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Shape your heart to front the hour, but dream not that the hour will lost. Alfred Tennyson Locksley Hall Sixty YeO!, After Numerous advances in the life sciences have necessitated the need to develop gentle, quick and economical methods for separation of proteins/enzymes. Affmity-based separations, with their high selectivity, have thus come to be used more frequently. In the beginning, we just had atrmity chromatography. Today we have several strategies that employ atrmity interactions for separa tion and analysis. This book is an attempt to provide a short introduction and illustrative protocols for some important and current affinity-based approaches inthe area ofdownstream processingofenzymes/proteins. Today, people from a variety of disciplines have to purify proteins. It is hoped that this book will provide some quick insight into the various available options and guide even a novice (in the area ofprotein separation) to obtain his/her target protein at the level of purity that he/she desires.
1 Affinity-Based Separation: An Overview.- 2 Affinity Chromatography.- 3 Expanded Bed Affinity Chromatography.- 4 Perfusion Affinity Chromatography for Rapid Antibody Separations.- 5 Affinity-Based Interactions on Disks for Fast Analysis, Isolation and Conversion of Biomolecules.- 6 Sugars as Affinity Ligands.- 7 Macroaffinity Ligands in Bioseparation.- 8 Cellulose-Binding Fusion Proteins.- 9 Two-Phase Affinity Partitioning of Animal Cells: Implications of Multipoint Interactions.- 10 Affinity-Based Reverse Micellar Separations.- 11 Biosensors: An Emerging Tool in Bioanalytical Research -A Kinetic Analysis of the Analyte-Receptor Interactions in Biosensors.- Guide to Solutions.- Guide to Enzymes.- Guide to Protocols.- Troubleshooting Guide.