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This book reflects practical approaches to answering a variety of biological and medical questions using DNA fingerprinting and genetic profiling in a broad sense that also includes statistical evaluation of the data. It has been written for biology, biochemistry and medical students, graduates, postdoctor al fellows, academic teachers and for all researchers in the theoretical and ap plied sciences wherever genetic identification and relationship analyses are re quired. Although the book represents a holistic treatment of the "state-of-the art" technology concerning these topics, methological details are always given in the form of elaborated protocols so that the reader can benefit in terms of everyday work in the laboratoryas well. Jorg Thomas Epplen Thomas Lubjuhn Bochum, Germany Bonn, Germany January 1999 x Abbreviations 10 one-dimensional numtDNA nuclear-localized mitochondrial 2D two-dimensional DNA A260 absorption at 260 nm origin of H -strand replication A280 absorption at 280 nm origin of L-strand replication AFLP amplified fragment length poly polyacrylamide morphism polyacrylamide gel electrophor AP-PCR arbitrarily primed PCR esis BSA bovine serum albumin PAR pseUdo-autosomal region CE capillary electrophoresis PBL peripheral blood lymphocyte CNS central nervous system PCR polymerase chain reaction CaDIS combined DNA indexing system PFGE pulse field gel electrophoresis CpDNA chloroplastic DNA Phe phenylalanine CPU central processing unit PP polypropylene CTAB hexadecyltrimethyl ammonium PPVP polyvinyl polypyrrolidone bromide Pro proline DEAE diethylaminoethyl PVP polyvinylpyrrolidone DGGE denaturing gradient gel electro QTL quantitative trait locus phoresis RAPD random amplified polymorphic DHPLC denaturing high-pressure liquid DNA chromatography RFLP restriction fragment length poly DIG digoxigenin morphism
Content Level »Research
Keywords »Chromosom - DNA - PCR - Single Nucleotide Polymorphism - biology - chromosome - genetics - methodology
1. DNA Fingerprinting of Prokaryotic Genomes.- 2. Plant DNA Fingerprinting and Profiling.- 3. DNA Fingerprinting and Profiling in Behavioural Ecology.- 4. DNA Profiling in Veterinary Medicine.- 5. Multilocus DNA Fingerprinting.- 6. Various Levels of (Epi)Genetic Diversities as Demonstrable via Simple Repeated Sequences.- 7. Forensics: Analysis of Short Tandem Repeat Loci by Multiplex PCR and Real Time Fluorescence Detection During Capillary Electrophoresis.- 8. Mitochondrial DNA: Diversity Analysis and Possible Pitfalls.- 9. PCR-based DNA Profiling of Human Y Chromosomes.- 10. The Use of Imperfect Microsatellites for DNA Fingerprinting and Population Genetics.- 11. PCR-SSCP: A Method for Reliably Detecting Single Nucleotide Polymorphisms in Multifactorial Diseases.- 12. Amplified Fragment Length Polymorphisms: A Non-Random PCR-Based Technique for Multilocus Sampling.- 13. Two-Dimensional DNA Fingerprinting.- 14. Statistical Approaches and Methods in Population Genetics Using Microsatellite Data.- 15. Statistical Inference from DNA Evidence.- Guide to Solutions.- Guide to Protocols.- Troubleshooting Guide.