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Biomedical Sciences - Neuroscience | Brain Development - Methods and Protocols

Brain Development

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1082

Sprecher, Simon G. (Ed.)

2014, X, 317 p. 63 illus., 49 illus. in color.

A product of Humana Press
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  • Introduces cutting-edge techniques in developmental neurobiology through genetic model systems as well as some complimentary animal models
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and expert implementation advice

Providing widely used techniques in genetic model systems and many complementing animal models, Brain Development: Methods and Protocols focuses its expert contributions on two key technical aspects of developmental neurobiology: detection of gene expression and functional characterization of developmental control genes.  Covering animal models such as the fruit fly, zebra fish, chicken, and mouse, this detailed book views in situ hybridization, reporter gene expression, and immunohistochemical staining methods, as well as RNA interference, Morpholino, or transgenic techniques through the prism of these models.  Written in the highly successful Methods in Molecular Biology series format, chapter include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Practical and cutting-edge, Brain Development: Methods and Protocols aims to provide precise technical protocols but also allows for comparing a wide range of protocols in different tissues and species.

Content Level » Professional/practitioner

Keywords » Animal models - Developmental control genes - Developmental neurobiology - Drosophila - Gene expression detection - Genetic model systems - Zebrafish

Related subjects » Neuroscience

Table of contents 

Part I: Drosophila Protocols


1. Immunostaining of the Developing Embryonic and Larval Drosophila Brain

            Danielle C. Diaper and Frank Hirth


2. Non-Fluorescent RNA In Situ-Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila

            David Jussen and Rolf Urbach


3. Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI-Labeling

            Karoline F. Kraft and Rolf Urbach


4. Flybow to Dissect Circuit Assembly in the Drosophila Brain

            Nana Shimosako, Dafni Hadjieconomou, and Iris Salecker


5. Immunofluorescent Labeling of Neural Stem Cells in the Drosophila Optic Lobe

            Benjamin Perruchoud and Boris Egger


6. Using MARCM to Study Drosophila Brain Development

            Gudrun Viktorin


Part II: Other Arthropods


7. Dye Coupling and Immunostaining of Astrocyte-Like Glia Following Intracellular Injection of Fluorochromes in Brain Slices of the Grasshopper, Schistocerca gregaria

            George Boyan and Yu Liu


Part III: Molluscs


8. Methods in Brain Development of Molluscs

            Andreas Wanninger and Tim Wollesen


Part IV: Xenopus Protocols


9. In Situ Hybridization and Immunostaining of Xenopus Brain

            Kai-li Liu, Xiu-mei Wang, Zi-long Li, and Ying Liu


10. Microinjection Manipulations in the Elucidation of Xenopus Brain Development

            Cristine Smoczer, Lara Hooker, Saqib S. Sachani, and Michael J. Crawford


11. Morpholino Studies in Xenopus Brain Development

            Jennifer E. Bestman and Hollis T. Cline


Part V: Zebrafish Protocols


12. Sensitive Whole-Mount Fluorescent In Situ Hybridization in Zebrafish Using Enhanced Tyramide Signal Amplification

            Gilbert Lauter, Iris Söll, and Giselbert Hauptmann


13. Dynamic Neuroanatomy at Subcellular Resolution in the Zebrafish

            Adèle Faucherre and Hernán López-Schier


14. Anatomical Dissection of Zebrafish Brain Development

            Katherine J. Turner, Thomas G. Bracewell, and Thomas A. Hawkins


Part VI: Chicken Protocols


15. Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain

            Richard P. Tucker and Qizhi Gong


16. Transplantation of Neural Tissue: Quail-Chick Chimeras

            Andrea Streit and Claudio D. Stern


17. RNAi-Based Gene Silencing in Chicken Brain Development

            Irwin Andermatt and Esther T. Stoeckli


Part VII: Mouse Protocols


18. Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development

            Jinling Liu and Aimin Liu


19. In Utero Electroporation to Study Mouse Brain Development

            Emilie Pacary and François Guillemot


20. The Cre/Lox System to Assess the Development of the Mouse Brain

            Claudius F. Kratochwil and Filippo M. Rijli

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