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Structural Genomics and Drug Discovery

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Wayne F. Anderson

    1. Center for Structural Genomics of Infectious Diseases Midwest Center for Structural Genomics, Northwestern University Feinberg School of Medicine, Chicago, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1140)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Data Management in the Modern Structural Biology and Biomedical Research Environment

    • Matthew D. Zimmerman, Marek Grabowski, Marcin J. Domagalski, Elizabeth M. MacLean, Maksymilian Chruszcz, Wladek Minor
    Pages 1-25

  3. Structural Genomics of Human Proteins

    • Khan Tanjid Osman, Aled Edwards
    Pages 27-34

  4. Target Selection for Structural Genomics of Infectious Diseases

    • Corin Yeats, Benoit H. Dessailly, Elizabeth M. Glass, Daved H. Fremont, Christine A. Orengo
    Pages 35-51

  5. Selecting Targets from Eukaryotic Parasites for Structural Genomics and Drug Discovery

    • Isabelle Q. H. Phan, Robin Stacy, Peter J. Myler
    Pages 53-59

  6. High-Throughput Cloning for Biophysical Applications

    • Keehwan Kwon, Scott N. Peterson
    Pages 61-74

  7. Expression and Solubility Testing in a High-Throughput Environment

    • Keehwan Kwon, Scott N. Peterson
    Pages 75-88

  8. Protein Production for Structural Genomics Using E. coli Expression

    • Magdalena Makowska-Grzyska, Youngchang Kim, Natalia Maltseva, Hui Li, Min Zhou, Grazyna Joachimiak et al.
    Pages 89-105

  9. Eukaryotic Expression Systems for Structural Studies

    • Christopher A. Nelson, William H. McCoy 4th, Daved H. Fremont
    Pages 107-116

  10. Automated Cell-Free Protein Production Methods for Structural Studies

    • Emily T. Beebe, Shin-ichi Makino, John L. Markley, Brian G. Fox
    Pages 117-135

  11. Parallel Protein Purification

    • Ludmilla Shuvalova
    Pages 137-143

  12. Oxidative Refolding from Inclusion Bodies

    • Christopher A. Nelson, Chung A. Lee, Daved H. Fremont
    Pages 145-157

  13. High-Throughput Crystallization Screening

    • Tatiana Skarina, Xiaohui Xu, Elena Evdokimova, Alexei Savchenko
    Pages 159-168

  14. Screening Proteins for NMR Suitability

    • Adelinda A. Yee, Anthony Semesi, Maite Garcia, Cheryl H. Arrowsmith
    Pages 169-178

  15. Salvage or Recovery of Failed Targets by In Situ Proteolysis

    • Yufeng Tong, Aiping Dong, Xiaohui Xu, Amy Wernimont
    Pages 179-188

  16. Salvage of Failed Protein Targets by Reductive Alkylation

    • Kemin Tan, Youngchang Kim, Catherine Hatzos-Skintges, Changsoo Chang, Marianne Cuff, Gekleng Chhor et al.
    Pages 189-200

  17. Salvage or Recovery of Failed Targets by Mutagenesis to Reduce Surface Entropy

    • Lukasz Goldschmidt, David Eisenberg, Zygmunt S. Derewenda
    Pages 201-209

  18. Data Collection for Crystallographic Structure Determination

    • Kanagalaghatta Rajashankar, Zbigniew Dauter
    Pages 211-237

  19. Structure Determination, Refinement, and Validation

    • George Minasov, Wayne F. Anderson
    Pages 239-249

  20. Virtual High-Throughput Ligand Screening

    • T. Andrew Binkowski, Wei Jiang, Benoit Roux, Wayne F. Anderson, Andrzej Joachimiak
    Pages 251-261
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Keywords

About this book

Structural Genomics and Drug Discovery: Methods and Protocols focuses on high throughput structure determination methods and how they can be applied to lay the groundwork for structure aided drug discovery. The methods and protocols that are described can be applied in any laboratory interested in using detailed structural information to advance the initial stages of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Structural Genomics and Drug Discovery: Methods and Protocols seeks to aid scientists in the further study into structural genomics approach as an efficient initial step toward drug discovery and the methods described will be useful to anyone interested in moving in this direction.

Editors and Affiliations

  • Center for Structural Genomics of Infectious Diseases Midwest Center for Structural Genomics, Northwestern University Feinberg School of Medicine, Chicago, USA

    Wayne F. Anderson

Bibliographic Information

  • Book Title: Structural Genomics and Drug Discovery

  • Book Subtitle: Methods and Protocols

  • Editors: Wayne F. Anderson

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0354-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2014

  • Hardcover ISBN: 978-1-4939-0353-5Published: 04 March 2014

  • Softcover ISBN: 978-1-4939-4462-0Published: 30 April 2017

  • eBook ISBN: 978-1-4939-0354-2Published: 08 July 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 344

  • Number of Illustrations: 14 b/w illustrations, 64 illustrations in colour

  • Topics: Pharmacology/Toxicology, Human Genetics

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