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Comprehensively summarizes the different approaches to assess drug absorption from in vitro models
Provides readers with a review of the current state-of-the-art in pharmaceutical cell biology
Provides readers with helpful protocols for many applications that can be directly used at the laboratory bench
This is a well thought-out, highly practical text covering contemporary ‘in vitro’ techniques for drug absorption studies. It will be essential reading for any number of professionals in drug research.
Starting at the molecular level of investigation, it continues with cell monolayer models (both primary and cell lines) and culminates with in situ techniques as a final testing format.
In addition, chapters on high-throughput assays, in vitro-in vivo correlation, bioinformatics and regulatory issues are covered, giving a comprehensive overview of available models and techniques.
What’s more, an appendix consisting of a number of practical protocols is available online, updated as needed, and should prove very helpful to apply the techniques directly to the benchside.
Compared to the complexities of in vivo studies, in vitro models offer a fast, convenient approach with cost advantages most of times. Most importantly, they can be standardized and automatized to be applicable to the high-throughput screening.
Perfused Organ Level/In Situ Techniques.- Models for Skin Absorption and Skin Toxicity Testing.- Models of the Small Intestine.- Drug Absorption from the Colon In Situ.- In Vivo and In Vitro Models for Assessing Drug Absorption Across the Buccal Mucosa.- In Situ and Ex Vivo Nasal Models for Preclinical Drug Development Studies.- The Isolated Perfused Lung for Drug Absorption Studies.- Cellular Level—In Vitro Models of Epithelial and Endothelial Barriers.- In Vitro Models for Investigations of Buccal Drug Permeation and Metabolism.- In Vitro Screening Models to Assess Intestinal Drug Absorption and Metabolism.- In Vitro Cellular Models for Nasal Drug Absorption Studies.- In Vitro Models of the Tracheo-Bronchial Epithelium.- In Vitro Models of the Alveolar Epithelial Barrier.- Cell Culture Models of the Corneal Epithelium and Reconstructed Cornea Equivalents for In Vitro Drug Absorption Studies.- The Conjunctival Barrier in Ocular Drug Delivery.- Inner Blood—Retinal Barrier: Transport Biology and Methodology.- Regulation of Paracellular Permeability in Low-Resistance Human Vaginal-Cervical Epithelia.- In Vitro Models and Multidrug Resistance Mechanisms of the Placental Barrier.- In Vitro Models to Study Blood-Brain Barrier Function.- High-Throughput Screening Using Caco-2 Cell and PAMPA Systems.- Instrumented In Vitro Approaches to Assess Epithelial Permeability of Drugs from Pharmaceutical Formulations.- Bioinformatics—In Silico Tools to Predict Drug Absorption.- Modeling Transdermal Absorption.- Physiologically Based in Silico Models for the Prediction of Oral Drug Absorption.- In Silico Modeling for Blood—Brain Barrier Permeability Predictions.- Molecular/Sub-Cellular Level—Mechanistic Tools.- Impact of Drug Transport Proteins.- Cloning and Functional Heterologous Expression of Transporters.- The Pharmacology of Caveolae.- Immortalization Strategies for Epithelial Cells in Primary Culture.- Binding-Uptake Studies and Cellular Targeting.- Regulatory Considerations.- Drug Permeability Studies in Regulatory Biowaiver Applications.