Logo - springer
Slogan - springer

Biomedical Sciences - Neuroscience | Methods of Glial Cell Culture

Methods of Glial Cell Culture

Boullerne, Anne, Osterhout, Donna

2015, 210 p. 150 illus., 60 illus. in color.

Available Formats:
eBook
Information

Springer eBooks may be purchased by end-customers only and are sold without copy protection (DRM free). Instead, all eBooks include personalized watermarks. This means you can read the Springer eBooks across numerous devices such as Laptops, eReaders, and tablets.

You can pay for Springer eBooks with Visa, Mastercard, American Express or Paypal.

After the purchase you can directly download the eBook file or read it online in our Springer eBook Reader. Furthermore your eBook will be stored in your MySpringer account. So you can always re-download your eBooks.

 

ISBN 978-1-4614-5924-8

digitally watermarked, no DRM

The eBook version of this title will be available soon


learn more about Springer eBooks

add to marked items

Hardcover
Information

Hardcover version

You can pay for Springer Books with Visa, Mastercard, American Express or Paypal.

Standard shipping is free of charge for individual customers.

 
approx. $99.00

(net) price for USA

ISBN 978-1-4614-5923-1

free shipping for individuals worldwide

Due: June 15, 2015


add to marked items

  • About this book

  • Outlines detailed methods for the isolation, purification and propagation of glial cells, such as oligodendrocytes, astrocytes, microglia and Schwann cells
  • Fills a major void in the methodology publications
  • Up-to-date research is valuable to both clinicians and scientists alike
Some glia function primarily as physical support for neurons. Others regulate the internal environment of the brain, especially the fluid surrounding neurons and their synapses, and provide nutrition to nerve cells. Glia have important developmental roles, guiding migration of neurons in early development and producing molecules that modify the growth of axons and dendrites. Recent findings in the hippocampus and cerebellum have indicated that glia are also active participants in synaptic transmission, regulating clearance of neurotransmitter from the synaptic cleft, releasing factors such as ATP which modulate presynaptic function, and even releasing neurotransmitters themselves. This book outlines detailed methods for the isolation, purification and propagation of glial cells, such as oligodendrocytes, astrocytes, microglia and Schwann cells. This fills a major void in the methodology publications, as most books on neural cell cultures are focused on neuronal cell culture. Their protocols for glial cultures are too succinct to be useful to a glial cell biologist, either novice or established. The purpose of this book is to provide clear and detailed protocols that are designed to be informative and easy to reproduce for the novice. One unique feature of this book is "trouble shooting" sections in each chapter to help solve the typical cell culture problems for each cell type.

Content Level » Research

Related subjects » Neuroscience

Table of contents 

Oligodendrocytes.- Astrocytes.- Microglia.- Schwann Cells.- Neuron-Glia Co-cultures.- Neurospheres.- Immortalization of Primary Cells

Popular Content within this publication 

 

Articles

Services for this book

New Book Alert

Get alerted on new Springer publications in the subject area of Neurosciences.