Techniques in Molecular Medicine

1. Front Matter

   Pages I-XII

   PDF

2. Molecular Biology Techniques

   1. Front Matter

      Pages 1-1

      PDF

   2. Structure and Function of the Human Genome

      • Friedhelm Hildebrandt

      Pages 3-23

   3. Enzymatic Modification of DNA and RNA

      • Kai-Olaf Netzer

      Pages 24-49

   4. Purification of Nucleic Acids From Eukaryotic Cells

      • Hans Gerd Nothwang, Friedhelm Hildebrandt

      Pages 50-70

   5. Electrophoresis of DNA and RNA Fragments

      • Feng Qian, Gregory G. Germino

      Pages 71-81

   6. Single-Strand Conformation Polymorphism (SSCP) Analysis

      • Feng Qian, Gregory G. Germino

      Pages 82-85

   7. Denaturing Gradient Gel Electrophoresis

      • Kai-Olaf Netzer

      Pages 86-95

   8. Introduction to Pulsed-Field Gel Electrophoresis (PFGE)

      • Feng Qian, Gregory G. Germino

      Pages 96-102

   9. Pulsed-Field Gel Electrophoresis: Protocols

      • Gudrun A. Rappold, Karin Ried, Albrecht Klink, Ercole Rao, Birgit Weiss

      Pages 103-113

   10. Radioactive Labeling of DNA and RNA Probes

       • Peter Igarashi

       Pages 114-125

   11. Hybridization Methods (Southern and Northern Blotting)

       • Kai-Olaf Netzer

       Pages 126-147

   12. Specimen Preparation Techniques for Cytogenetic Analysis by Fluorescence in situ Hybridization

       • Stefan Joos, Martin Bentz, Anton H. N. Hopman, Peter Lichter

       Pages 148-165

   13. Chromosome Analysis by Fluorescence in situ Hybridization

       • Martin Bentz, Stefan Joos, Peter Lichter

       Pages 166-185

   14. Sequencing

       • Klaus Deichmann

       Pages 186-206

   15. Polymerase Chain Reaction

       • Friedhelm Hildebrandt, Iva Singh-Sawhney

       Pages 207-225

   16. Cloning Vectors

       • Annette Deichmann, Klaus Deichmann

       Pages 226-254

3. Strategies for Gene Isolation and Characterization

   1. Front Matter

      Pages 255-255

      PDF

   2. Cloning Strategies: An Overview

      • Friedhelm Hildebrandt

      Pages 257-260

   3. Genomic Libraries

      • Luiz F. Onuchic, Gregory G. Germino

      Pages 261-272

Standard molecular biology techniques are used widely in biomedical re­ search and have had a major impact on the understanding of physiologic processes and disease mechanisms. They also play an increasing role in mo­ lecular genetic diagnosis and new therapeutic approaches. This laboratory manual is designed for researchers who need reliable pro­ tocols for their laboratorywork. It provides a step by step approach to stan­ dard methods of molecular biology. A wide range of topics is covered by protocols for immediate application in the laboratory. These include pur­ ification, isolation, labeling, hybridization, sequencing and enzymatic mod­ ification ofDNA or RNA, polymerase chain reaction, cloning, libraries, and protein techniques. The manual is based on experience with a practical course in molecular biologytechniques, which was taught bythe authors. Since most methods in molecular biology recapitulate in a test tube mechanisms that occur natu­ rally in the cell, the first three chapters describe and illustrate basic mechan­ isms of molecular biology. U nderstanding more about these basic mechan­ isms will enable the researcher to apply techniques more specifically, based on the knowledge of their potential and limits. W e hope that users find this manual a helpful guide on their road to exciting insights.

• Chromosomen
• DNA
• Elektrophorese
• Polymerasen
• RNA
• Restriction enzymes
• Restriktionsenzyme
• biology
• chromosome
• cloning
• enzyme
• hybridization
• molecular biology
• polymerases
• protein

• Universitäts-Kinderklinik, Freiburg, Germany

  Friedhelm Hildebrandt

• Section of Nephrology, Dep. of Internal Medicine, Yale University, New Haven, USA

  Peter Igarashi

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