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Biomedical Sciences - Immunology | Single Domain Antibodies - Methods and Protocols

Single Domain Antibodies

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 911

Saerens, Dirk, Muyldermans, Serge (Eds.)

2012, XV, 580 p. 89 illus., 35 illus. in color.

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  • Features methods of selection, expression, improvement, and library construction of single domain antibodies
  • Includes step-by-step instruction that has been tested in labs around the world
  • Provides key tips and implementation advice that is essential for success
The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications; however, the bulky size of mAbs, costly production, and cumbersome engineering hampered regularly their streamlined development in some applications.  In Single Domain Antibodies: Methods and Protocols, expert researchers examine single variable domain antibody fragments, referred to as VH, VL, VHH or VNAR. These fragments are the smallest intact antigen-binding fragments that can be produced recombinantly at low cost.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Content Level » Professional/practitioner

Keywords » Heavy chain antibodies - Single variable domain - V-NAR - VHH - sdAbs

Related subjects » Immunology

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