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Chemistry - Biotechnology | Animal Cell Biotechnology - Methods and Protocols

Animal Cell Biotechnology

Methods and Protocols

Series: Methods in Biotechnology, Vol. 24

Pörtner, Ralf (Ed.)

2nd ed. 2007, XV, 512 p.

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  • Chapters written by world-renowned experts
  • Details novel techniques for basic cultivation and complex techniques such as disposable bioreactors, hollow fiber cell culture and fixed bed reactors
  • Provides a state-of-the-art manual for setting up mammalian cell lines for production of biopharmaceuticals
  • Divided into sections to mimic the processes required for different stages of production

The second edition of this book constitutes a comprehensive manual of new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production.

The volume’s five parts reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially transduction techniques, cells for gene therapy and antibody production. Part II addresses basic cultivation techniques, such as microcarrier culture and encapsulation.

Part III covers cell characterization and analysis, including flow cytometric applications, NMR-based techniques, and biochemical and cytometric techniques. Part IV details cultivation techniques, such as disposable bioreactors, hollow fiber cell culture, fixed bed reactors, and configuration of bioreactors. Part V covers downstream techniques such as membrane filtration techniques, while Part VI describes special applications, including retroviral vectors.

This book provides a compendium of techniques for scientists in industr

Content Level » Research

Keywords » DNA - Downstream Processing - Expression - biotechnology - cell lines - gene therapy - genes - mammalian cell lines - metabolism - physiology

Related subjects » Animal Sciences - Biotechnology - Cell Biology - Pharmacology & Toxicology

Table of contents 

Animal Cell Biotechnology - Methods and Protocols Editor: Ralf Pörtner Contents Preface Contributors Part I. Basic techniques for establishing production cell lines 1 Transduction Technologies David A. Fluri and Martin Fussenegger 2 Cells for gene therapy and vector production C. Delenda, M. Chillon, A.-M. Douar and Otto-Wilhelm Merten 3 Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments Alexandra Dorn-Beineke, Stefanie Nittka and Michael Neumaier 4 DNA Fingerprinting and Characterisation of Animal Cell Lines Glyn N. Stacey, Ed Byrne and J. Ross Hawkins Part II. Basic cultivation techniques 5 Microcarrier Cell culture Technology Gerald Blüml 6 Cell Encapsulation Holger Hübner 7 Tools for High-Throughput Medium and Process Optimisation Martin Jordan and Nigel Jenkins Part III. Cell characterisation and analysis 8 Cell counting and viability measurements Michael Butler and Maureen Spearman 9 Monitoring of Growth, Physiology and Productivity of animal cells by Flow Cytometry Silvia Carroll, Mariam Nasiri and Mohamed Al-Rubeai 10 Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors André A. Neves and Kevin M. Brindle 11 Methods for off-line analysis of nutrients and products in mammalian cell culture Heino Büntemeyer 12 Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation Derek Adams, Rashmi Korke, and Wei-Shou Hu 13 Measurement of apoptosis in cell culture Adiba Ishaque1 and Mohamed Al-Rubeai 14 Metabolic Flux Estimation in Mammalian Cell Cultures Chetan T. Goudar, Richard Biener, JamesM. Piret and Konstantin B. Konstantinov Part IV. Special cultivation techniques 15 Disposable bioreactors for inoculum production and protein expression Regine Eibl and Dieter Eibl 16 Hollow Fibre Cell Culture John M. Davis 17 Cultivation of mammalian cells in fixed bed reactors Ralf Pörtner and Oscar B.J. Platas Barradas 18 Configuration of Bioreactors Dirk E. Martens and Evert Jan van den End Part V. Downstream techniques 19 Membrane filtration in animal cell culture Peter Czermak, Dirk Nehring and Ranil Wickramasinghe 20 Chromatographic techniques in the downstream processing of (recombinant) proteins Ruth Freitag Part VI. Special applications 21 Vaccine production - state of the art and future needs in upstream processing Yvonne Genzel and Udo Reichl 22 Retrovirus production and characterization Pedro E. Cruz, Marlene Carmo, Teresa Rodrigues, Paula Alves 23 Insect Cell Cultivation and Generation of Recombinant Baculovirus Particles for Recombinant Protein Production Sabine Geisse Index

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Distribution rights In India: Panima Educational Book Agency, New Delhi, India

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