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Biomedical Sciences - Immunology | Mouse Models of Allergic Disease - Methods and Protocols

Mouse Models of Allergic Disease

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1032

Allen, Irving C. (Ed.)

2013, XI, 326 p. 51 illus., 27 illus. in color.

A product of Humana Press
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  • Serves as an essential collection of protocols from leading authors in a variety of disciplines
  • Features detailed, lab-ready methodologies
  • Includes expert tips and key implementation advice to ensure successful results

Over the last half century, a dramatic increase in allergic diseases has been observed throughout industrialized nations, which has resulted in significant worldwide socio-economic challenges. In Mouse Models of Allergic Disease: Methods and Protocols, a wide range of expert contributors provide detailed protocols for the design and execution of experiments to thoroughly analyze critical elements associated with a diverse range of allergic diseases, all through the lens of mouse models that accurately recapitulate clinically relevant aspects of the respective human disease.  The volume opens with a section featuring techniques essential for effective ex vivo cell isolation and evaluation of specific cell types relevant to a diverse range of allergic diseases, and the book then moves on to cover in vivo protocols to evaluate prevalent mouse models of human allergic diseases, including mouse models of systemic anaphylaxis, contact hypersensitivity, allergic rhinitis, and asthma, as well as a collection of chapters on in vivo and ex vivo protocols used to assess indirect mediators of allergic diseases, such as the nervous system, non-hematopoietic cells, and the composition of the gut microbiome.  Written in the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Timely and authoritative, Mouse Models of Allergic Disease: Methods and Protocols serves as an essential collection of protocols that allow both novice and expert researchers the ability to accurately develop, evaluate, and characterize the mechanisms associated with these disorders.

Content Level » Professional/practitioner

Keywords » Allergic rhinitis - Allergy - Asthma - Contact hypersensitivity - Human allergic diseases - In vivo and Ex vivo protocols - Indirect mediators of allergies - Mouse models - Systemic anaphylaxis

Related subjects » Immunology

Table of contents 

1. Transgenic Mouse Models

            Jennifer Skelton, Linda H. Gower, Anne McGough, and Patricia A. Labosky


2. Pulmonary Antigen Presenting Cells: Isolation, Purification, and Culture

            Hideki Nakano and Donald N. Cook


3. Evaluation of T Cell Function in Allergic Disease

            Brianne R. Barker


4. Evaluating B-Cells: From Bone Marrow Precursors to Antibody Producing Cells

            M. Manuela Rosado, Marco Scarsella, Simona Cascioli, Ezio Giorda, and Rita Carsetti


5. Protocols for Identifying, Enumerating and Assessing Mouse Eosinophils

            Kimberly D. Dyer, Katia E. Garcia-Crespo, Caroline M. Percopo, Eva M. Sturm, and Helene F. Rosenberg


6. Evaluation of Classical, Alternative, and Regulatory Functions of Bone Marrow-Derived Macrophages

            Beckley K. Davis


7. Applications of Mouse Airway Epithelial Cell Culture for Asthma Research

            Amjad Horani, John D. Dickinson, and Steven L. Brody


8. Isolation and Characterization of Mast Cells in Mouse Models of Allergic Diseases

            Martina Kovarova


9. Purifying and Measuring Immunoglobulin E (IgE) and Anti-IgE

            Jamie L. Sturgill and Daniel H. Conrad


10. Protocols for the Induction and Evaluation of Systemic Anaphylaxis in Mice

            Elizabeth Doyle, Julia Trosien, and Martin Metz


11. Contact Hypersensitivity Models in Mice

            Irving C. Allen


12. Induction of Allergic Rhinitis in Mice

            Virginia McMillan Carr and Alan M. Robinson


13. Induction of Allergic Airway Disease Using House Dust Mite Allergen

            Irving C. Allen


14. An Inhalation Model of Allergic Fungal Asthma: Aspergillus fumigatus-Induced Inflammation and Remodeling in Allergic Airways Disease

            Jane M. Schuh and Scott A. Hoselton


15. PAMPs and DAMPs in Allergy Exacerbation Models

            Monique A.M. Willart, Philippe Poulliot, Bart N. Lambrecht, and Mirjam Kool


16. Assessment of Airway Hyperresponsiveness in Mouse Models of Allergic Lung Disease Using Detailed Measurements of Respiratory Mechanics

            John M. Hartneyand Annette Robichaud


17. Bilateral Vagotomy as a Tool for Determining Autonomic Involvement in Airway Responses in Mouse Models of Asthma

            Jaime M. Cyphert


18. Clara Epithelial Cell Depletion in the Lung

            Sanchaita S. Sonar and Jan C. Dudda


19. A Mouse Model for Evaluating the Contribution of Fibrocytes and Myofibroblasts to Airway Remodeling in Allergic Asthma

            Matthias Schmidt and Sabrina Mattoli


20. Assessment of Airway Hyperresponsiveness in Murine Tracheal Rings

            Jeremiah T. Herlihy, Iurii Semenov, and Robert Brenner


21. Use of the Cockroach Antigen Model of Acute Asthma to Determine the Immunomodulatory Role of Early Exposure to Gastrointestinal Infection

            Carolyn G. Durham, Lisa M. Schwiebert, and Robin G. Lorenz


22. Expression Profiling to Identify Candidate Genes Associated with Allergic Phenotypes

            Willie June Brickey


23. Flow Cytometric Methods for the Assessment of Allergic Disease

            Adeeb H. Rahman


24. Generation of Bone Marrow and Fetal Liver Chimeric Mice

            Eda K. Holl

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