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Chemistry - Biotechnology | Animal Cell Biotechnology - Methods and Protocols

Animal Cell Biotechnology

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1104

Pörtner, Ralf (Ed.)

3rd ed. 2014, XIV, 498 p. 83 illus., 28 illus. in color.

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Animal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially high-throughput synchronization, insect cell lines, transient gene and protein expression, DNA Profiling and Characterisation. Part II addresses tools for process and medium optimization as well as microcarrier technology while Part III covers monitoring of cell growth, viability and apoptosis, metabolic flux estimation, quenching methods as well as NMR-based techniques. Part IV details cultivation techniques, and Part V describes special applications, including vaccine production, baculovirus protein expression, chromatographic techniques for downstream as well as membrane techniques for virus separation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Animal Cell Biotechnology: Methods and Protocols, Third Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.

Content Level » Professional/practitioner

Keywords » DNA profiling - animal cell lines - cell culture - gene expression - protein expression

Related subjects » Biotechnology - Cell Biology

Table of contents 

Part I. Cell Line Development and Selection


1. High-Throughput Synchronization of Mammalian Cell Cultures by Spiral Microfluidic Sorter

            Wong Cheng Lee, Ali Asgar. S. Bhagat, and Chwee Teck Lim


2. Insect Cell Line Development Using Flp-Mediated Cassette Exchange Technology

João Vidigal, Fabiana Fernandes, Ana S Coroadinha, Ana P Teixeira, and Paula M. Alves


3. Scalable Transient Gene Expression in Adherent Mammalian Cells Using Polyethylenimine

Lukas Fliedl and Christian Kaisermayer


4. Scalable Transient Protein Expression

Henry C. Chiou, Sanjay Vasu, Chao Yan Liu, Isabel Cisneros, Meredith B. Jones, and Jon Zmuda


5. DNA Profiling and Characterisation of Animal Cell Lines

Glyn N. Stacey, Ed Byrne, and J. Ross Hawkins



Part II. Techniques for Process Development


6. Tools for High-Throughput Process and Medium Optimization

Martin Jordan and Matthieu Stettler


7. Designing Media for Animal Cell Culture – CHO Cells, The Industrial Standard 

Karlheinz Landauer


8. Feed Optimization in Fed-Batch Culture

Ana Rita Costa, Maria Elisa Rodrigues, Mariana Henriques, Rosário Oliveira, and Joana Azeredo


9. Screening and Optimization of Chemically-Defined Media and Feeds with Integrated and Statistical Approaches

Zhihua Xiao, Michelle Sabourin, Graziella Piras, and Stephen Gorfien


10. Evaluation of Solid and Porous Microcarriers for Cell Growth and Production of Recombinant Proteins        

Maria Elisa Rodrigues, Ana Rita Costa, Mariana Henriques, Joana Azeredo, and Rosário Oliveira


11. Microbioreactors and Scale-Down Models: Growth of CHO Cells Using the Pall Micro24 MicroReactor System

Steve R.C. Warr



Part III. Cell Characterisation and Analysis


12. Monitoring Cell Growth, Viability and Apoptosis

Michael Butler, Maureen Spearman, and Katrin Braasch


13. Metabolic Flux Estimation in Mammalian Cell Cultures

Chetan T. Goudar, Richard K. Biener, James M. Piret, and Konstantin B. Konstantinov


14. Quenching Methods for the Analysis of Intracellular Metabolites

Judith Wahrheit and Elmar Heinzle


15. NMR Methods for Metabolomics of Mammalian Cell Culture Bioreactors

Nelly Aranibar and Michael D. Reily


16. 1H-NMR Protocol for Exometabolome Analysis of Cultured Mammalian Cells

Tiago M. Duarte, Nuno Carinhas, Ana Carina Silva, Paula M. Alves, and Ana P. Teixeira



Part IV. Cultivation Techniques


17. Microfluidic Perfusion Culture

Koji Hattori, Shinji Sugiura, and Toshiyuki Kanamori


18. Disposable Bioreactors for Inoculum Production and Protein Expression          Regine Eibl, Christian Löffelholz, and Dieter Eibl


19. Configuration of Bioreactors

Dirk E. Martens, E. J. van den End, and Mathieu Streefland


20. Process Analytical Technology (PAT) in Insect and Mammalian Cell Culture Processes – Dielectric Spectroscopy and Focused Beam Reflectance Measurement (FBRM)

Damir Druzinec, Katja Weiss, Christiane Elseberg, Denise Salzig, Matthias Kraume, Ralf Pörtner, and Peter Czermak


21. Cell Assessment by Atline Microscopy

Alexander Babitzky, Patrick Lindner, and Thomas Scheper


22. Seed Train Optimization for Cell Culture

Björn Frahm



Part V. Special Applications


23. Vaccine Production - Upstream Processing with Adherent or Suspension Cell Lines   

Yvonne Genzel, Jana Rödig, Erdmann Rapp, and Udo Reichl


24. Efficient Processes for Protein Expression Using Recombinant Baculovirus Particles

Heike Cremer, Ingrid Bechtold, Marion Mahnke, and René Assenberg


25. Chromatographic Techniques in the Downstream Processing of Proteins in Biotechnology

            Ruth Freitag


26. Virus Separation Using Membranes        

Tanja A. Grein, Ronald Michalsky, and Peter Czermak

Distribution rights 

Distribution rights for India: Scientific International, New Delhi, India

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