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Biomedical Sciences - Immunology | Mouse Models of Innate Immunity - Methods and Protocols

Mouse Models of Innate Immunity

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1031

Allen, Irving C. (Ed.)

2013, XI, 225 p. 27 illus., 20 illus. in color.

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  • Includes detailed contributions from a diverse group of authors
  • Features both ex vivo and in vivo protocols that are lab-ready
  • Contains expert tips and key implementation advice to ensure successful results

The innate immune system represents a critical arm of the immune response by providing immediate and robust host defense; however, human studies of its function are often limited by ethical, logistical, and technical obstacles. In Mouse Models of Innate Immunity: Methods and Protocols, experts in the field explore the design and execution of experiments used to thoroughly evaluate critical elements associated with the host innate immune response. The volume opens with methods that are essential for collecting and assessing various primary cells that are highly relevant to innate immunity, and it continues with in vivo protocols commonly used to evaluate the innate immune response in the mouse, including mouse models of respiratory infection, gastrointestinal inflammation, fungal and parasitic diseases, sepsis, and HIV-1 infection.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and easy to use, Mouse Models of Innate Immunity: Methods and Protocols will serve the research community by providing expert advice and protocols that allow both experienced and novice investigators to successfully plan, implement, and assess disease processes associated with the innate immune response.

Content Level » Professional/practitioner

Keywords » Disease process assessment - Host innate immune response - Mouse genetics - Mouse models - Primary cells

Related subjects » Immunology

Table of contents 

1. Conventional Murine Gene Targeting

            Albert G. Zimmermann and Yue Sun

 

2. Production and Characterization of Humanized Rag2-/-γc-/- Mice

            Freddy M. Sanchez, German I. Cuadra, Stanton J. Nielsen, Anne Tanner, and Bradford K. Berges

 

3. Isolation, Culture, and Functional Evaluation of Bone Marrow-Derived Macrophages

            Beckley K. Davis

 

4. Collecting Resident or Thioglycollate Elicited Peritoneal Macrophages

            Monika Schneider

 

5. Quantification and Visualization of Neutrophil Extracellular Traps (NETs) from Murine Bone Marrow-Derived Neutrophils

            Linda Vong, Philip M Sherman, and Michael Glogauer

 

6. Assessment of Oxidative Metabolism

            Emilie Imbeault and Denis Gris

 

7. Generation and Culture of Mouse Embryonic Fibroblasts

            Yu Lei

 

8. Primary Ear Fibroblast Derivation from Mice

            Chris B. Moore and Irving C. Allen

 

9. Bone Marrow Derived Dendritic Cells

            Kelly Roney

 

10. Measuring T Cell Function in Innate Immune Models

            Brianne R. Barker

 

11. Bioassay for the Measurement of Type-I Interferon Activity

            Douglas G. Widman

 

12. Safe and Effective Mouse Foot-Pad Inoculation

            Kristin M. Long and Mark Heise

 

13. Delayed Type Hypersensitivity Models in Mice

            Irving C. Allen

 

14. Mouse Model of Staphylococcus aureus Skin Infection

            Natalia Malachowa, Scott D. Kobayashi, Kevin R. Braughton, and Frank R. DeLeo

 

15. Sepsis Induced by Cecal Ligation and Puncture

            Haitao Wen

 

16. Systemic Infection of Mice with Listeria monocytogenes to Characterize Host Immune Responses

            Nancy Wang, Richard A. Strugnell, Odilia L. Wijburg, and Thomas C. Brodnicki

 

17. Mouse Model of Invasive Fungal Infection

            Donna M. MacCallum

 

18. Endotoxin-Induced Uveitis in Rodents

            Umesh C.S. Yadav and Kota V. Ramana

 

19. Bacteria-Mediated Acute Lung Inflammation

            Irving C. Allen

 

20. Intranasal Influenza Infection of Mice and Methods to Evaluate Progression and Outcome

            Catherine J. Sanders, Brian Johnson, Charles W. Frevert, and Paul G. Thomas

 

21. Dextran Sodium Sulfate-Induced Murine Inflammatory Colitis Model

            Monika Schneider

 

22. Bacterial-Mediated Gastrointestinal Inflammation

            Joshua Uronis and Xiaolun Sun

 

23. Plasmodium berghei ANKA (PbA) Infection of C57BL/6J Mice: A Model of Severe Malaria

            Marcela Montes de Oca and Ashraful Haque

 

24. Characterization of HIV-1 Infection in the Humanized Rag2-/-γc-/- Mouse Model

            Freddy M. Sanchez and Bradford K. Berges

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