Phosphorescent Oxygen-Sensitive Probes

1. Front Matter

   Pages i-viii

   PDF

2. O2-Sensitive Probes Based on Phosphorescent Metalloporphyrins

   • Ruslan I. Dmitriev, Dmitri B. Papkovsky

   Pages 1-28

3. O2 Analysis on a Fluorescence Spectrometer or Plate Reader

   • Alexander V. Zhdanov, James Hynes, Ruslan I. Dmitriev, Dmitri B. Papkovsky

   Pages 29-69

4. O2 Imaging in Biological Specimens

   • Andreas Fercher, Alexander V. Zhdanov, Dmitri B. Papkovsky

   Pages 71-101

Biological O₂ sensing probes and measurement techniques were first introduced in the late 80s. In the last 3-5 years they have undergone major development that have made them available and affordable for a broad range of applications in various disciplines of the life and biomedical sciences. These new chemistries and technologies, which are significantly different from the majority of other fluorescence-based probes and detection techniques, have already demonstrated their high utility. This book will provide a systematic overview of the existing and emerging O₂ sensing technologies in their different modifications, a practical guide to their rational selection and use, and examples of biological applications/case studies, including details on how to set up and conduct such experiments, troubleshoot and interpret the data.

• Hypoxia
• Oxygen imaging
• Oxygen sensitive probes
• Phosphorescence quenching
• Phosphorescent porphyrins
• Respiration and metabolism
• biochemical engineering

• Dept. Biochemistry, University College Cork, Cork, Ireland

  Dmitri B. Papkovsky

• , Biochemistry Department, University College Cork, Cork, Ireland

  Alexander V. Zhdanov, Andreas Fercher, Ruslan I. Dmitriev

• BioInnovation Centre, University College Cork, Cork, Ireland

  James Hynes

Dmitri B. Papkovsky is a Professor of Biochemistry, Head of Biophysics and Bioanalysis lab at the University College Cork, Ireland, where he has been working since 1997. His research interests include sensing of O2 in cells and tissues with phosphorescent probes, time-resolved fluorescence and bioimaging, the roles of oxygen in biological systems, O2 as a marker of mitochondrial and cellular function, metabolism, hypoxia research. Prof. Papkovsky is author of 100+ publications in peer-reviewed journals. He is one of the founders of an Irish company Luxcel Biosciences Ltd. specialising on these probes and applications. Dr. Alexander Zhdanov, Dr. Ruslan Dmitriev and Dr. Andreas Fercher are experienced postdoctoral researchers working in the lab of Prof. D. Papkovsky.

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