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Chemistry - Biotechnology | Oxidative Stress and Nanotechnology - Methods and Protocols

Oxidative Stress and Nanotechnology

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1028

Armstrong, Donald, Bharali, Dhruba J (Eds.)

2013, XI, 294 p. 54 illus., 32 illus. in color.

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  • Provides a comprehensive handbook to oxidative stress from the nanotechnology standpoint
  • Contains detailed, step-by-step protocols
  • Features notes on troubleshooting and avoiding known pitfalls

The emergence of nanotechnology has had a profound effect on almost every aspect of the 21st century’s daily life. It has had a revolutionary impact from stain-resistant clothing and cosmetics to environmental issues , including energy and medicine and even aerospace engineering.  In Oxidative Stress and Nanotechnology: Methods and Protocols, expert researchers in the field detail various aspects of nanotechnology from the oxidative stress point of view. Focusing on synthesis of different antioxidant nanoparticles and antioxidant-loaded nanoparticles, as well as their in vitro/ in vivo mechanisms of action along with their clinical relevance. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Oxidative Stress and Nanotechnology: Methods and Protocols seeks to aid scientists in the further study of techniques for nanotechnology in pathophysiology and protection from the oxidative stress point of view.

Content Level » Professional/practitioner

Keywords » antioxidant - antioxidant nanoparticles - antioxidant-loaded nanoparticles - diseases - inorganic and polymeric nanoparticles - nanomedicine - nanoparticle - nanotechnology - oxidative stress

Related subjects » Biotechnology - Nanotechnology

Table of contents 

Part I: Reviews

1. Nanoscale Biosensor for Detection of Reactive Oxygen Species

Tarl W. Prow, Daniel Sundh, and Gerard A. Lutty


2. Nanoparticles: Toxicity, Radicals, Electron Transfer and Antioxidants

Peter Kovacic and Ratnasamy Somanathan


3. Encapsulation of Anti-Oxidants in Gastrointestinal Resistant Nanoparticulate Carriers

Eliana B. Souto, Patrícia Severino, Rafael Basso, and Maria Helena A. Santana


4. Toxicity of Novel Nanosized Formulations Used in Medicine

Afaf El-Ansary , Sooad Al-Daihan, Abir Ben Bacha , and Malak Kotb


5. Fullerenol Nanoparticles: Toxicity and Antioxidant Activity

Rade Injac, Matevz Prijatelj, and Borut Strukelj


6. Hydrogen Peroxide (H2O2) Detection with Nanoprobes for Biological Applications: A Mini-Review

Gwangseong Kim, Yong-Eun Koo Lee, and Raoul Kopelman


7. Studying the Oxidative Stress Paradigm in vitro: A theoretical and Practical Perspective

Martin J. D. Clift and Barbara Rothen-Rutishauser


8. Evaluation of the Effect of Acute and Sub-acute Exposure to TiO2 Nanoparticles on Oxidative Stress

Shichang LIU, Zhuo YANG


9. Biomedical Applications of Nano-antioxidant

Geeta Watal, Aparna Watal , Prashant Kumar Rai, Devendra Kumar Rai, Bechan Sharma


Part II: Protocols

10. Lipid Peroxidation Due to In Vitro and In Vivo Exposure of Biological Samples to Nanoparticles

Anca Dinischiotu, Loredana Stanca,  Daniela Gradinaru, Sorina Nicoleta Petrache,  Mihaela Radu, Andreea Iren Serban


11. Testing Nanomaterial Toxicity in Unicellular Eukaryotic Algae and Fish Cell Lines

Alexandra Kroll, Dana Kühnel, and Kristin Schirmer


12. Gold Nanoparticles and Oxidative Stress in the Blue Mussel, Mytilus edulis

Sara Tedesco, Hugh Doyle, Daniela Iacopino, Irene O’Donovan, Sarah Keane, and David Sheehan


13. Oxidative Biomarkers to Assess the Nanoparticles Induced Oxidative Stress

A. Rama Narsimha Reddy, Y. Narsimha Reddy, and Krishna R. Devarakonda


14. Vesicular Antioxidants: Role in Age-related Cerebral Oxidative Injury

Sibani Sarkar, Ardhendu Kumar Mandal, and Nirmalendu Das


15. Methods for Detection of Oxidative Stress and Genotoxicity of Engineered Nanoparticles

Ashutosh Kumar, Vyom Sharma, and Alok Dhawan


16. Size Dependent Study of Pulmonary Responses to Nano-sized Iron and Copper Oxide Nanoparticles

Rajiv Kumar and Dattatri K Nagesha


17. In vitro Techniques to Investigate the Oxidative Effects of Quantum Dots

Ridhima Juneja and Indrajit Roy


18. Hybrid Polymeric Nanoparticles: Potential Candidate for Ophthalmic Delivery

Dhruba J Bharali, Donald Armstrong, and Shaker A Mousa


Part III: Future Directions

19. Relevance of Nanotechnology in Modulating Oxidative Stress:  An Overview

Dhruba J Bharali and Shaker A Mousa


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