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Biomedical Sciences - Immunology | Monoclonal Antibodies - Methods and Protocols

Monoclonal Antibodies

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1131

Ossipow, Vincent, Fischer, Nicolas (Eds.)

2nd ed. 2014, XIV, 575 p. 108 illus., 27 illus. in color.

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  • Offers modern approachse to generate high quality monoclonals
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Monoclonal Antibodies: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed modern approaches to isolate and characterize monoclonal antibodies against carefully selected epitopes. This edition includes new chapters covering the key steps to generate high quality monoclonals via different methods, from antigen generation to epitope mapping and quality control of the purified IgG. Chapters are divided into four parts corresponding to four distinct objectives. Part I covers monoclonal antibody generation, Part II deals with monoclonal antibody expression and purification, Part III presents methods for monoclonal antibody characterization and modification, and Part IV describes selected applications of monoclonal antibodies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Monoclonal Antibodies: Methods and Protocols, Second Edition provides crucial initial steps of monoclonal antibody generation and characterization with state-of-the art protocols.

Content Level » Professional/practitioner

Keywords » aggregated monoclonals - antigen generation - crystallization chaperoning - downstream purification of IgG - immunofluorescence - monoclonal antibodies - monoclonal antibody characterization - monoclonal antibody expression - monoclonal antibody generation - monoclonal antibody modification - monoclonal antibody purification - mutagenesis - phage- or bacterial display - solid-state arrays - unstable monoclonals

Related subjects » Biomedical Sciences - Immunology

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