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Antigen Processing

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Peter Endert

    1. Institut National de la Santé et de la Recherche Médicale, Unité 1013, Université Paris Descartes, Faculté de médecine, Paris, France

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  • Details comprehensive methods to study antigen presentation by classical and non-classical MHC proteins
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 960)

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Table of contents (43 protocols)

  1. Front Matter

    Pages i-xvii
    Download chapter PDF

  2. Purification of Large Cytosolic Proteases for In Vitro Assays: 20S and 26S Proteasomes

    • Stefan Tenzer, Tobias Hain, Hendrik Berger, Hansjörg Schild
    Pages 1-14

  3. Analysis of Proteasome Generated Antigenic Peptides by Mass Spectrometry

    • Kathrin Textoris-Taube, Christin Keller, Ulrike Kuckelkorn, Peter-M. Kloetzel
    Pages 15-29

  4. Using Protease Inhibitors in Antigen Presentation Assays

    • Michael Basler, Marcus Groettrup
    Pages 31-39

  5. Kinetic Studies of Cytoplasmic Antigen Processing and Production of MHC Class I Ligands

    • Anita Stargardt, Eric Reits
    Pages 41-51

  6. Assaying Peptide Translocation by the Peptide Transporter TAP

    • Marlieke L. M. Jongsma, Jacques Neefjes
    Pages 53-65

  7. In Vitro Reconstitution of the MHC Class I Peptide-Loading Complex

    • Pamela A. Wearsch, Peter Cresswell
    Pages 67-79

  8. Studying MHC Class I Peptide Loading and Exchange In vitro

    • Marlene Bouvier
    Pages 81-91

  9. Measuring Synthesis and Degradation of MHC Class I Molecules

    • David B. Guiliano, Antony N. Antoniou
    Pages 93-108

  10. Studying Ubiquitination of MHC Class I Molecules

    • Marian L. Burr, Jessica M. Boname, Paul J. Lehner
    Pages 109-125

  11. Evaluation of Viral Interference with MHC Class I-Restricted Antigen Processing and Presentation Using a Flow Cytometry-Based Approach

    • Daniëlle Horst, Maaike E. Ressing, Arend Mulder, Emmanuel J. H. J. Wiertz
    Pages 127-136

  12. Determining the Activity of the Transporter Associated with Antigen Processing in the Compartments of the Secretory Pathway

    • Esther Ghanem, Sebastian Springer
    Pages 137-144

  13. Biochemical Large-Scale Identification of MHC Class I Ligands

    • Daniel J. Kowalewski, Stefan Stevanović
    Pages 145-157

  14. Establishing MHC Class I Peptide Motifs

    • Nico Trautwein, Stefan Stevanović
    Pages 159-168

  15. Quantitating MHC Class I Ligand Production and Presentation Using TCR-Like Antibodies

    • Brian P. Dolan
    Pages 169-177

  16. Biochemical Analysis of Naturally Processed Antigenic Peptides Presented by MHC Class I Molecules

    • Takayuki Kanaseki, Nilabh Shastri
    Pages 179-185

  17. Identifying Source Proteins for MHC Class I-Presented Peptides

    • Nathalie Vigneron, Wenbin Ma, Alexandre Michaux, Benoît J. Van den Eynde
    Pages 187-207

  18. Purification, Preparation, and Use of Chaperone–Peptide Complexes for Tumor Immunotherapy

    • Ayesha Murshid, Jianlin Gong, Stuart K. Calderwood
    Pages 209-217

  19. Recombinant Poxviruses: Versatile Tools for Immunological Assays

    • Nicholas A. Siciliano, Lan Huang, Laurence C. Eisenlohr
    Pages 219-245

  20. Bioinformatics Identification of Antigenic Peptide: Predicting the Specificity of Major MHC Class I and II Pathway Players

    • Ole Lund, Edita Karosiene, Claus Lundegaard, Mette Voldby Larsen, Morten Nielsen
    Pages 247-260
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Keywords

About this book

Antigen processing is a biological process that prepares antigens for the presentation to special cells in the immune system called T lymphocytes. In Antigen Processing: Methods and Protocols, expert researchers in the field provide a comprehensive set of protocols for studying presentation of antigens produced in the standard processing pathways for MHC class I and class II molecules. The chapters follow chronology of intracellular processing events, ending with recognition of peptide-MHC complexes at the cell surface by T lymphocytes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Antigen Processing: Methods and Protocols is designed for beginners and experts interested in studying antigen processing.

Reviews

From the reviews:

“The book is divided into 42 chapters, each split into a short abstract, an introduction providing the relevant bibliographic overview, a detailed list of materials used in the protocol, a step-by-step description of the procedure, and a final section with notes. … Van Endert achieves what he set out to do – to provide an in-depth bench-side companion for antigen processing beginners and experts … . The book comes highly recommended.” (Clemens Hermann, Immunology News, February, 2014)

“The book is designed to be a benchside companion for researchers interested in studying antigen processing. It is intended for both beginners and experts studying antigen presentation.” (Omer Iqbal, Doody’s Book Reviews, August, 2013)

Editors and Affiliations

  • Institut National de la Santé et de la Recherche Médicale, Unité 1013, Université Paris Descartes, Faculté de médecine, Paris, France

    Peter Endert

Bibliographic Information

  • Book Title: Antigen Processing

  • Book Subtitle: Methods and Protocols

  • Editors: Peter Endert

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-218-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-217-9Published: 18 January 2013

  • Softcover ISBN: 978-1-4939-5956-3Published: 23 August 2016

  • eBook ISBN: 978-1-62703-218-6Published: 19 January 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVII, 589

  • Number of Illustrations: 60 b/w illustrations, 25 illustrations in colour

  • Topics: Immunology, Antibodies

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