary Applications

    1. Front Matter

      Pages 125-125
    2. Electrotransformation of Yersinia ruckeri

      • Juan M. Cutrín, Alicia E. Toranzo, Juan L. Barja
      Pages 127-133
    3. Electrotransformation of Enterococci

      • Albrecht Muscholl-Silberhorn, Reinhard Wirth
      Pages 134-139

About this book

Creating bacteria with modified genetic properties allows the specific investigation of these microorganisms. Electrotransformation is a highly efficient and easy to apply technique to introduce genetic material into bacterial cells. A strong electric field is used for this purpose.
In the present manual, protocols for the transformation of about 40 strains of bacteria are described. Emphasis is placed on the individual critical procedural steps, since the practical details mainly depend on the bacterial strain under investigation. This presentation together with the theoretical introductionary chapters, allows the user to modify and adapt each protocol to his/her own experiments. Bacterial strains with relevance in the food industry, biotechnology, medical and veterinary fields, agroindustry and environmental sciences are covered.

Editors and Affiliations

  • Institut de Pharmacologie et Biologie Structurale, CNRS UPR 9062, Toulouse cédex, France

    Natalie Eynard, Justin Teissié

Bibliographic Information

  • Book Title: Electrotransformation of Bacteria

  • Editors: Natalie Eynard, Justin Teissié

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-662-04305-9

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 2000

  • Softcover ISBN: 978-3-540-66680-6Published: 28 June 2000

  • eBook ISBN: 978-3-662-04305-9Published: 29 June 2013

  • Edition Number: 1

  • Number of Pages: XI, 292

  • Number of Illustrations: 8 b/w illustrations

  • Topics: Biotechnology, Microbiology, Molecular Medicine, Cell Biology

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access