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Traumatic and Ischemic Injury

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Binu Tharakan

    1. Department of Surgery, Texas A&M University Health Science Center, College of Medicine, Baylor Scott and White Research Institute, Temple, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1717)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Mouse Injury Model of Polytrauma and Shock

    • Juan C. Mira, Dina C. Nacionales, Tyler J. Loftus, Ricardo Ungaro, Brittany Mathias, Alicia M. Mohr et al.
    Pages 1-15

  3. Measurement of Intracranial Pressure in Freely Moving Rats

    • Michael R. Williamson, Roseleen F. John, Frederick Colbourne
    Pages 17-25

  4. A Lateral Fluid Percussion Injury Model for Studying Traumatic Brain Injury in Rats

    • Paige S. Katz, Patricia E. Molina
    Pages 27-36

  5. A Mouse Controlled Cortical Impact Model of Traumatic Brain Injury for Studying Blood–Brain Barrier Dysfunctions

    • Himakarnika Alluri, Chinchusha Anasooya Shaji, Matthew L. Davis, Binu Tharakan
    Pages 37-52

  6. A Rat Model of Hemorrhagic Shock for Studying Vascular Hyperpermeability

    • Prince Esiobu, Ed W. Childs
    Pages 53-60

  7. Assessment of Cardiovascular Function and Microvascular Permeability in a Conscious Rat Model of Alcohol Intoxication Combined with Hemorrhagic Shock and Resuscitation

    • Travis M. Doggett, Jared J. Tur, Natascha G. Alves, Sarah Y. Yuan, Srinivas M. Tipparaju, Jerome W. Breslin
    Pages 61-81

  8. Intracerebral Hemorrhage in Mice

    • Damon Klebe, Loretta Iniaghe, Sherrefa Burchell, Cesar Reis, Onat Akyol, Jiping Tang et al.
    Pages 83-91

  9. A Rat Burn Injury Model for Studying Changes in Microvascular Permeability

    • Katie Wiggins-Dohlvik, Binu Tharakan
    Pages 93-100

  10. Modeling Transient Focal Ischemic Stroke in Rodents by Intraluminal Filament Method of Middle Cerebral Artery Occlusion

    • Mary Susan Lopez, Raghu Vemuganti
    Pages 101-113

  11. A Complete Guide to Using the Endothelin-1 Model of Stroke in Conscious Rats for Acute and Long-Term Recovery Studies

    • Hima C. S. Abeysinghe, Carli L. Roulston
    Pages 115-133

  12. A Murine Model of Hind Limb Ischemia to Study Angiogenesis and Arteriogenesis

    • Jun Yu, Alan Dardik
    Pages 135-143

  13. A Murine Model of Myocardial Ischemia–Reperfusion Injury

    • Zhaobin Xu, Kevin E. McElhanon, Eric X. Beck, Noah Weisleder
    Pages 145-153

  14. A Rat Model of Perinatal Seizures Provoked by Global Hypoxia

    • Jason A. Justice, Russell M. Sanchez
    Pages 155-159

  15. Experimental Protocol for Cecal Ligation and Puncture Model of Polymicrobial Sepsis and Assessment of Vascular Functions in Mice

    • Santosh Kumar Mishra, Soumen Choudhury
    Pages 161-187

  16. Methods to Study the Innate Immune Response to Sepsis

    • Wendy E. Walker
    Pages 189-206

  17. An Ovine Model for Studying the Pathophysiology of Septic Acute Kidney Injury

    • Yugeesh R. Lankadeva, Junko Kosaka, Roger G. Evans, Clive N. May
    Pages 207-218

  18. An In Vitro Model of Traumatic Brain Injury

    • Ellaine Salvador, Malgorzata Burek, Carola Y. Förster
    Pages 219-227

  19. An In Vitro Oxygen–Glucose Deprivation Model for Studying Ischemia–Reperfusion Injury of Neuronal Cells

    • Myoung-gwi Ryou, Robert T. Mallet
    Pages 229-235

  20. Measurement of Microvascular Endothelial Barrier Dysfunction and Hyperpermeability In Vitro

    • Bobby Darnell Robinson, Chinchusha Anasooya Shaji, Angela Lomas, Binu Tharakan
    Pages 237-242
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Keywords

About this book

This volume focuses on procedures for the development and application of several research animal models and in vitro methods that allow researchers to gain insight into the underlying cellular, biochemical, and physiological mechanisms involved in traumatic and ischemic injury. The chapters in this book discuss topics, such as animal models and techniques commonly used to study traumatic brain injury, hemorrhagic shock, ischemic and hemorrhagic strokes, sepsis, burn injury, hind limb ischemia, myocardial-ischemia-reperfusion injury, intracranial pressure, global hypoxia-induced perinatal seizures models, and in vitro models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Cutting-edge and comprehensive, Traumatic and Ischemic Injury: Methods and Protocols is a valuable resource for novices with limited experience to help them initiate new research projects, and established researchers to help them identify comparable approaches and strategies to their studies in this field.


Editors and Affiliations

  • Department of Surgery, Texas A&M University Health Science Center, College of Medicine, Baylor Scott and White Research Institute, Temple, USA

    Binu Tharakan

Bibliographic Information

  • Book Title: Traumatic and Ischemic Injury

  • Book Subtitle: Methods and Protocols

  • Editors: Binu Tharakan

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7526-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7524-2Published: 22 February 2018

  • Softcover ISBN: 978-1-4939-8510-4Published: 04 June 2019

  • eBook ISBN: 978-1-4939-7526-6Published: 21 February 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 245

  • Number of Illustrations: 50 b/w illustrations, 16 illustrations in colour

  • Topics: Molecular Medicine

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