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Genetically Encoded Functional Indicators

  • Book
  • © 2012

Overview

Editors:
  1. Jean-René Martin

    1. ICFC, Neurobiologie & Developpement, CNRS, UPR-3294, Gif-sur-Yvette, France

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  • Presents a comprehensive overview of the biophysical and biochemical properties of the titular probes
  • Features easy and accessible descriptions of the main imaging tools and methods now available
  • Includes detailed protocols based on the most cutting-edge biological advances

Part of the book series: Neuromethods (NM, volume 72)

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Table of contents (9 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. In Vivo Functional Brain Imaging Using a Genetically Encoded Ca2+-Sensitive Bioluminescence Reporter, GFP-Aequorin

    • Jean-RenĂ© Martin
    Pages 1-26

  3. Ca2+ Imaging in Brain Slices Using Bioluminescent Reporters

    • Ludovic Tricoire, Estelle Drobac, Bertrand Lambolez
    Pages 27-42

  4. Calcium Imaging of Neural Activity in the Olfactory System of Drosophila

    • Antonia Strutz, Thomas Völler, Thomas Riemensperger, AndrĂ© Fiala, Silke Sachse
    Pages 43-70

  5. Functional Imaging of Antennal Lobe Neurons in Drosophila with Synapto-pHluorin

    • Dinghui Yu, Ronald L. Davis
    Pages 71-81

  6. Performing Electrophysiology and Two-Photon Calcium Imaging in the Adult Drosophila Central Brain During Walking Behavior

    • M. Eugenia Chiappe, Vivek Jayaraman
    Pages 83-101

  7. In Vivo Optical Recording of Brain Interneuron Activities from a Drosophila Male on a Treadmill

    • Soh Kohatsu, Masayuki Koganezawa, Daisuke Yamamoto
    Pages 103-112

  8. Two-Photon Imaging of Population Activity with Genetically Encoded Calcium Indicators in Living Flies

    • Robert A. A. Campbell, Kyle S. Honegger, Eyal Gruntman, Glenn C. Turner
    Pages 113-124

  9. Engineering and Application of Genetically Encoded Calcium Indicators

    • Jasper Akerboom, Lin Tian, Jonathan S. Marvin, Loren L. Looger
    Pages 125-147

  10. Imaging cAMP Dynamics in the Drosophila Brain with the Genetically Encoded Sensor Epac1-Camps

    • Katherine R. Lelito, Orie T. Shafer
    Pages 149-168

  11. Back Matter

    Pages 169-169
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Keywords

About this book

In the last decade, several different optical imaging techniques, either based on various voltage or calcium dyes, or more recently on modified fluorescent or bioluminescent proteins (genetically encoded) that are sensitive to calcium, have been developed to study neuronal activity, and especially groups of neurons, with the goal of mapping and deciphering the neural code underlying major neurophysiological functions.  Genetically Encoded Functional Indicators brings together expert contributors to present the development of recent genetic techniques that allow for generating genetically encoded activity sensors in order to investigate neuronal activity. Each chapter describes a specific sensor and its utilization to study neuronal activity in a particular way.  Written in the Neuromethods series style, chapters contain the kind of key description and implementation advice that guarantees successful results.

 

Helpful and easy to use, Genetically Encoded Functional Indicators aims to inspire students and researchers and to serve as a useful guide to those who wish to start using these different brain imaging techniques and require a bit of guidance in how best to choose a technique to match the goal of their study.

Editors and Affiliations

  • ICFC, Neurobiologie & Developpement, CNRS, UPR-3294, Gif-sur-Yvette, France

    Jean-René Martin

Bibliographic Information

  • Book Title: Genetically Encoded Functional Indicators

  • Editors: Jean-RenĂ© Martin

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-62703-014-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-62703-013-7Published: 01 August 2012

  • Softcover ISBN: 978-1-4939-5938-9Published: 23 August 2016

  • eBook ISBN: 978-1-62703-014-4Published: 01 August 2012

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XII, 169

  • Topics: Neurosciences, Human Genetics, Neurology

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