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Therapeutic Antibodies

Methods and Protocols

  • Book
  • © 2009

Overview

Editors:
  1. Antony S. Dimitrov
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  • Outlines the current state and future trends for therapeutic antibodies
  • Provides complete guidelines to discover and develop therapeutic antibodies, from antigen production to animal studies
  • Presents a modular approach with each part of the book tracing a stage of antibody discovery and preclinical development
  • Details the most recent protocols in antibody discovery and engineering, currently used by leading academic laboratories and biotechnology companies
  • Describes alternate approaches for antibody discovery and characterization
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 525)

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Table of contents (32 protocols)

  1. Front Matter

    Pages i-xviii
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  2. Recombinant Antigens

    1. Front Matter

      Pages 1-1
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    2. Therapeutic Antibodies: Current State and Future Trends – Is a Paradigm Change Coming Soon?

      • Dimiter S. Dimitrov, James D. Marks
      Pages 1-27

    3. Preparation of Recombinant Viral Glycoproteins for Novel and Therapeutic Antibody Discovery

      • Yee-Peng Chan, Lianying Yan, Yan-Ru Feng, Christopher C. Broder
      Pages 31-58

  3. Antibody Libraries

    1. Front Matter

      Pages 1-1
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    2. Design of a Human Synthetic Combinatorial Library of Single-Chain Antibodies

      • Limor Nahary, Itai Benhar
      Pages 61-80

    3. Construction of a Human Antibody Domain (VH) Library

      • Weizao Chen, Zhongyu Zhu, Xiaodong Xiao, Dimiter S. Dimitrov
      Pages 81-99

    4. Generation and Selection of Rabbit Antibody Libraries by Phage Display

      • Christoph Rader
      Pages 101-128

    5. Construction of a Large Naïve Human Phage-Displayed Fab Library Through One-Step Cloning

      • Zhongyu Zhu, Dimiter S. Dimitrov
      Pages 129-142

  4. Antibody Discovery

    1. Front Matter

      Pages 1-1
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    2. Identification of Target and Function Specific Antibodies for Effective Drug Delivery

      • Yu Zhou, James D. Marks
      Pages 145-160

    3. Screening of Specific Internalization Fab Fragment from Human Naive Phage Library by Combinational Bio-Panning

      • Xin Wang, Brian B. Cao
      Pages 161-174

    4. Competitive Antigen Panning for Selection of HIV-1 Neutralizing Human Monoclonal Antibodies Specific for gp41

      • Mei-Yun Zhang, Dimiter S. Dimitrov
      Pages 175-186

    5. Selection of Non-aggregating VH Binders from Synthetic VH Phage-Display Libraries

      • Mehdi Arbabi-Ghahroudi, Roger MacKenzie, Jamshid Tanha
      Pages 187-216

    6. Isolation of Full-Length IgG Antibodies from Combinatorial Libraries Expressed in Escherichia coli

      • Yariv Mazor, Thomas Van Blarcom, Brent L. Iverson, George Georgiou
      Pages 217-239

    7. Multiplexed Flow Cytometry: High-Throughput Screening of Single-Chain Antibodies

      • Joanne Ayriss, Rosa Valero, Andrew R.M. Bradbury, Peter Pavlik
      Pages 241-260

    8. Human Antibody Repertoires

      • Per-Johan Meijer, Lars S. Nielsen, Johan Lantto, Allan Jensen
      Pages 261-277

    9. Rapid Screening Platform for Stabilization of scFvs in Escherichia coli

      • Brian R. Miller, Scott M. Glaser, Stephen J. Demarest
      Pages 279-289

  5. Antibody Engineering

    1. Front Matter

      Pages 1-1
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    2. In Vitro Antibody Affinity Maturation Targeting Germline Hotspots

      • Mitchell Ho, Ira Pastan
      Pages 293-308
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Keywords

About this book

Over 2000 years ago in China, antibodies elicited by early forms of vaccination likely played a major role in the protection of the population from infectious agents. Vac- nation has been further developed in Europe and described by Edward Jenner in the late-eighteenth century, then successfully implemented worldwide. The idea to use theactiveingredientinthebloodofvaccinated(orimmunized)animalsorhumansfor the treatment of diseases came a century later. It was made possible by a series of discoveries,suchastherealizationthattheserumfromanimalsimmunizedwithtoxins, for example, diphtheria toxin or viruses, is an effective therapeutic against the disease causedbythesameagentinhumans. Inthe1880s,vonBehringdevelopedanantitoxin (anti-body) that did not kill the bacteria but neutralized the bacterial toxin. The first Nobel Prize in Medicine (1901) was given to him for the discovery of the serum therapy. Acenturylater,22monoclonalantibodies(mAbs)areapprovedbytheUnited States Food and Drug Administration (FDA) for clinical use, and hundreds are in clinicaltrialsforthetreatmentofvariousdiseasesincludingcancers,immunedisorders, and infections. The revenues from the top-five therapeutic antibodies reached $11. 7 billion in 2006, and major pharmaceutical companies raced to acquire antibody biotech companies with a recent example of MedImmune, Inc. , which was acquired for $15. 6 billion by AstraZeneca in 2007. This explosion of research and development in the field of therapeutic antibodies prompted the publication of the MiMB volume Therapeutic Antibodies: Methods and Protocols. The book’s major goal is to present a set of protocols useful for researchers discoveringanddevelopingtherapeuticantibodies. Currentadvancesandfuturetrends in the antibody therapeutics are analyzed in thelead-in review article.

Reviews

From the reviews:

"This is an informative and practical handbook for those involved in the discovery and development of therapeutic antibodies. … Intended for those in the pharmaceutical industry or academia … and in the offices of those who direct projects which include this type of work. … This is a valuable addition to the libraries of those who perform day-to-day work in this field, or who manage projects involving therapeutic antibodies. … this is a valuable guide." (Frederick D. Coffman, Doody’s Review Service, June, 2009)

Bibliographic Information

  • Book Title: Therapeutic Antibodies

  • Book Subtitle: Methods and Protocols

  • Editors: Antony S. Dimitrov

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-554-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-934115-92-3Published: 05 March 2009

  • Softcover ISBN: 978-1-62703-869-0Published: 25 November 2014

  • eBook ISBN: 978-1-59745-554-1Published: 28 February 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVIII, 585

  • Topics: Immunology, Antibodies

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