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Methods for Affinity-Based Separations of Enzymes and Proteins

  • Book
  • © 2002

Overview

Editors:
  1. Munishwar Nath Gupta

    1. Department of Chemistry, Indian Institute of Technology, New Delhi, India

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  • SURVEYS THE VARIOUS AFFINITY-BASED SEPARATION METHODS FOR PROTEINS DETAILED METHODOLOGY GUIDES THAT CAN BE USED DIRECTLY TO SET UP AND PERFORM EXPERIMENTS COVERS BOTH TRADITIONAL
  • AS NOVEL AND EMERGING APPROACHES IN BIOSEPARATION
  • FOCUS ON NOVEL, FAST, EFFICIENT AND ECONOMICAL METHODS OF PROTEIN SEPARATION APPROACHES FOR ANALYTICAL LEVEL AND PREPARATIVE SCALE SEPARATIONS EXTENSIVE TROUBLESHOOTING GUIDE

Part of the book series: Methods and Tools in Biosciences and Medicine (MTBM)

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Table of contents (11 chapters)

  1. Front Matter

    Pages I-X
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  2. Affinity-Based Separation: An Overview

    • Munishwar N. Gupta, Ipsita Roy
    Pages 1-15

  3. Affinity Chromatography

    • Nikolaos E. Labrou
    Pages 16-28

  4. Expanded Bed Affinity Chromatography

    • Arvind Lali
    Pages 29-64

  5. Perfusion Affinity Chromatography for Rapid Antibody Separations

    • Robert L. Fahrner, Gregory S. Blank
    Pages 65-81

  6. Affinity-Based Interactions on Disks for Fast Analysis, Isolation and Conversion of Biomolecules

    • Laure G. Berruex, Ruth Freitag
    Pages 82-114

  7. Sugars as Affinity Ligands

    • Posettihalli R. Satish, Avadhesha Surolia
    Pages 115-129

  8. Macroaffinity Ligands in Bioseparation

    • Ipsita Roy, Munishwar N. Gupta
    Pages 130-147

  9. Cellulose-Binding Fusion Proteins

    • Alisdair B. Boraston, Bradley W. McLean, Mojgan Kavoosi, Charles A. Haynes, Douglas G. Kilburn
    Pages 148-162

  10. Two-Phase Affinity Partitioning of Animal Cells: Implications of Multipoint Interactions

    • Ashok Kumar, Masamichi Kamihira, Bo Mattiasson
    Pages 163-180

  11. Affinity-Based Reverse Micellar Separations

    • Motonari Adachi
    Pages 181-194

  12. Biosensors: An Emerging Tool in Bioanalytical Research — A Kinetic Analysis of the Analyte-Receptor Interactions in Biosensors

    • Anand Ramakrishnan, Ajit Sadana
    Pages 195-216

  13. Back Matter

    Pages 217-225
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Keywords

Editors and Affiliations

  • Department of Chemistry, Indian Institute of Technology, New Delhi, India

    Munishwar Nath Gupta

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