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  • © 2012

Liver Proteomics

Methods and Protocols

  • Outlines strategies used to characterize liver proteome at the global, cellular, subcellular, post transitional, and functional level
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 909)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xiii
  2. Cell Surface Capturing Technologies for the Surfaceome Discovery of Hepatocytes

    • Damaris Bausch-Fluck, Andreas Hofmann, Bernd Wollscheid
    Pages 1-16
  3. An On-Target Desalting and Concentration Sample Preparation Protocol for MALDI-MS and MS/MS Analysis

    • Xumin Zhang, Quanhui Wang, Xiaomin Lou, Haidan Sun, Peter Roepstorff, Siqi Liu
    Pages 17-28
  4. Plasma Membrane Isolation Using Immobilized Concanavalin A Magnetic Beads

    • Yu-Chen Lee, Martina Srajer Gajdosik, Djuro Josic, Sue-Hwa Lin
    Pages 29-41
  5. Producing a One-Dimensional Proteomic Map for Human Liver Cytochromes P450

    • Natalia A. Petushkova, Andrey V. Lisitsa
    Pages 63-82
  6. Assessing Heterogeneity of Peroxisomes: Isolation of Two Subpopulations from Rat Liver

    • Markus Islinger, Afsaneh Abdolzade-Bavil, Sven Liebler, Gerhardt Weber, Alfred Völkl
    Pages 83-96
  7. Purification and Proteomic Analysis of Liver Membrane Skeletons

    • Jintang He, Yashu Liu, Qingsong Wang, Jianguo Ji
    Pages 97-111
  8. Quantitative Analysis of Liver Golgi Proteome in the Cell Cycle

    • Xuequn Chen, Philip C. Andrews, Yanzhuang Wang
    Pages 125-140
  9. Phosphoproteomic Analysis of Liver Homogenates

    • Gokhan Demirkan, Arthur R. Salomon, Philip A. Gruppuso
    Pages 151-163
  10. A Combination of Affinity Chromatography, 2D DIGE, and Mass Spectrometry to Analyze the Phosphoproteome of Liver Progenitor Cells

    • Enrique Santamaría, Virginia Sánchez-Quiles, Joaquín Fernández-Irigoyen, Fernando J. Corrales
    Pages 165-180
  11. Identifying Acetylated Proteins in Mitosis

    • Carol Chuang, Li-yuan Yu-Lee
    Pages 181-204
  12. Proteomics Analysis of Human Nonalcoholic Fatty Liver

    • Eva Rodriguez-Suarez, Jose M. Mato, Felix Elortza
    Pages 241-258
  13. Proteomic Methods for Biomarker Discovery in a Rat Model of Alcohol Steatosis

    • Billy W. Newton, William K. Russell, David H. Russell, Shashi K. Ramaiah, Arul Jayaraman
    Pages 259-277

About this book

The liver is responsible for a wide range of critical functions essential to life, and is composed of several different cell types. In  Liver Proteomics: Methods and Protocols, expert researchers in the field detail many of the methods that are used to study the live. These methods include the  most up-to-date strategies being used to characterize the liver proteome at the global, cellular, subcellular, post translational and functional level.Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Liver Proteomics: Methods and Protocols seeks to aid scientists in the further study of this crucially important organ.

Editors and Affiliations

  • Warren Alpert Medical School, Proteomics Core Facility, Brown University, Providence, USA

    Djuro Josic

  • Div. Hematology & Oncology, Rhode Island Hospital, Providence, USA

    Douglas C. Hixson

Bibliographic Information

  • Book Title: Liver Proteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Djuro Josic, Douglas C. Hixson

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-959-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2012

  • Hardcover ISBN: 978-1-61779-958-7Published: 18 August 2012

  • Softcover ISBN: 978-1-4939-6214-3Published: 23 August 2016

  • eBook ISBN: 978-1-61779-959-4Published: 18 August 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 346

  • Topics: Molecular Medicine, Proteomics

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access