Vaccine Protocols

1. Front Matter

   Pages i-xi

   PDF

2. Overview of Vaccines

   • Gordon Ada

   Pages 1-17

3. Temperature-Sensitive Mutant Vaccines

   • Craig R. Pringle

   Pages 19-36

4. Live Viral Vectors

   • Anthony R. Fooks

   Pages 37-49

5. Live Viral Vectors

   • Caroline Staib, Gerd Sutter

   Pages 51-68

6. Live Viral Vectors

   • Gunilla B. Karlsson, Peter Liljeström

   Pages 69-81

7. Development of Attenuated Salmonella Strains That Express Heterologous Antigens

   • Frances Bowe, Derek J. Pickard, Richard J. Anderson, Patricia Londoño-Arcila, Gordon Dougan

   Pages 83-100

8. Expression and Delivery of Heterologous Antigens Using Lactic Acid Bacteria

   • Mark A. Reuter, Sean Hanniffy, Jerry M. Wells

   Pages 101-114

9. Synthetic Peptides

   • Michael J. Francis

   Pages 115-131

10. Genetic Detoxification of Bacterial Toxins

    • Mariagrazia Pizza, Maria Rita Fontana, Vincenzo Scarlato, Rino Rappuoli

    Pages 133-151

11. Preparation of Polysaccharide-Conjugate Vaccines

    • Carla C. A. M. Peeters, Patrick R. Lagerman, Odo de Weers, Lukas A. Oomen, Peter Hoogerhout, Michel Beurret et al.

    Pages 153-173

12. Adjuvant Formulations for Experimental Vaccines

    • Duncan E. S. Stewart-Tull

    Pages 175-193

13. Incorporation of Immunomodulators into Plasmid DNA Vaccines

    • Linda S. Klavinskis, Philip Hobson, Andrew Woods

    Pages 195-210

14. Microencapsulation of Vaccine Antigens

    • David H. Jones

    Pages 211-222

15. Lyophilization of Vaccines

    • Gerald D. J. Adams

    Pages 223-243

16. Stimulation of Mucosal Immunity

    • David J. M. Lewis, Christopher M. M. Hayward

    Pages 245-253

17. Induction and Detection of T-Cell Responses

    • Kingston H. G. Mills

    Pages 255-277

18. Construction of MHC Class I-Peptide Tetrameric Complexes for Analysis of T-Cell-Mediated Immune Responses

    • Rachel V. Samuel, Tomáš Hanke

    Pages 279-287

19. Assessment of Functional Antibody Responses

    • Ray Borrow, Paul Balmer

    Pages 289-300

20. The Use of Complete Genome Sequences in Vaccine Design

    • Nigel J. Saunders, Sarah Butcher

    Pages 301-312

Vaccine research and development is advancing at an unprecedented pace, with an increasing emphasis on rational design based upon a fundamental und- standing of the underlying molecular mechanisms. The aim of this volume is to provide a selection of contemporary protocols that will be useful to both novice and advanced practitioner alike. The variety of procedures required to design, develop, produce, and assess a vaccine is immense and covers aspects of ch- istry, biochemistry, molecular biology, cell biology, and immunology. No single volume can hope to cover these topics exclusively. Rather, here we attempt to provide a methods sourcebook focusing on hands-on practical advice. Comp- mentary and background information may be found in other volumes in the Me- ods in Molecular Medicine series. Of particular interest are volumes on Dendritic Cell Protocols, Interleukin Protocols, Vaccine Adjuvants, and DNA Vaccines. Since the publication of the first edition of Vaccine Protocols there have been major advances, particularly in the areas of bacterial genomics, antig- specific T-cell quantification, genetic manipulation of vaccine vectors, the h- nessing of natural molecules concerned with the regulation of immune responses, and the burgeoning field of DNA vaccinology. Hence, the extensive revision of this edition with new chapters on live viral vaccine vectors, atte- ated bacterial vectors, immunomodulators, MHC-peptide tetrameric complexes, and the identification of vaccine candidates by genomic analysis. Additionally, chapters from the first edition have been updated to accommodate state-of-t- art methods in vaccinology.

"This manual serves as a valuable guide to the process of designing, producing and assessing vaccines used against infectious agents including viruses, bacteria and parasites. This book would most benefit scientists involved in the research and development associated with vaccine technology. It also would be most useful to those new to the field of vaccine formulation and production. Health and science libraries supporting research institutions, government agencies, academic and corporate entities involved in developing or producing vaccines will find this a valuable addition to the collection." -E-STREAMS

From reviews of the first edition...

"...cover[s] concepts and protocols applicable to a wide range of vaccines and infectious diseases and cancer...brings together many protocols of value to research and development scientists involved in the vaccine technology area."
-Immunology and Cell Biology

"...a valuable basic reference manual of vaccine related laboratory procedures...strongest in the area of vaccine development and delivery technology...especially valuable to those new to the field of vaccine development and formulation."
-Doody's Health Sciences Book Review Journal

• Centre for Applied Microbiology and Research, Health Protection Agency, Salisbury, UK

  Andrew Robinson, Michael J. Hudson

• Department of Cellular and Molecular Medicine, St. George’s Hospital Medical School, London, UK

  Martin P. Cranage

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