Logo - springer
Slogan - springer

Biomedical Sciences - Molecular Medicine | Wound Regeneration and Repair - Methods and Protocols

Wound Regeneration and Repair

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1037

Gourdie, Robert G., Myers, Tereance A. (Eds.)

2013, XVIII, 585 p. 122 illus., 102 illus. in color.

A product of Humana Press
Available Formats:

Springer eBooks may be purchased by end-customers only and are sold without copy protection (DRM free). Instead, all eBooks include personalized watermarks. This means you can read the Springer eBooks across numerous devices such as Laptops, eReaders, and tablets.

You can pay for Springer eBooks with Visa, Mastercard, American Express or Paypal.

After the purchase you can directly download the eBook file or read it online in our Springer eBook Reader. Furthermore your eBook will be stored in your MySpringer account. So you can always re-download your eBooks.


(net) price for USA

ISBN 978-1-62703-505-7

digitally watermarked, no DRM

Included Format: PDF and EPUB

download immediately after purchase

learn more about Springer eBooks

add to marked items


Hardcover version

You can pay for Springer Books with Visa, Mastercard, American Express or Paypal.

Standard shipping is free of charge for individual customers.


(net) price for USA

ISBN 978-1-62703-504-0

free shipping for individuals worldwide

usually dispatched within 3 to 5 business days

add to marked items

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

In Wound Regeneration and Repair: Methods and Protocols, expert researchers in the field detail classical and cutting-edge methods for studying wound healing and regeneration. These techniques include cellular and molecular methods, genetic approaches, surgical procedures, clinical advances, drug discovery and delivery modalities, animal and humanized models and new applications in the treatment of pathological wounds in a variety of organs and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, a detailed list of the necessary materials and reagents for each procedure, step-by-step, reproducible laboratory protocols, and a set notes, developed by the authors, for troubleshooting and avoiding known pitfalls.



Authoritative and practical, Wound Regeneration and Repair: Methods and Protocols seeks to aid scientists and entrepreneurs in their further study of technologies, models, techniques, and critical new areas and approaches to clinical and commercial translation of research.

Content Level » Professional/practitioner

Keywords » cardiovascular models of injury repair - clinical advances - drug discovery - genetic approaches - humanized models - non-mammalian models - organs - pathological wounds - skin - stem cell based approaches to wound healing - surgical techniques - tissue engineering - tissues - wounds

Related subjects » Molecular Medicine

Table of contents 

Part I: Tissue Engineered Wound Repair Models

1. An In Vivo Model System for Evaluation of the Host Response to Biomaterials                        

Brian Sicari, Neill Turner, and Stephen Badylak


2. Urothelial Cell Culture

Yuanyuan Zhang and Anthony Atala


3. Cell Populated Collagen Lattices Contraction Model for the

Investigation of Fibroblast Collagen Interactions

H Paul Ehrlich, and Kurtis E Moyer,


4. A Tissue-engineered Corneal Wound Healing Model for the Characterization of Reepithelialization

Karine Zaniolo, Patrick Carrier, Sylvain L. Guérin, François A. Auger and Lucie Germain



Part II: Stem Cells and Would Repair

5. Adult Stem Cells in Small Animal Wound Healing Models

Nauta, Allison C., Gurtner, Geoffrey C., Longaker, and  Michael T.,


6. Novel Animal Models for Tracking the Fate and Contributions of Bone Marrow Derived Cells in Diabetic Healing

Robert C. Caskey and Kenneth W. Liechty




8. Cell-Based Therapies for Myocardial Repair: Emerging Role for Bone Marrow Derived Mesencymal Stem Cells (MSCs) in the Treatment of the Chronically Injured Heart

Jose S. Da Silva and Joshua M. Hare


9. A Model System for Primary Abdominal Closures

Michael J. Yost, Mary O. Morales, Veronica Rodriguez-Rivera, Eric M. Yost, Louis Terracio and Stephen A. Fann


Part III: Skin and Corneal Wound Repair Models

10.  Alternatives for Animal Wound Model Systems

Phil Stephens, Matthew Caley, and Matthew Peake


11. Novel Methods for the Investigation of Human Hypertrophic Scarring and other Dermal Fibrosis

Dariush Honardoust, Peter Kwan, Moein Momtazi, Jie Ding , and Edward E Tredget



12. Study of the Human Chronic Wound Tissue: Addressing Logistic Barriers and Productive use of Laser Capture Microdissection

