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Details comprehensive methods to study antigen presentation by classical and non-classical MHC proteins
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Antigen processing is a biological process that prepares antigens for the presentation to special cells in the immune system called T lymphocytes. In Antigen Processing: Methods and Protocols, expert researchers in the field provide a comprehensive set of protocols for studying presentation of antigens produced in the standard processing pathways for MHC class I and class II molecules. The chapters follow chronology of intracellular processing events, ending with recognition of peptide-MHC complexes at the cell surface by T lymphocytes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Antigen Processing: Methods and Protocols is designed for beginners and experts interested in studying antigen processing.
Content Level »Professional/practitioner
Keywords »MHC class I molecules - MHC class II molecules - cytosolic proteases - dendritic cells - endogenous peptides - endosomal proteases - kinetics of peptide - lentiviruses - macroautophagy - peptide-MHC