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Biomedical Sciences - Neuroscience | Dopamine - Methods and Protocols


Methods and Protocols

Series: Methods in Molecular Biology, Vol. 964

Kabbani, Nadine (Ed.)

2013, XI, 312 p. 78 illus., 22 illus. in color.

A product of Humana Press
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  • Focuses on leading, current approaches in the study of dopamine within a variety of biological systems
  • Features detailed protocols that have been tested in laboratories around the world
  • Includes expert tips and key implementation advice to ensure successful results

Dopamine, a catecholamine transmitter, plays a number of vital physiological roles in the brain and body, and, in recent years, studies on the role of dopamine in disease have opened new avenues of research and discovery.  In Dopamine: Methods and Protocols, experts and key figures within the field provide detailed protocols on leading approaches in the study of dopamine within biological systems. Divided into sections on cellular/biochemical, imaging, genetics, and electrophysiology, this collections includes protocols for bioluminescence and fluorescence imaging, receptor immunoprecipitation and proteomic analysis, creation and characterization of a mouse model of Parkinson’s disease, real time measurement of dopamine in the brain, and modeling signal transduction in silico.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and convenient, Dopamine: Methods and Protocols serves as an ideal guide for students and experts alike, as well as for anyone interested in exploring the vast and crucial field of dopamine research.

Content Level » Professional/practitioner

Keywords » Catecholamine - Cellular imaging - Dopamine - Electrophysiology - Genetic manipulation - Neurotransmitter - Physiological role

Related subjects » Neuroscience

Table of contents 

Part I: Biochemical, Proteomic, and Computational Tools


1. Detection of Cell Surface Dopamine Receptors

            Jiping Xiao and Clare Bergson


2. Methods for the Study of Dopamine Receptors Within Lipid Rafts of Kidney Cells

            Peiying Yu, Van Anthony Villar, and Pedro A. Jose


3. Methods of Dopamine Research in Retina Cells

            Ana Lucia Marques Ventura, Fernando Garcia de Mello, and Ricardo Augusto de Melo Reis


4. Capture of D2 Dopamine Receptor Signaling Complexes in Striatal Cells for Mass Spectrometry Proteomic Analysis

            Nadine Kabbani and Jacob C. Nordman


5. Modeling Spatial Aspects of Intracellular Dopamine Signaling

            Kim Blackwell, Lane J. Wallace, BoHung Kim, Rodrigo Oliveira, and Wonryull Koh


Part II: Cellular Imaging


6. A Biophysical Approach for the Study of Dopamine Receptor Oligomerization

            Sylwia Lukasiewicz, Agata Faron-Górecka, and Marta Dziedzicka-Wasylewska


7. Detection of Receptor Heteromers Involving Dopamine Receptors by the Sequential BRET-FRET (SRET) Technology

            Gemma Navarro, Peter J. McCormick, Josefa Mallol, Carme Lluís, Rafael Franco, Antoni Cortés, Vicent Casadó, Enric I. Canela, and Sergi Ferré


8. BRET Approaches to Characterize Dopamine and TAAR1 Receptor Pharmacology and Signaling

            Stefano Espinoza, Bernard Masri, Ali Salahpour, and Raul R. Gainetdinov


9. Dopaminergic Regulation of Dendritic Calcium: Fast Multi-Site Calcium Imaging

            Wen-Liang Zhou, Katerina D. Ikonomu, Shaina M. Short, and Srdjan D. Antic


Part III: Genetic Manipulation in Cells and Organisms


10. Functional Analysis of Human D1 and D5 Dopaminergic G Protein-Coupled Receptors: Lessons from Mutagenesis of a Conserved Serine Residue in the Cytosolic End of Transmembrane Region 6

            Bianca Plouffe and Mario Tiberi


11. A Molecular Genetic Approach to Uncovering the Differential Functions of Dopamine D2 Receptor Isoforms

            Yanyan Wang, Toshikuni Sasaoka, and Mai T. Dang


12. Genomic Strategies for the Identification of Dopamine Receptor Genes in Zebrafish

            Wendy Boehmler, Jessica Petko, Victor A. Canfield, and Robert Levenson


13. Application of Cell-Specific Isolation to the Study of Dopamine Signaling in Drosophila

            Eswar Prasad R. Iyer, Srividya Chandramouli Iyer, and Daniel N. Cox


Part IV: Electrochemical, Physiological, and Behavioral Analysis


14. Regulation of Dopamine Transporter Expression by Neuronal Activity

            Shalini Padmanabhan, Thach Pham, and Balakrishna M. Prasad


15. Monitoring Somatodendritic and Axonal Dopamine Release Using Fast-Scan Cyclic Voltammetry in Brain Slices

            Jyoti C. Patel and Margaret E. Rice


16. Real-Time Chemical Measurements of Dopamine Release in the Brain

            James G. Roberts, Leyda Z. Lugo-Morales, Philip L. Loziuk, and Leslie A. Sombers


17. The MPTP/Probenecid Model of Progressive Parkinson’s Disease

            Anna R. Carta, Ezio Carboni, and Saturnino Spiga

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