Sashwati Roy and Chandan K Sen



13. The Wound Watch: An Objective Staging System for Wounds in the Diabetic (db/db) Mouse Model

G. Pietramaggiori, , S. Scherer, and  DP. Orgill,


14. Human Ex vivo Wound Healing Model

Stojadinovic Olivera and Marjana Tomic – Canic


15. Murine Models of Human Wound Healing

Jerry S. Chen, Michael T. Longaker, and Geoffrey C. Gurtner



Part IV: Cardiovascular Wound Healing Models

16. A Corneal Scarring Model

Daniel J. Gibson and Gregory S. Schultz


17. A Novel and Efficient Model of Coronary Artery Ligation in the Mouse

Erhe Gao and Walter J. Koch


18. Cardiac Wound Healing Post-Myocardial Infarction: A Novel Method to Target Extracellular Matrix Remodeling in the Left Ventricle

Rogelio Zamilpa, Jianhua Zhang, Ying Ann Chiao, Lisandra de Castro Bras, Ganesh Halade, Yonggang Ma,  Sander O. Hacker, and Merry L. Lindsey


19. Injury Models to Study Cardiac Remodeling in the Mouse: Myocardial Infarction and Ischemia-reperfusion

Daniel J. Luther, Charles K. Thodeti, and J. Gary Meszaros


20. Cryoinjury Models of the Adult and Neonatal Mouse Heart for Studies of Scarring and Regeneration

Erik G. Strungs, Emily L. Ongstad, Michael P. O’Quinn, Joseph A. Palatinus, L. Jane Jourdan, and Robert G. Gourdie


21. Targeting Wnt Signaling to Improve Wound Healing after Myocardial Infarction

Evangelos P. Daskalopoulos, Ben J.A. Janssen and W. Matthijs Blankesteijn


22. Vascular Connexins in Restenosis after Balloon Injury

Sandrine Morel and Brenda R. Kwak



Part V: Non Mammalian and Mammalian Models of Regenerative Repair

23. Gain-of-function Assays in the Axolotl (Ambystoma mexicanum) to Identify Signaling Pathways that Induce and Regulate Limb Regeneration

Jangwoo Lee, Cristian Aguilar and David Gardiner


24. The Mouse Digit Tip: from Wound Healing to Regeneration

Jennifer Simkin, Manjong Han, Ling Yu, Mingquan Yan, and Ken Muneoka

 25. Lower Vibrissa Follicle Amputation: A Mammalian Model of RegenerationJames M. Waters and Allison J. Cowin


26. Using Drosophila Larvae to Study Epidermal Wound Closure and Inflammation

Sirisha Burra, Yan Wang, Amanda R. Brock, and Michael J. Galko


27.  Zebrafish Cardiac Injury and Regeneration Models: A Non-invasive and Invasive in vivo Model of Cardiac Regeneration

Michael S. Dickover, Ruilin Zhang, Peidong Han, and Neil C. Chi



Part VI: Wound Healing Cell Biology

28. Quantifying Alterations in Cell Migration: Tracking Fluorescently Tagged Migrating Cells by FACs and Live Imaging

Rachael Z. Murray


29. Examining the Role of Mast Cells in Fetal Wound Healing using Cultured Cells in vitro

Brian C. Wulff and Traci A. Wilgus


30. Assessing Macrophage Phenotype During Tissue Repair

Timothy J. Koh, Margaret L. Novak, and Rita E. Mirza


31. The use of Connexin-Based Therapeutic Approaches to Target Inflammatory Diseases

Simon J O’Carroll, David L Becker, Joanne O Davidson, Alistair J Gunn, Louise FB Nicholson, and  Colin R Green


Part VII: Commercialization of Wound Repair and Regenerative Discoveries

32. Commercialization: Patenting and Licensing in Wound Healing and Regenerative Biology

W.Chip. Hood and David Huizenga

 33. Translational Strategies for the Development of a Wound Healing Technology (idea) from Bench to Bedside

Gautam S. Ghatnekar  and Tuan A. Elstrom


Popular Content within this publication 



Read this Book on Springerlink

Services for this book

New Book Alert

Get alerted on new Springer publications in the subject area of Molecular Medicine.