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Biomedical Sciences - Pharmacology & Toxicology | Glycosylation Engineering of Biopharmaceuticals - Methods and Protocols

Glycosylation Engineering of Biopharmaceuticals

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 988

Beck, Alan (Ed.)

2013, XV, 355 p. 111 illus., 45 illus. in color.

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  • Presents detailed protocols on the production and characterization of glycoproteins and glyco-engineered biopharmaceuticals
  • Contains a special focus on monoclonal antibodies (mAbs)
  • Features expert tips and key implementation advice to guarantee successful results

Glyco-engineering is being developed as a method to control the composition of carbohydrates and to enhance the pharmacological properties of monoclonal antibodies (mAbs) and other proteins.  In Glycosylation Engineering of Biopharmaceuticals: Methods and Protocols, experts in the field provide readers with production and characterization protocols of glycoproteins and glyco-engineered biopharmaceuticals with a focus on mAbs. The volume is divided in four complementary parts dealing with glyco-engineering of therapeutic proteins, glycoanalytics, glycoprotein complexes characterization, and PK/PD assays for therapeutic antibodies.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Glycosylation Engineering of Biopharmaceuticals: Methods and Protocols serves as an ideal guide for scientists striving to push forward the exciting field of engineered biopharmaceuticals.

Content Level » Professional/practitioner

Keywords » Biopharmaceuticals - Glyco-engineering - Glycoanalytics - Glycoproteins - Monoclonal antibodies - PK/PD assays - Therapeutic proteins - mAbs

Related subjects » Biotechnology - Pharmacology & Toxicology

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