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Synthetic Biology

  • Book
  • © 2013

Overview

Editors:
  1. Karen M. Polizzi

    1. Division of Molecular Biosciences, Imperial College London, London, United Kingdom

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    You can also search for this editor in PubMed   Google Scholar

  2. Cleo Kontoravdi

    1. Department of Chemical Engineering, Imperial College London, London, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Reviews the latests developments in molecular biology techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1073)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
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  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. What Is Synthetic Biology?

      • Karen M. Polizzi
      Pages 3-6

  3. DNA Assembly

    1. Front Matter

      Pages 7-7
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    2. Gene Synthesis Method Based on Overlap Extension PCR and DNAWorks Program

      • Gang Li, Bing-Xue Dong, Yu-Huan Liu, Chang-Jie Li, Li-Ping Zhang
      Pages 9-17

    3. BioBrickâ„¢ Assembly Using the In-Fusion PCR Cloning Kit

      • Sean C. Sleight, Herbert M. Sauro
      Pages 19-30

    4. Overlap Extension PCR Cloning

      • Anton Bryksin, Ichiro Matsumura
      Pages 31-42

    5. One-Step Isothermal Assembly of DNA Fragments

      • Rui T. L. Rodrigues, Travis S. Bayer
      Pages 43-47

  4. Part, Pathway, and Genome Engineering

    1. Front Matter

      Pages 49-49
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    2. Creation and Characterization of Component Libraries for Synthetic Biology

      • Tim Weenink, Tom Ellis
      Pages 51-60

    3. In Vivo and In Vitro Characterization of σ 70 Constitutive Promoters by Real-Time PCR and Fluorescent Measurements

      • James Chappell, Paul Freemont
      Pages 61-74

    4. In Vivo Screening of Artificial Small RNAs for Silencing Endogenous Genes in Escherichia coli

      • Vandana Sharma, Yohei Yokobayashi
      Pages 75-84

    5. Construction and Engineering of Large Biochemical Pathways via DNA Assembler

      • Zengyi Shao, Huimin Zhao
      Pages 85-106

    6. Assembly of Multi-gene Pathways and Combinatorial Pathway Libraries Through ePathBrick Vectors

      • Peng Xu, Mattheos A. G. Koffas
      Pages 107-129

    7. Tandem Recombineering by SLIC Cloning and Cre-LoxP Fusion to Generate Multigene Expression Constructs for Protein Complex Research

      • Matthias Haffke, Cristina Viola, Yan Nie, Imre Berger
      Pages 131-140

    8. Combinatorial DNA Assembly Using Golden Gate Cloning

      • Carola Engler, Sylvestre Marillonnet
      Pages 141-156

    9. Construction of Synthetic Gene Circuits in the Escherichia coli Genome

      • Bei-Wen Ying, Yuya Akeno, Tetsuya Yomo
      Pages 157-168

    10. Shuffling of DNA Cassettes in a Synthetic Integron

      • David Bikard, Didier Mazel
      Pages 169-174

  5. Computational Tools for Modelling Biological Systems

    1. Front Matter

      Pages 175-175
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    2. Systematic Methodology for the Development of Mathematical Models for Biological Processes

      • Cleo Kontoravdi
      Pages 177-190
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Keywords

About this book

 In Synthetic Biology, expert researchers in the field provide the latest developments in molecular biology techniques used in Synthetic Biology.  Focusing on computational tools that will aid in systematising the design and construction of parts and systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Synthetic Biology seeks to aid scientists in the further study of developing new biological components and systems.

Editors and Affiliations

  • Division of Molecular Biosciences, Imperial College London, London, United Kingdom

    Karen M. Polizzi

  • Department of Chemical Engineering, Imperial College London, London, United Kingdom

    Cleo Kontoravdi

Bibliographic Information

  • Book Title: Synthetic Biology

  • Editors: Karen M. Polizzi, Cleo Kontoravdi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-625-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2013

  • Hardcover ISBN: 978-1-62703-624-5Published: 01 September 2013

  • Softcover ISBN: 978-1-4939-6286-0Published: 23 August 2016

  • eBook ISBN: 978-1-62703-625-2Published: 31 August 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 226

  • Number of Illustrations: 32 b/w illustrations, 48 illustrations in colour

  • Topics: Biotechnology, Biochemical Engineering

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