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Methods of Hybridoma Formation

  • Book
  • © 1987

Overview

Editors:
  1. Arie H. Bartal

    1. Biotherapeutics, Inc., Franklin, USA
      Hybridoma Laboratory, Northern Israel Oncology Center, Rambam Medical Center, Haifa, Israel

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    You can also search for this editor in PubMed   Google Scholar

  2. Yashar Hirshaut

    1. Sloan-Kettering Cancer Center, New York, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Contemporary Biomedicine (CB, volume 7)

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Table of contents (26 chapters)

  1. Front Matter

    Pages i-xxv
    Download chapter PDF

  2. Current Methodologies in Hybridoma Formation

    • Arie H. Bartal, Yashar Hirshaut
    Pages 1-40

  3. Retroviruses and Hybridoma Formation

    • Arie H. Bartal, Yashar Hirshaut
    Pages 41-61

  4. Mechanisms of Cell Fusion and Selection in the Generation of Hybridomas

    • Jerry W. Shay
    Pages 63-75

  5. Chemically Mediated Cell Fusion

    • Robert J. Klebe, Kevin L. Bentley
    Pages 77-96

  6. Electrofusion of Cells

    • U. Zimmermann
    Pages 97-149

  7. Enhancement of Hybridoma Formation

    • Arie H. Bartal, Yashar Hirshaut
    Pages 151-161

  8. Modification of HAT Medium and Hybridoma Formation

    • Arie H. Bartal, Carl Feit, Yashar Hirshaut
    Pages 163-179

  9. Culture Methods for the Selection and Isolation of Stable Antibody-Producing Murine Hybridomas

    • R. Thomas Taggart
    Pages 181-194

  10. Selection of Growth Factors and Myelomas To Enhance Monoclonal Antibody-Producing Hybridoma Formation

    • Janet H. Ransom, Martin V. Haspel
    Pages 195-208

  11. Proliferation and Immune Secretion of B-Cell Hybridomas

    • Regine J. J. M. Westerwoudt
    Pages 209-230

  12. Statistical Assessment of Hybridoma Monoclonality After Subcloning by the Limiting Dilution Technique

    • Hilary A. Coller, Barry S. Coller
    Pages 231-236

  13. In Vitro Immunization for the Generation of Hybridomas Using Serum-Free Medium

    • Frederick J. Darfler
    Pages 237-248

  14. Intrasplenic Immunization for the Production of Monoclonal Antibodies

    • M. Spitz, Lidia Spitz
    Pages 249-255

  15. Use of Heteromyelomas in the Enhancement of Human Monoclonal Antibody Production

    • Nelson N. H. Teng, Marcia Bieber
    Pages 257-271

  16. Human Hybridomas

    • Danuta Kozbor, Carlo M. Croce
    Pages 273-298

  17. Antibody-Secreting Human-Human Hybridomas

    • Lennart Olsson
    Pages 299-316

  18. Interspecific Hybridomas

    • John A. Sogn
    Pages 317-335

  19. Rat-Rat Hybridoma Formation and Rat Monoclonal Antibodies

    • Hervé Bazin
    Pages 337-378

  20. Generation of Mutant Monoclonal Antibodies

    • Gad Spira, Hector L. Aguila, Ellyn Fischberg, Matthew D. Scharff
    Pages 379-397
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Keywords

About this book

Laymen often consider modern laboratory research to be based on an endless array of sophisticated technologies whose complex capabilities are as important to the outcome of any project as the inventiveness and creativity of the scientists who employ them. Scientists at times may share this point of view until they are con­ fronted by unexpected findings that demand new approaches, and they discover that yesterday's "sophisticated tools" are today's "blunt instruments." This experience provides a more sobering view of the current state of our scientific methods. It also serves as an impetus for the further development of technology that prepares us for the next stage of advance. Immunologists were confronted by such a technological crises in the late 1970s when they finally were forced to admit that poly­ clonal antibodies, although quite sensitive reagents, were not spe­ cific enough to answer many of the questions then confronting virologists and tumor biologists. The answer to the need for specific­ ity came with the development of monoclonal antibody technology. In the last ten years there have been considerable advances in monoclonal antibody techniques. Today these reagents are much more versatile than they were initially and can be applied to a broad range of problems. Still, most workers who are using these anti­ bodies are convinced that their potential is far from exhausted, and that at least in some fields we are currently in the early stages of learning how to use them properly.

Editors and Affiliations

  • Biotherapeutics, Inc., Franklin, USA

    Arie H. Bartal

  • Hybridoma Laboratory, Northern Israel Oncology Center, Rambam Medical Center, Haifa, Israel

    Arie H. Bartal

  • Sloan-Kettering Cancer Center, New York, USA

    Yashar Hirshaut

Bibliographic Information

  • Book Title: Methods of Hybridoma Formation

  • Editors: Arie H. Bartal, Yashar Hirshaut

  • Series Title: Contemporary Biomedicine

  • DOI: https://doi.org/10.1007/978-1-4612-4826-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Book Archive

  • Copyright Information: The Humana Press Inc. 1987

  • Hardcover ISBN: 978-0-89603-100-5Published: 03 August 1987

  • Softcover ISBN: 978-1-4612-9179-4Published: 01 October 2011

  • eBook ISBN: 978-1-4612-4826-2Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: 504

  • Topics: Biotechnology, Cell Biology

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