Overview
- Features all the latest and hottest technology used in conventional and real-time RT-PCR
- Provides a broad scope covered in good depth, from detection of minute qualities of microRNA to the analysis of complex cell populations
- Serves as a comprehensive guide to setting up, running and analyzing an RT-PCR experiment
Part of the book series: Methods in Molecular Biology (MIMB, volume 630)
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About this book
Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.
User friendly and up-to-date, RT-PCR Protocols, Second Edition acts as a handy companion to scientists from numerous diverse backgrounds who wish to explore further the marvels of gene expression.
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Keywords
Table of contents (20 protocols)
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Front Matter
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The RT-PCR Detective: Hunting Down the Best Method
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Front Matter
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The RT-PCR Mathematician: Assessing Gene and RNA Expression
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The RT-PCR Master Chef: Finding the Right Ingredients
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Reviews
From the reviews of the second edition:
“The second edition of RT-PCR protocols and attempts to cover the most commonly used RT-PCR protocols, giving both beginners and experts a deeper understanding of the experimental set-up. … There are often flow diagrams or pictures to aid understanding and the references are helpful, if you require additional information. … a good investment for any lab interested in ensuring that their RT-PCR reactions are as productive and well-controlled as possible. … Overall a useful and detailed text written in an informative and helpful style.” (Hayley Evans, Immunology News, May, 2011)Editors and Affiliations
Bibliographic Information
Book Title: RT-PCR Protocols
Book Subtitle: Second Edition
Editors: Nicola King
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60761-629-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2010
Hardcover ISBN: 978-1-60761-628-3Published: 22 March 2010
Softcover ISBN: 978-1-4939-5682-1Published: 23 August 2016
eBook ISBN: 978-1-60761-629-0Published: 19 March 2010
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XI, 341
Topics: Human Genetics, Gene Expression