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Micro and Nano Technologies in Bioanalysis

Methods and Protocols

  • Book
  • © 2009

Overview

Editors:
  1. Robert S. Foote
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  2. James Weifu Lee
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  • Provides current information on developments in nanotechnologies and their use in biomolecular delivery, gene therapy and expression control
  • Considers the applications of microfluidics and nanopores in the separation, manipulation, detection, and analysis of biomolecules
  • Presents emerging technologies, including applications of quantum dots and molecular fluorescence in the tracking and imaging of biomolecules
  • Contains chapters contributed by nearly 100 authors worldwide, many of whom are ranked among the world’s most prominent scientists in their fields
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 544)

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Table of contents (41 protocols)

  1. Front Matter

    Pages i-xix
    Download chapter PDF

  2. Applications of microfluidics and nanopores in separation, detection, manipulation, and analysis of biomolecules

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. HPLC-Chip/MS Technology in Proteomic Profiling

      • Martin Vollmer, Tom van de Goor
      Pages 3-15

    3. Nanofluidic Channel Fabrication and Manipulation of DNA Molecules

      • Kai-Ge Wang, Hanben Niu
      Pages 17-27

    4. A Single-Molecule Barcoding System using Nanoslits for DNA Analysis

      • Kyubong Jo, Timothy M. Schramm, David C. Schwartz
      Pages 29-42

    5. Microfluidic Devices with Photodefinable Pseudo-valves for Protein Separation

      • Z. Hugh Fan
      Pages 43-52

    6. Microfluidic Chips Designed for Measuring Biomolecules Through a Microbead-Based Quantum Dot Fluorescence Assay

      • Kwang-Seok Yun, Dohoon Lee, Hak-Sung Kim, Euisik Yoon
      Pages 53-67

    7. DNA Focusing Using Microfabricated Electrode Arrays

      • Faisal A. Shaikh, Victor M. Ugaz
      Pages 69-79

    8. Solid-State Nanopore for Detecting Individual Biopolymers

      • Jiali Li, Jene A. Golovchenko
      Pages 81-93

    9. Inserting and Manipulating DNA in a Nanopore with Optical Tweezers

      • U. F. Keyser, J. van der Does, C. Dekker, N. H. Dekker
      Pages 95-112

    10. Forming an α-Hemolysin Nanopore for Single-Molecule Analysis

      • Nahid N. Jetha, Matthew Wiggin, Andre Marziali
      Pages 113-127

    11. Nanopore Force Spectroscopy on DNA Duplexes

      • Nahid N. Jetha, Matthew Wiggin, Andre Marziali
      Pages 129-150

  3. Technologies of physical science and chemistry in detection and analysis of biomolecules

    1. Front Matter

      Pages 152-152
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    2. Quantitative Chemical Analysis of Single Cells

      • Michael L. Heien, Andrew G. Ewing
      Pages 153-162

    3. Trapping and Detection of Single Molecules in Water

      • M. Willander, K. Risveden, B. Danielsson, O. Nur
      Pages 163-186

    4. ZnO Nanorods as an Intracellular Sensor for pH Measurements

      • M. Willander, Safaa Al-Hilli
      Pages 187-200

    5. Analysis of Biomolecules Using Surface Plasmons

      • M. Willander, Safaa Al-Hilli
      Pages 201-229

    6. Use of Residual Dipolar Couplings in Structural Analysis of Protein-Ligand Complexes by Solution NMR Spectroscopy

      • Nitin U. Jain
      Pages 231-252

    7. Raman-Assisted X-Ray Crystallography for the Analysis of Biomolecules

      • Dominique Bourgeois, Gergely Katona, Eve de Rosny, Philippe Carpentier
      Pages 253-267

    8. Methods and Software for Diffuse X-Ray Scattering from Protein Crystals

      • Michael E. Wall
      Pages 269-279
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Keywords

About this book

In recent years, large-scale advances in technology have led to greater understanding of the world on a much tinier scale: the biomolecular level. In Micro and Nano Technologies in Bioanalysis: Methods and Protocols, expert researchers from across the globe explore the technology which makes this analysis possible, investigating the worlds of microfluidics and nanotechnologies, and examining physical science techniques for the separation, detection, manipulation, and analysis of biomolecules. This volume contains innovative protocols on the application of microfluidics and the utilization of physical science-related technologies that will prove to be invaluable in the field of molecular biology. Chapters contain cutting edge applications of emerging nanotechnologies, including quantum dots and molecular fluorescence for the imaging and tracking of biomolecules. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and groundbreaking, Micro and Nano Technologies in Bioanalysis: Methods and Protocols is a necessary tool for cellular biologists, biochemists, microbiologists, geneticists and medical researchers alike.

 

Reviews

From the reviews: “This book provides a comprehensive overview of different modern methods with acknowledged practical interest. … this book can be considered as unique and highly valuable. … This book can be recommended for scientists, technicians and students in the fields of molecular and cell biology, biochemistry, microbiology, genetics, medical research and analytical chemistry. … This book is an excellent source for interested users to learn about recent developments of emerging microtechnologies and nanotechnologies for bioanalysis.” (Rosario Pereiro, Analytical and Bioanalytical Chemistry, November, 2009) “This book describes specific micro and nano techniques used in analyzing functions in both individual cells and animal models. It includes details and tips for using these methods. … Written for scientists at many different levels, the book includes specific step-by-step instructions in each chapter that would be useful for senior scientists as well as bench technical assistants. … will be a valuable resource for biomedical scientists.” (Rebecca T. Horvat, Doody’s Review Service, February, 2010)

Bibliographic Information

  • Book Title: Micro and Nano Technologies in Bioanalysis

  • Book Subtitle: Methods and Protocols

  • Editors: Robert S. Foote, James Weifu Lee

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-483-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-934115-40-4Published: 21 July 2009

  • Softcover ISBN: 978-1-61779-487-2Published: 01 December 2011

  • eBook ISBN: 978-1-59745-483-4Published: 02 June 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XX, 668

  • Number of Illustrations: 107 b/w illustrations, 36 illustrations in colour

  • Topics: Biotechnology, Nanotechnology and Microengineering, Materials Science, general, Nanotechnology, Biomaterials

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