Overview
- Provides practical methods and protocols involving tissue-based laser microdissection, ideal for labs around the globe
- Fully updates and reexamines the popular first edition
- Features key notes and expert implementation advice
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 755)
Access this book
Tax calculation will be finalised at checkout
Other ways to access
Table of contents (40 protocols)
-
Front Matter
Keywords
About this book
Laser microdissection techniques have revolutionized the ability of researchers in general, and pathologists in particular, to carry out molecular analysis on specific types of normal and diseased cells and to fully utilize the power of current molecular technologies including PCR, microarrays, and proteomics. In second edition of Laser Capture Microdissection: Methods and Protocols, experts in the field provide the reader with practical advice on how to carry out tissue-based laser microdissection successfully in their own laboratory using the different laser microdissection systems that are available and to apply a wide range of molecular technologies. The individual chapters encompass detailed descriptions of the individual laser based micro-dissection systems. The downstream applications of the laser microdissected tissue described in the book include PCR in its many different forms as well as gene expression analysis including application to microarrays and proteomics. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Â
Authoritative and cutting-edge, Laser Capture Microdissection: Methods and Protocols, Second Edition is an ideal resource for researchers striving to move forward our understanding of normal physiology and pathology.Editors and Affiliations
Bibliographic Information
Book Title: Laser Capture Microdissection
Book Subtitle: Methods and Protocols
Editors: Graeme I. Murray
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-163-5
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2011
Hardcover ISBN: 978-1-61779-162-8Published: 19 July 2011
Softcover ISBN: 978-1-4939-6080-4Published: 23 August 2016
eBook ISBN: 978-1-61779-163-5Published: 19 July 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XVII, 493
Number of Illustrations: 96 b/w illustrations, 1 illustrations in colour
Topics: Biotechnology, Gene Expression