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Proteomics in Functional Genomics

Protein Structure Analysis

  • Book
  • © 2000

Overview

Editors:
  1. P. Jollès

    1. Laboratoire de Chimie des Substances Naturelles URA C.N.R.S. No. 401, Muséum National d’Histoire Naturelle, Paris, France

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  2. H. Jörnvall

    1. Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Experientia Supplementum (EXS, volume 88)

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Table of contents (14 chapters)

  1. Front Matter

    Pages I-XIII
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  2. Proteome mapping by two-dimensional polyacrylamide gel electrophoresis in combination with mass spectrometric protein sequence analysis

    • Ettore Appella, David Arnott, Kazuyasu Sakaguchi, Peter J. Wirth
    Pages 1-27

  3. Techniques for sample preparation including methods for concentrating peptide samples

    • Kris Gevaert, Tony Houthaeve, Joël Vandekerckhove
    Pages 29-42

  4. Sample preparation by SDS/PAGE and in-gel digestion

    • Ulf Hellman
    Pages 43-54

  5. High-resolution two-dimensional gel electrophoresis and protein identification using western blotting and ECL detection

    • Julio E. Celis, Pavel Gromov
    Pages 55-67

  6. Nanospray mass spectrometry in protein and peptide chemistry

    • William J. Griffiths
    Pages 69-79

  7. MALDI-TOF mass spectrometry in protein chemistry

    • Peter Roepstorff
    Pages 81-97

  8. The chemistry of protein sequence analysis

    • John E. Shively
    Pages 99-117

  9. The alkylated thiohydantoin method for C-terminal sequence analysis

    • David R. Dupont, MeriLisa Bozzini, Victoria L. Boyd
    Pages 119-131

  10. Ladder sequencing

    • Tomas Bergman
    Pages 133-144

  11. New approaches for innovations in sensitive Edman sequence analysis by design of a wafer-based chip sequencer

    • Christian Wurzel, Brigitte Wittmann-Liebold
    Pages 145-157

  12. Analysis of glycoprotein-derived glycopeptides

    • Gerrit J. Gerwig, Johannes F. G. Vliegenthart
    Pages 159-186

  13. Lipopeptide preparation and analysis

    • Jan Johansson, Margareta Stark, Magnus Gustafsson, Yuqin Wang, Shahparak Zaltash
    Pages 187-198

  14. Phosphopeptide analysis

    • Manfredo Quadroni, Peter James
    Pages 199-213

  15. Bioinformatics in protein analysis

    • Bengt Persson
    Pages 215-231

  16. Back Matter

    Pages 233-236
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Keywords

About this book

A wealth of information has accumulated over the last few years on the human genome. The new insights have completely changed the focus of protein analysis. It is no longer time-consuming analysis of unknown products, but rather selective identifications of individual forms, modifications and processings, and overall analysis of global protein outputs from cells and tissues in health and disease. This book gears to the rising need of sensitive, accurate, and fast separation and identification techniques in proteomics. It discusses current methodologies of modern protein analysis, from isolation and sample preparation, over analysis and identification, to final characterization. Several evaluations concentrate on the now productive approaches of two-dimensional gel electrophoresis and mass spectrometry, but alternative methods and further perspectives are also outlined. The book includes an overlook over current databases to connect protein analysis data with all available information,...

Editors and Affiliations

  • Laboratoire de Chimie des Substances Naturelles URA C.N.R.S. No. 401, Muséum National d’Histoire Naturelle, Paris, France

    P. Jollès

  • Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden

    H. Jörnvall

Bibliographic Information

  • Book Title: Proteomics in Functional Genomics

  • Book Subtitle: Protein Structure Analysis

  • Editors: P. Jollès, H. Jörnvall

  • Series Title: Experientia Supplementum

  • DOI: https://doi.org/10.1007/978-3-0348-8458-7

  • Publisher: Birkhäuser Basel

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Basel AG 2000

  • Hardcover ISBN: 978-3-7643-5885-3Published: 01 March 2000

  • Softcover ISBN: 978-3-0348-9576-7Published: 30 October 2012

  • eBook ISBN: 978-3-0348-8458-7Published: 11 March 2013

  • Series ISSN: 1664-431X

  • Series E-ISSN: 2504-3692

  • Edition Number: 1

  • Number of Pages: XIII, 236

  • Topics: Life Sciences, general, Biomedicine general

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