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Biomedical Sciences - Pharmacology & Toxicology | Pharmacogenomics - Methods and Protocols

Pharmacogenomics

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1015

Innocenti, Federico, Schaik, Ron H.N. (Eds.)

2nd ed. 2013, XIII, 359 p. 56 illus., 33 illus. in color.

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  • Fully updated from the popular first edition
  • Features easy-to-follow protocols that are ready for the laboratory 
  • Contains expert tips and key implementation advice to ensure successful results 

Understanding an individual's genetic makeup is the key to creating personalized drugs with greater efficacy and safety, and pharmacogenomics aims to study the complex genetic basis of inter-patient variability in response to drug therapy.  Based upon the success of its first edition, the second edition of Pharmacogenomics: Methods And Protocols aims to continue providing readers with high-quality content on the most innovative and commonly adopted technologies in the field of pharmacogenomics as presented by experts in the field.  Broken into several sections, this detailed volume examines techniques for interrogating variation in human genes and genomes, functional assessment of genetic variation, both in vitro and in vivo, as well as tools for translation and implementation of pharmacogenetic markers. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to the respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and thoroughly updated, Pharmacogenomics: Methods And Protocols, Second Edition serves as an essential reference and an invaluable source on the latest information in this field.

Content Level » Professional/practitioner

Keywords » Drug therapy - Individual genetic makeup - Personalized medicine - Pharmaceutical design - Pharmacogenomic technologies

Related subjects » Human Genetics - Pharmacology & Toxicology

Table of contents 

Part I: Introduction to Pharmacogenomics

 

1. Pharmacogenomics: Historical Perspective and Current Status

            Rosane Charlab and Lei  Zhang

 

Part II: Techniques for Interrogating Variation in Human Genes and Genomes

 

2. Denaturing High-Performance Liquid Chromatography for Mutation Detection and Genotyping

            Donna Lee Fackenthal, Pei Xian Chen, Ted Howe, and Soma Das

 

3. Clinical SNP Detection by the SmartAmp Method

            Toshihisa Ishikawa and Yoshihide Hayashizaki

 

4. MALDI-TOF Mass Spectrometry

            Dirk van den Boom, Matthias Wjst, and Robin Everts

 

5. TaqMan® Drug Metabolism Genotyping Assays for the Detection of Human Polymorphisms Involved in Drug Metabolism

            Toinette Hartshorne

 

6. Pyrosequencing of Clinically Relevant Polymorphisms

            Cristi R. King and Sharon Marsh

 

7. Pharmacogenetics Using Luminex® xMAP® Technology: Method for Developing a Custom Multiplex Single Nucleotide Polymorphism Mutation Assay

            Gonnie Spierings and Sherry A. Dunbar

 

8. Use of Linkage Analysis, Genome-Wide Association Studies, and Next-Generation Sequencing in the Identification of Disease-Causing Mutations

            Eric Londin, Priyanka Yadav, Saul Surrey, Larry J. Kricka, and Paolo Fortina

 

9. The GoldenGate Genotyping Assay: Custom Design, Processing, and Data Analysis

            Anna González-Neira

 

10. Genome-Wide Gene Expression Profiling, Genotyping, and Copy Number Analyses of Acute Myeloid Leukemia Using Affymetrix GeneChips

            Mathijs A. Sanders and Peter J.M. Valk

 

11. Epigenetic Techniques in Pharmacogenetics

            Sandra G. Heil

 

12. Plasmid Derived External Quality Controls for Genetic Testing

            Tahar van der Straaten and Henk-Jan Guchelaar

 

Part III: Functional Assessment of Genetic Variation: In Vitro and In Vivo Methods

 

13. Allelic Imbalance Assays to Quantify Allele-Specific Gene Expression and Transcription Factor Binding

            Francesca Luca and Anna Di Rienzo

 

14. SCAN: A Systems Biology Approach to Pharmacogenomic Discovery

            Eric R. Gamazon, R. Stephanie Huang, and Nancy J. Cox

 

15. Methods to Examine the Impact of Nonsynonymous SNPs on Protein Degradation and Function of Human ABC Transporter

            Toshihisa Ishikawa, Kanako Wakabayashi-Nakao, and Hiroshi Nakagawa

 

16. In Vitro Identification of Cytochrome P450 Enzymes Responsible for Drug Metabolism

            Zhengyin Yan and Gary W. Caldwell

 

17. In Vitro and In Vivo Mouse Models for Pharmacogenetic Studies

            Amber Frick, Oscar Suzuki, Natasha Butz, Emmanuel Chan, and Tim Wiltshire

 

18. The Hydrodynamic Tail Vein Assay as a Tool for the Study of Liver Promoters and Enhancers

            Mee J. Kim and Nadav Ahituv

 

Part IV: Tools for Translation and Implementation of Pharmacogenetic Markers

 

19. A Guide to the Current Web-Based Resources in Pharmacogenomics

            Dylan M. Glubb, Steven W. Paugh, Ron H.N. van Schaik, and Federico Innocenti

 

20. PharmGKB: The Pharmacogenomics Knowledge Base

            Caroline F. Thorn, Teri E. Klein, and Russ B. Altman

 

21. Genetic Databases in Pharmacogenomics: The Frequency of Inherited Disorders Database (FINDbase)

            Marianthi Georgitsi and George P. Patrinos

 

22. Development of Predictive Models for Estimating Warfarin Maintenance Dose Based on Genetic and Clinical Factors

            Lu Yang and Mark W. Linder

 

23. Evidence Based Drug Dosing and Pharmacotherapeutic Recommendations Per Genotype

            Vera H.M. Deneer and Ron H.N. van Schaik on behalf of the Dutch Pharmacogenetics Working Group

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