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Biomedical Sciences - Pharmacology & Toxicology | In Vitro Neurotoxicology - Methods and Protocols

In Vitro Neurotoxicology

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 758

Costa, Lucio G., Giordano, Gennaro, Guizzetti, Marina (Eds.)

2011, XII, 415p. 66 illus..

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  • Serves a wide audience seeking to assess or characterize potential neurotoxicity
  • Includes detailed, lab-ready protocols for numerous model system, both mammalian and non-mammalian
  • Features key tips and expert implementation advice for ideal results

In recent years, the need to develop acceptable alternatives to conventional animal testing for neurotoxicity and developmental neurotoxicity has been increasingly recognized, and much effort is being directed toward the development of alternative models, utilizing mostly mammalian cells in culture but also non-mammalian model systems.  In Vitro Neurotoxicology: Methods and Protocols presents a series of cellular, biochemical, and molecular methodological protocols in the area of in vitro neurotoxicology, with an emphasis on mammalian cell culture systems.  Opening with a section on methodologies for preparing several cellular systems of variable complexity, amenable for in vitro neurotoxicological studies, the thorough volume continues with coverage of methods to measure cellular death and major mechanisms, methods for assessing mechanisms of nervous system cell toxicity related to impairment of cell signaling, while a final section illustrates additional methods for assessing important nervous system processes such as cell proliferation, neuritogenesis, and synaptogenesis.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and cutting-edge, In Vitro Neurotoxicology: Methods and Protocols serves researchers with an interest in assessing or characterizing the potential neurotoxicity of environmental contaminants, drugs, or other chemicals.

Content Level » Professional/practitioner

Keywords » Cell signaling impairment - Cellular death - Chemicals - Drugs - Environmental contaminants - Mammalian cell culture - Model systems - Nervous system cell toxicity - Neurotoxicity - Pharmaceuticals

Related subjects » Neuroscience - Pharmacology & Toxicology

Table of contents 

1. In Vitro Neurotoxicology: An Introduction

            Lucio G. Costa, Gennaro Giordano, and Marina Guizzetti

 

Part I: Model Systems

 

2. Primary Neurons in Culture and Neuronal Cell Lines for In Vitro Neurotoxicological Studies

            Gennaro Giordano and Lucio G. Costa

 

3. Determination of Metal Interactions with the Chaperone Hspa5 in Human Astrocytoma Cells and Rat Astrocyte Primary Cultures

            Evelyn Tiffany-Castiglioni, Yongchang Qian, Rola Barhoumi, and Ying Zheng

 

4. Microglia Cell Culture: A Primer for the Novice

            Anke Witting and Thomas Möller

 

5. Neural Stem Cells for Developmental Neurotoxicity Studies

            Roshan Tofighi, Michaela Moors, Raj Bose, Wan Norhamidah Wan Ibrahim, and Sandra Ceccatelli

 

6. Preparation, Maintenance, and Use of Serum-Free Aggregating Brain Cell Cultures

            Paul Honegger, Antoinette Defaux, Florianne Monnet-Tschudi, and Marie-Gabrielle Zurich

 

7. Neurospheres as a Model for Developmental Neurotoxicity Testing

            Ellen Fritsche, Kathrin Gassmann, and Timm Schreiber

 

8. Acute Hippocampal Slice Preparation and Hippocampal Slice Cultures

            Pamela J. Lein, Christopher D. Barnhart, and Isaac N. Pessah

 

9. Glia-Neuron Sandwich Co-Cultures: An In Vitro Approach to Evaluate Cell-to-Cell Communication in Neuroinflammation and Neurotoxicity

            Mariaserena Boraso and Barbara Viviani

 

10. In Vitro Models to Study the Blood Brain Barrier

            Hilary Vernon, Katherine Clark, and Joseph P. Bressler

 

Part II: Cell Death

 

11. Measurements of Cell Death in Neuronal and Glial Cells

            Gennaro Giordano, Sungwoo Hong , Elaine M. Faustman, and Lucio G. Costa

 

12. Measurements of Neuronal Apoptosis

            Gennaro Giordano and Lucio G. Costa

 

13. Measurement of Isoprostanes as Markers of Oxidative Stress

            Dejan Milatovic, Thomas J. Montine, and Michael Aschner

 

14. Assessment of Glutathione Homeostasis

            Gennaro Giordano, Collin C.  White, and Lucio G. Costa

 

15. Assessing Neuronal Bioenergetic Status

            Stephanie L.H. Zeiger, Jeannette N. Stankowski, and BethAnn McLaughlin

 

Part III: Signaling in Neurotoxicity

 

16. Fluorescent Assessment of Intracellular Calcium in Individual Cells

            David P. Cox, Collin C. White, and Terrance J. Kavanagh

 

17. Homeostatic Regulation of Glutamate Neurotransmission in Primary Neuronal Cultures

            Victor Briz and Cristina Suñol

 

18. Detection of Nitric Oxide Formation in Primary Neural Cells and Tissues

            Ronald B. Tjalkens, David L. Carbone, and Guoyao Wu

 

19. JNK3-Mediated Apoptotic Cell Death in Primary Dopaminergic Neurons

            Won-Seok Choi, Heather M Klintworth, and Zhengui Xia

 

20. Measurement of Proteasomal Dysfunction in Cell Models of Dopaminergic Degeneration

            Faneng Sun, Anumantha G. Kanthasamy, and Arthi Kanthasamy

 

21. Cell Signaling and Neurotoxicity: Protein Kinase C In Vitro and In Vivo

            Prasada Rao S. Kodavanti

 

22. Cell Signaling and Neurotoxicity: 3H-Arachidonic Acid Release (Phospholipase A2) in Cerebellar Granule Neurons

            Prasada Rao S. Kodavanti

 

Part IV: Other End-Points

 

23. Quantitative Assessment of Neurite Outgrowth in PC12 Cells

            Joshua A. Harrill and William R. Mundy

 

24. Measurements of Astrocyte Proliferation

            Marina Guizzetti, Terrance J. Kavanagh, and Lucio G. Costa

 

25. Quantification of Synaptic Structure Formation in Co-Cultures of Astrocytes and Hippocampal Neurons

            Pamela J. Roqué, Marina Guizzetti, Gennaro Giordano, and Lucio G. Costa

 

26. Volume Measurements in Cultured Primary Astrocytes

            Michael Aschner

 

27. Assessment of Cholesterol Homeostasis in Astrocytes and Neurons

            Jing Chen, Lucio G. Costa, and Marina Guizzetti

 

28. Dose-Response or Dose-Effect Curves in In Vitro Experiments and Their Use to Study Combined Effects of Neurotoxicants

            Matteo Goldoni and Sara Tagliaferri

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