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Liver Proteomics

Methods and Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Djuro Josic

    1. Warren Alpert Medical School, Proteomics Core Facility, Brown University, Providence, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Douglas C. Hixson

    1. Div. Hematology & Oncology, Rhode Island Hospital, Providence, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Outlines strategies used to characterize liver proteome at the global, cellular, subcellular, post transitional, and functional level
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 909)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Cell Surface Capturing Technologies for the Surfaceome Discovery of Hepatocytes

    • Damaris Bausch-Fluck, Andreas Hofmann, Bernd Wollscheid
    Pages 1-16

  3. An On-Target Desalting and Concentration Sample Preparation Protocol for MALDI-MS and MS/MS Analysis

    • Xumin Zhang, Quanhui Wang, Xiaomin Lou, Haidan Sun, Peter Roepstorff, Siqi Liu
    Pages 17-28

  4. Plasma Membrane Isolation Using Immobilized Concanavalin A Magnetic Beads

    • Yu-Chen Lee, Martina Srajer Gajdosik, Djuro Josic, Sue-Hwa Lin
    Pages 29-41

  5. Analysis of the Cattle Liver Proteome by High-Sensitive Liquid Chromatography Coupled with Mass Spectrometry Method

    • Anna Maria Timperio, Gian Maria D’Amici, Lello Zolla
    Pages 43-62

  6. Producing a One-Dimensional Proteomic Map for Human Liver Cytochromes P450

    • Natalia A. Petushkova, Andrey V. Lisitsa
    Pages 63-82

  7. Assessing Heterogeneity of Peroxisomes: Isolation of Two Subpopulations from Rat Liver

    • Markus Islinger, Afsaneh Abdolzade-Bavil, Sven Liebler, Gerhardt Weber, Alfred Völkl
    Pages 83-96

  8. Purification and Proteomic Analysis of Liver Membrane Skeletons

    • Jintang He, Yashu Liu, Qingsong Wang, Jianguo Ji
    Pages 97-111

  9. Liver Plasma Membranes: An Effective Method to Analyze Membrane Proteome

    • Rui Cao, Songping Liang
    Pages 113-123

  10. Quantitative Analysis of Liver Golgi Proteome in the Cell Cycle

    • Xuequn Chen, Philip C. Andrews, Yanzhuang Wang
    Pages 125-140

  11. Glycoproteins and Glycosylation: Apolipoprotein C3 Glycoforms by Top-Down MALDI-TOF Mass Spectrometry

    • Yan Zhang, Alan R. Sinaiko, Gary L. Nelsestuen
    Pages 141-150

  12. Phosphoproteomic Analysis of Liver Homogenates

    • Gokhan Demirkan, Arthur R. Salomon, Philip A. Gruppuso
    Pages 151-163

  13. A Combination of Affinity Chromatography, 2D DIGE, and Mass Spectrometry to Analyze the Phosphoproteome of Liver Progenitor Cells

    • Enrique Santamaría, Virginia Sánchez-Quiles, Joaquín Fernández-Irigoyen, Fernando J. Corrales
    Pages 165-180

  14. Identifying Acetylated Proteins in Mitosis

    • Carol Chuang, Li-yuan Yu-Lee
    Pages 181-204

  15. Comparative Proteome Analysis of a Human Liver Cell Line Stably Transfected with Hepatitis D Virus Full-Length cDNA

    • Celso Cunha, Ana V. Coelho
    Pages 205-225

  16. Identification of New Autoimmune Hepatitis-Specific Autoantigens by Using Protein Microarray Technology

    • Lin Wu, Guang Song
    Pages 227-239

  17. Proteomics Analysis of Human Nonalcoholic Fatty Liver

    • Eva Rodriguez-Suarez, Jose M. Mato, Felix Elortza
    Pages 241-258

  18. Proteomic Methods for Biomarker Discovery in a Rat Model of Alcohol Steatosis

    • Billy W. Newton, William K. Russell, David H. Russell, Shashi K. Ramaiah, Arul Jayaraman
    Pages 259-277

  19. Targeted Mass Spectrometry-Based Metabolomic Profiling Through Multiple Reaction Monitoring of Liver and Other Biological Matrices

    • Angelo D’Alessandro, Federica Gevi, Lello Zolla
    Pages 279-294

  20. Discovery of Lamin B1 and Vimentin as Circulating Biomarkers for Early Hepatocellular Carcinoma

    • Kwong-Fai Wong, John M. Luk
    Pages 295-310
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Keywords

About this book

The liver is responsible for a wide range of critical functions essential to life, and is composed of several different cell types. In  Liver Proteomics: Methods and Protocols, expert researchers in the field detail many of the methods that are used to study the live. These methods include the  most up-to-date strategies being used to characterize the liver proteome at the global, cellular, subcellular, post translational and functional level.Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Liver Proteomics: Methods and Protocols seeks to aid scientists in the further study of this crucially important organ.

Editors and Affiliations

  • Warren Alpert Medical School, Proteomics Core Facility, Brown University, Providence, USA

    Djuro Josic

  • Div. Hematology & Oncology, Rhode Island Hospital, Providence, USA

    Douglas C. Hixson

Bibliographic Information

  • Book Title: Liver Proteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Djuro Josic, Douglas C. Hixson

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-959-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2012

  • Hardcover ISBN: 978-1-61779-958-7Published: 18 August 2012

  • Softcover ISBN: 978-1-4939-6214-3Published: 23 August 2016

  • eBook ISBN: 978-1-61779-959-4Published: 18 August 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 346

  • Topics: Molecular Medicine, Proteomics

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