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Sample Preparation in Biological Mass Spectrometry

  • Book
  • © 2011

Overview

Editors:
  1. Alexander R. Ivanov

    1. , Department of Genetics and Complex Disea, Harvard School of Public Health, Harvard, Boston, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Alexander V. Lazarev

    1. , Research and Development, Pressure BioSciences, Inc., South Easton, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • The attention to sample preparation methods in recent years has risen considerably, both in the Academia and in the life science industry, however there are very few books on this topic
  • The book presents examples of successful optimization and validation of sample preparation for biomedical analysis
  • Latest trends in biological analysis fueled by the progress in instrument development and adoption of the latest high resolution and high sensitivity mass spectrometry methods are well covered
  • The book contains comprehensive reviews that would help the reader to acquire the most current experience in certain fields of biological sample preparation
  • Numerous step-by-step protocols help the reader to develop new applications quickly and efficiently and to avoid common problems

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Table of contents (49 chapters)

  1. Front Matter

    Pages i-xxix
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  2. Traditional and Improved Techniques in Sample Preparation for Proteomics

    1. Front Matter

      Pages 1-1
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  3. Traditional And Improved Techniques In Sample Preparation For Proteomics

    1. Gel-Based and Gel-Free Sample Preparation for LC-MS/MS Analysis

      • Xianyin Lai, Frank A. Witzmann
      Pages 3-17

    2. Manipulating the Mass Spectrometric Properties of Peptides through Selective Chemical Modification

      • David Arnott, Peter S. Liu, Patricia Molina, Lilian Phu, Wendy N. Sandoval
      Pages 19-40

    3. Sample Preparation for 2D Electrophoresis and CE-Based Proteomics

      • Judit M. Nagy, Alexandria Lipka, Fiona Pereira, Nicky Marlin, Stuart Hassard
      Pages 41-60

    4. Filtration as a Sample Preparation Technique Prior to Mass Spectrometry: Selecting the Right Filtration Device

      • Vivek Joshi, Elena Chernokalskaya
      Pages 61-75

    5. Pressure-Assisted Lysis of Mammalian Cell Cultures Prior to Proteomic Analysis

      • Emily A. Freeman, Alexander R. Ivanov
      Pages 77-90

    6. Multiplexed Preparation of Biological Samples for Mass Spectrometry Using Gel Electrophoresis

      • Jeremy L. Norris, Alan A. Doucette
      Pages 91-105

  4. Methods for Improved Proteolytic Digestion

    1. Front Matter

      Pages 107-107
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  5. Methods For Improved Proteolytic Digestion

    1. Development of an On-Bead Digestion Procedure for Immunoprecipitated Proteins

      • Matthew J. Berberich, Jeffrey A. Kowalak, Anthony J. Makusky, Brian Martin, Detlef Vullhorst, Andres Buonanno et al.
      Pages 109-124

    2. Ultra-Fast Sample Preparation for High-Throughput Proteomics

      • Daniel Lopez-Ferrer, Kim K. Hixson, Mikhail E. Belov, Richard D. Smith
      Pages 125-139

    3. Exploring the Capabilities of the Protein Identification by Unconventional Sample Preparation Approaches: LC/MALDI/On-Target Digestion Approach and High Pressure-Assisted In-Gel Tryptic Digestion

      • Melkamu Getie-Kebtie, Michail A. Alterman
      Pages 141-155

  6. Methods for Tissue, Cell, and Organelle Preparation and Analysis

    1. Front Matter

      Pages 157-157
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  7. Methods For Tissue, Cell, And Organelle Preparation And Analysis

    1. Sample Preparation of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue for Proteomic Analyses

      • Diem Tran, Mark Daniels, Ben Colson, Dikran Aivazian, Antonio Boccia, Ingrid Joseph et al.
      Pages 159-170

    2. Brain Proteomics: Sample Preparation Techniques for the Analysis of Rat Brain Samples Using Mass Spectrometry

      • Yoshinori Masuo, Misato Hirano, Junko Shibato, Hyung Wook Nam, Isabelle Fournier, Céline Mériaux et al.
      Pages 171-195

    3. Subcellular Fractionation Using Step-Wise Density Extraction for Mass Spectrometry Analysis

      • WenKui Lan, Marc J. Horn, Fumihiko Urano, Andre Kopoyan, Sun W. Tam
      Pages 197-214
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Keywords

About this book

The aim of this book is to provide the researcher with important sample preparation strategies in a wide variety of analyte molecules, specimens, methods, and biological applications requiring mass spectrometric analysis as a detection end-point. In this volume we have compiled the contributions from several laboratories which are employing mass spectrometry for biological analysis. With the latest inventions and introduction of highly sophisticated mass spectrometry equipment sample preparation becomes an extremely important bottleneck of biomedical analysis. We have a goal of giving the reader several successful examples of sample preparation, development and optimization, leading to the success in analytical steps and proper conclusions made at the end of the day. This book is structured as a compilation of contributed chapters ranging from protocols to research articles and reviews. The main philosophy of this volume is that sample preparation methods have to be optimized and validated for every project, for every sample type and for every downstream analytical technique.

Reviews

Anal Bioanal Chem (2012) 404:1331–1332: DOI 10.1007/s00216-012-6260-8
http://www.springerlink.com/content/7r2569j2175k81m2/

Alexander R. Ivanov, Alexander V. Lazarev (Eds): Sample preparation in biological mass spectrometry

by David C. Muddiman

"This book focuses on the burgeoning field of sample preparation as it relates to downstream integration with mass spectrometry. This is an expanding field, because of the diverse array of biological questions that can be addressed by use of mass spectrometry. A critical aspect of the successful outcome of an experiment is the 800-pound gorilla in the room called“sample preparation”. It is widely accepted by the biological mass spectrometry community that sample preparation is a key aspect of effectively learning more about the in-depth biology under investigation."

"Sample Preparation in Biological Mass Spectrometry is a monograph which addresses a critical issue in a very diverse and important field. Several chapters are written by leaders in their field and are comprehensive and educational, and provide detailed procedures. Other chapters are less substantial in their content. Overall, the book has value for the novice getting started, because it provides a balance of background on the subject with some practical methods of analysis."

Editors and Affiliations

  • , Department of Genetics and Complex Disea, Harvard School of Public Health, Harvard, Boston, USA

    Alexander R. Ivanov

  • , Research and Development, Pressure BioSciences, Inc., South Easton, USA

    Alexander V. Lazarev

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