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Neural Development

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Renping Zhou

    1. , Department of Chemical Biology, Rutgers University, Piscataway, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Lin Mei

    1. , Inst. Molecular Medicine & Genetics, Georgia Health Sciences University, Augusta, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes clear and current techniques for the study of neural development
  • Provides step-by-step detail essential for reproducible results
  • Contains key tips and expert implementation advice to ensure success in the lab

Part of the book series: Methods in Molecular Biology (MIMB, volume 1018)

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Table of contents (31 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Neuron and Glial Cell Culture

    1. Front Matter

      Pages 1-1
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    2. Isolation and Maintenance of Cortical Neural Progenitor Cells In Vitro

      • Mi-Yeon Kim, Byoung-San Moon, Kang-Yell Choi
      Pages 3-10

    3. Neural Induction with a Dopaminergic Phenotype from Human Pluripotent Stem Cells Through a Feeder-Free Floating Aggregation Culture

      • Asuka Morizane, Daisuke Doi, Jun Takahashi
      Pages 11-19

    4. Nucleoside Analog Labeling of Neural Stem Cells and Their Progeny

      • Erik Michael DeBoer, Mladen-Roko Rasin
      Pages 21-37

    5. Culture of Dissociated Hippocampal Neurons

      • Yun Peng, Wen-Cheng Xiong, Lin Mei
      Pages 39-47

    6. Culturing Mouse Cerebellar Granule Neurons

      • Tharakeswari Selvakumar, Daniel L. Kilpatrick
      Pages 49-59

    7. Preparation of Primary Cultured Dopaminergic Neurons from Mouse Brain

      • Won-Seok Choi, Hyung-Wook Kim, Zhengui Xia
      Pages 61-69

    8. Culturing Astrocytes from Postnatal Rats

      • Yangyang Huang, Cheryl F. Dreyfus
      Pages 71-80

    9. Culturing Oligodendrocyte Lineage Cells from Neonatal Rats

      • Clifton G. Fulmer, Cheryl F. Dreyfus
      Pages 81-91

    10. Isolation and Culture of Schwann Cells

      • Yanmei Tao
      Pages 93-104

  3. Gene Expression and Analysis

    1. Front Matter

      Pages 105-105
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    2. DNA Transfection: Calcium Phosphate Method

      • Munjin Kwon, Bonnie L. Firestein
      Pages 107-110

    3. The Gene-Gun Approach for Transfection and Labeling of Cells in Brain Slices

      • Anna Dunaevsky
      Pages 111-118

    4. Lentiviral Vector Production, Titration, and Transduction of Primary Neurons

      • Baojin Ding, Daniel L. Kilpatrick
      Pages 119-131

    5. In Ovo Electroporation in Embryonic Chick Spinal Cords

      • Hui Wang, Michael P. Matise
      Pages 133-140

    6. Gene Transfer in Developing Chick Embryos: In Ovo Electroporation

      • Emma K. Farley
      Pages 141-150

    7. In Utero Electroporation in Mice

      • Chunlei Wang, Lin Mei
      Pages 151-163

    8. General Introduction to In Situ Hybridization Protocol Using Nonradioactively Labeled Probes to Detect mRNAs on Tissue Sections

      • Daehoon Lee, Shan Xiong, Wen-Cheng Xiong
      Pages 165-174

    9. Immunofluorescence Staining with Frozen Mouse or Chick Embryonic Tissue Sections

      • Hui Wang, Michael P. Matise
      Pages 175-188
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Keywords

About this book

Understanding the molecular and cellular mechanisms underlying the development of specific neural circuits is not just an intellectual curiosity but also central to our ability to develop therapeutic approaches to repair damaged pathways in the future. In Neural Development: Methods and Protocols, experts in the field contribute commonly used protocols to facilitate future research in developmental neuroscience. Split into four convenient sections, this detailed volume covers techniques of culturing neurons and glia as well as their growth and differentiation, methods of gene delivery and down regulation, protocols for analyzing axon growth and guidance plus synapse formation, and, finally, basic methods to analyze brain morphology and axon pathways in developing animals.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and accessible, Neural Development: Methods and Protocols provides key guidance for students and postdoctoral fellows new to developmental neurobiology.

Editors and Affiliations

  • , Department of Chemical Biology, Rutgers University, Piscataway, USA

    Renping Zhou

  • , Inst. Molecular Medicine & Genetics, Georgia Health Sciences University, Augusta, USA

    Lin Mei

Bibliographic Information

  • Book Title: Neural Development

  • Book Subtitle: Methods and Protocols

  • Editors: Renping Zhou, Lin Mei

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-444-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-443-2Published: 17 May 2013

  • Softcover ISBN: 978-1-4939-6018-7Published: 23 August 2016

  • eBook ISBN: 978-1-62703-444-9Published: 17 May 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 347

  • Number of Illustrations: 11 b/w illustrations, 48 illustrations in colour

  • Topics: Neurosciences, Neurobiology, Cell Culture

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