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Modern Analytical Ultracentrifugation

Acquisition and Interpretation of Data for Biological and Synthetic Polymer Systems

  • Book
  • © 1994

Overview

Editors:
  1. Todd M. Schuster

    1. Department of Molecular & Cell Biology and Analytical Ultracentrifugation Facility, University of Connecticut, Storrs, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Thomas M. Laue

    1. Department of Biochemistry, University of New Hampshire, Durham, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Emerging Biochemical and Biophysical Techniques (EBBT)

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Table of contents (17 chapters)

  1. Front Matter

    Pages i-xiv
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  2. Sedimentation Equilibrium

    1. Front Matter

      Pages 1-1
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    2. Notes on the Derivation of Sedimentation Equilibrium Equations

      • Hiroshi Fujita
      Pages 3-14

    3. Association of Rei Immunoglobulin Light Chain VL Domains: The Functional Linearity Of Parameters in Equilibrium Analytical Ultracentrifuge Models for Self-Associating Systems

      • Ian Brooks, Ronald Wetzel, Winnie Chan, Grace Lee, Donald G. Watts, K. Karl Soneson et al.
      Pages 15-36

    4. Comments on the Analysis of Sedimentation Equilibrium Experiments

      • Michael L. Johnson, Martin Straume
      Pages 37-65

    5. The Omega Analysis and the Characterization of Solute Self-Association by Sedimentation Equilibrium

      • Donald J. Winzor, Peter R. Wills
      Pages 66-80

    6. Conservation of Signal: A New Algorithm for the Elimination of the Reference Concentration as an Independently Variable Parameter in the Analysis of Sedimentation Equilibrium

      • Allen P. Minton
      Pages 81-93

    7. Analysis of Protein-Nucleic Acid and Protein-Protein Interactions Using Multi-Wavelength Scans from the XL-A Analytical Ultracentrifuge

      • Marc S. Lewis, Richard I. Shrager, Soon-Jong Kim
      Pages 94-115

  3. Sedimentation Velocity

    1. Front Matter

      Pages 117-117
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    2. Sedimentation Boundary Analysis of Interacting Systems: Use of the Apparent Sedimentation Coefficient Distribution Function

      • Walter F. Stafford III
      Pages 119-137

    3. Studies of Macromolecular Interaction by Sedimentation Velocity

      • James C. Lee, Surendran Rajendran
      Pages 138-155

    4. Measuring Sedimentation, Diffusion, and Molecular Weights of Small Molecules by Direct Fitting of Sedimentation Velocity Concentration Profiles

      • John S. Philo
      Pages 156-170

    5. Computer Simulation of the Sedimentation of Ligand-Mediated and Kinetically Controlled Macromolecular Interactions

      • John R. Cann
      Pages 171-188

    6. Refining Hydrodynamic Shapes of Proteins: The Combination of Data From Analytical Ultracentrifugation and Time-Resolved Fluorescence Anisotropy Decay

      • Evan Waxman, William R. Laws, Thomas M. Laue, J. B. Alexander Ross
      Pages 189-205

  4. Acquisition and Data Reduction

    1. Front Matter

      Pages 207-207
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    2. On Line Data Acquisition for the Rayleigh Interference Optical System of the Analytical Ultracentrifuge

      • David A. Yphantis, Jeffrey W. Lary, Walter F. Stafford, Sen Liu, Philip H. Olsen, David B. Hayes et al.
      Pages 209-226

    3. Extensions to Commercial Graphics Packages for Customization of Analysis of Analytical Ultracentrifuge Data

      • Joe Hedges, Shokoh Sarrafzadeh, James D. Lear, Donald K. McRorie
      Pages 227-244

    4. A Graphical Method for Determining the Ideality of a Sedimenting Boundary

      • David B. Hayes, Thomas M. Laue
      Pages 245-258

  5. Some Specific Examples

    1. Front Matter

      Pages 259-259
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    2. Analytical Ultracentrifugation and its use in Biotechnology

      • Steven J. Shire
      Pages 261-297
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Keywords

About this book

There are numerous examples in the history of science when the parallel develop­ ments of two or more disciplines, methodologies, technologies or theoretical in­ sights have converged to produce significant scientific advances. The decades following the 1950s have produced several such significant advances, as a result of a convergence of developments in molecular biology and in solid state-based electronics instrumentation. Since one of these areas of significant advancement, analytical ultracentrifu­ gation, has been undergoing a renaissance, we thought it would be a useful activity to call upon a group of researchers who have been developing either the experi­ mental or theoretical aspects of the methodology and gather in one place a group of articles summarizing the current status of the field. The success of recombinant DNA methodologies at producing biologically active macromolecules of commer­ cial interest has evoked interests in mechanisms of function. Pursuit of the related questions has emphasized the importance of studies of macromolecular binding and interaction. Several contributions to this volume remind us that analytical ultra­ centrifugation is rigorously based on solid thermodynamic theory and, as such, is fully capable of providing comprehensive quantitative descriptions of molecular interactions in solution. Furthermore, a number of the chapters provide examples, along with innovative methods for carrying out these characterizations. The past decade has seen several developments that reflect the rebirth of interest in analytical ultracentrifugation.

Editors and Affiliations

  • Department of Molecular & Cell Biology and Analytical Ultracentrifugation Facility, University of Connecticut, Storrs, USA

    Todd M. Schuster

  • Department of Biochemistry, University of New Hampshire, Durham, USA

    Thomas M. Laue

Bibliographic Information

  • Book Title: Modern Analytical Ultracentrifugation

  • Book Subtitle: Acquisition and Interpretation of Data for Biological and Synthetic Polymer Systems

  • Editors: Todd M. Schuster, Thomas M. Laue

  • Series Title: Emerging Biochemical and Biophysical Techniques

  • DOI: https://doi.org/10.1007/978-1-4684-6828-1

  • Publisher: Birkhäuser Boston, MA

  • eBook Packages: Springer Book Archive

  • Copyright Information: Birkhäuser Boston 1994

  • Softcover ISBN: 978-1-4684-6830-4Published: 01 March 2012

  • eBook ISBN: 978-1-4684-6828-1Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XIV, 354

  • Topics: Life Sciences, general, Biomedicine general

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