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Chemistry - Biotechnology | Synthetic Biology

Synthetic Biology

Series: Methods in Molecular Biology, Vol. 1073

Polizzi, Karen M., Kontoravdi, Cleo (Eds.)

2013, X, 226 p. 80 illus., 48 illus. in color.

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  • Reviews the latests developments in molecular biology techniqueds
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

 In Synthetic Biology, expert researchers in the field provide the latest developments in molecular biology techniques used in Synthetic Biology.  Focusing on computational tools that will aid in systematising the design and construction of parts and systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Synthetic Biology seeks to aid scientists in the further study of developing new biological components and systems.

Content Level » Professional/practitioner

Keywords » Synthetic biology - behaviour - biological components - computational tools - constructing synthetic biological networks - embed novel functions into existing ones - engineering - molecular biology techniques - standardization

Related subjects » Biotechnology

Table of contents 

Part I: Introduction

1. What is Synthetic Biology?

Karen M Polizzi


Part II: DNA Assembly

2. Gene Synthesis Method Based on Overlap Extension PCR and DNAWorks Program

Gang Li, Bing-Xue Dong, Yu-Huan Liu, Chang-Jie Li, and Li-Ping Zhang


3. BioBrick Assembly Using the In-Fusion PCR Cloning Kit

Sean C. Sleight and Herbert M. Sauro


4. Overlap Extension PCR Cloning

Anton Bryksin and Ichiro Matsumura


5. One-Step Isothermal Assembly of DNA Fragments

Rui TL Rodrigues and Travis S Bayer



PartIII: Part, Pathway, and Genome Engineering

6. Creation and Characterization of Component Libraries for Synthetic Biology

Tim Weenink and Tom Ellis


7. In vivo and in vitro Characterisation of s70 Constitutive Promoter BioParts by Real-time PCR and Fluorescent Measurements

James Chappell and Paul Freemont


8. In Vivo Screening of Artificial Small RNAs for Silencing Endogenous Genes in Escherichia coli

Vandana Sharma and Yohei Yokobayashi


9. Construction and Engineering of  Large Biochemical Pathways via DNA Assembler

Zengyi Shao and Huimin Zhao

10. Assembly of Multi-gene Pathways and Combinatorial Pathway Libraries through ePathBrick Vectors

Peng Xu and Mattheos A.G. Koffas


11. Tandem Recombineering by SLIC Cloning and Cre-LoxP Fusion to Generate Multigene Expression Constructs for Protein Complex Research

Matthias Haffke, Cristina Viola, Yan Nie, and Imre Berger


12. Combinatorial DNA Assembly using Golden Gate Cloning

Carola Engler and Sylvestre Marillonnet


13. Construction of Synthetic Gene Circuits in the Escherichia coli Genome

Bei-Wen Ying, Yuya Akeno, and Tetsuya Yomo

 14. Shuffling of DNA Cassettes in a Synthetic Integrin

David Bikard and Didier Mazel



Part IV: Computational Tools for Modelling Biological Systems

15. Systematic Methodology for the Development of Mathematical Models for Biological Processes

Cleo Kontoravdi


16. SOBOLHDMR: A General Purpose Modelling Software

Dr Sergei Kucherenko

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