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Biomedical Sciences - Neuroscience | Neuropeptides - Methods and Protocols


Methods and Protocols

Series: Methods in Molecular Biology, Vol. 789

Merighi, Adalberto (Ed.)

2011, XI, 416p. 149 illus.. With online files/update.

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  • Features contributions from scientists with direct experience in one or more of the fields of neuropeptide research
  • Include detailed protocols relevant to a wide range of scientists
  • Provides key tips and expert implementation advice to ensure successful results

Since the early days, the field of neuropeptide biology has dramatically widened, and today the ultimate frontiers in neuropeptide research lie in the development of pharmacologically active compounds that are capable of crossing the blood-brain barrier to exert their biological role(s) in vivo and in the construction of genetic vectors to be employed in gene therapy.  Neuropeptides: Methods and Protocols presents a readily reproducible collection of established and emerging techniques for neuropeptide research as contributed by expert researchers in the field.  The detailed methods presented cover subjects such as immunocytochemical localization, biochemical characterization, functional analysis, development and production of genetic probes, and the design of neuropeptide derivatives for cellular neurobiology as well as the potential therapeutic applications.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and easy to follow, Neuropeptides: Methods and Protocols is intended for a large audience of scientists, including histologists, biochemists, cellular and molecular biologists, and electrophysiologists that are currently active in the field or are willing to enter this exciting and still expanding area of neurobiology.

Content Level » Professional/practitioner

Keywords » Cellular neurobiology - Chemical neuroanatomy - Functional analysis - Genetic probes - Immunocytochemical localization - Neurons - Protein molecules - Therapeutic applications

Related subjects » Neuroscience

Table of contents 

1. What Are Neuropeptides?

            J. Peter H. Burbach


2. Neuropeptide Localization in Non Mammalian Vertebrates

            Paolo de Girolamo and Carla Lucini


3. Combined Light and Electron Microscopic Visualization of Neuropeptides and Their Receptors in Central Neurons

            Chiara Salio, Laura Lossi, and Adalberto Merighi


4. Neuropeptide RNA Localization in Tissue Sections

            Marc Landry, Chérine Abdel Salam, and Marie Moftah


5. Intron-Specific Neuropeptide Probes

            Harold Gainer, Todd A. Ponzio, Chunmei Yue, and Makoto Kawasaki


6. Direct In Situ RT-PCR

            Laura Lossi, Graziana Gambino, Chiara Salio, and Adalberto Merighi


7. Laser Capture Microdissection and Quantitative-PCR Analysis

            Sarah J. Paulsen and Leif K. Larsen


8. Visualization of Peptide Secretory Vesicles in Living Nerve Cells

            Park J. Joshua and Loh Y. Peng


9. Fluorescence Imaging with Single-Molecule Sensitivity and Fluorescence Correlation Spectroscopy (FCS) of Cell-Penetrating Neuropeptides

            Vladana Vukojević, Astrid Gräslund, and Georgy Bakalkin


10. Analysis of Neuro-Immune Interactions by an In Vitro Co-Culture Approach

            Tadahide Furuno and Mamoru Nakanishi


11. Electrophysiology

            Zhi-Qing David Xu


12. Localization of Neuropeptides by Radioimmunoassay

            Fred Nyberg and Mathias Hallberg


13. Reversed-Phase HPLC and Hyphenated Analytical Strategies for Peptidomics

            Anne-Marie Hesse, Sega Ndiaye, and Joelle Vinh


14. Neuropeptidomics: Mass Spectrometry-Based Qualitative and Quantitative Analysis

            Ping Yin, Xiaowen Hou, Elena V. Romanova, and Jonathan V. Sweedler


15. Suppression Subtractive Hybridization

            Mohamed T. Ghorbel and David Murphy


16. Neuropeptide Microdialysis in Free-Moving Animals

            Tetsuya Kushikata and Kazuyoshi Hirota


17. Antibody Microprobes for Detecting Neuropeptide Release

            Carole A. Williams and Arthur W. Duggan


18. Neuropeptidases

            Manuel Ramírez, Isabel Prieto, Inmaculada Banegas, Ana B. Segarra, and Francisco Alba


19. Neuropeptide Autoantibodies Assay

            Sergueï O. Fetissov


20. Intranasal Delivery of Neuropeptides

            Michael C. Veronesi, Daniel J. Kubek, and Michael J. Kubek


21. Prodrug Design for Brain Delivery of Small- and Medium-Sized Neuropeptides

            Katalin Prokai-Tatrai and Laszlo Prokai


22. Measurement of Phosphorothioate Oligodeoxynucleotide Antisense Transport Across the Blood-Brain Barrier

            William A. Banks


23. Liposome-Encapsulated Neuropeptides for Site-Specific Microinjection

            Frédéric Frézard, Robson A.S. dos Santos, and Marco A.P. Fontes


24. Recombinant Adeno-Associated Viral Vectors

            Marijke W.A. de Backer, Keith M. Garner, Mieneke C.M. Luijendijk, and Roger A.H. Adan


25. De-Orphanizing G Protein-Coupled Receptors by a Calcium Mobilization Assay

            Isabel Beets, Marleen Lindemans, Tom Janssen, and Peter Verleyen

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