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Biomedical Sciences - Immunology | Antimicrobial Peptides - Methods and Protocols

Antimicrobial Peptides

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 618

Giuliani, Andrea, Rinaldi, Andrea C. (Eds.)

2010, XV, 424 p. 3 illus. in color.

A product of Humana Press
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  • Includes contributions from scientists from different disciplines and backgrounds, from both industry and academia
  • Provides notes sections describing major sources of problems that may occur with a particular technique and how they can be identified and overcome
  • Describes techniques and methods that can be applied beyond the specific field of antimicrobial peptide research

The action of antimicrobial peptides (AMPs), ranging from direct killing of invading pathogens to immune response modulation and other complex biological responses, has stimulated research and clinical interest for more than two decades, but the area is still burgeoning due to emerging discoveries in the functions, roles, and regulation of AMPs, thus making the study of antimicrobial peptides a multi-disciplinary and rapidly evolving field. In Antimicrobial Peptides: Methods and Protocols, leading investigators present a broad, up-to-date collection of current research and experimental methods for the isolation, characterization, production, and optimization of antimicrobial peptides. Additional chapters detail methodologies in several microscopy techniques, high-throughput screening, QSAR modeling, and computer-aided design used to study these compounds, while key review articles survey potential medical applications of antimicrobial peptides as innovative anti-infective and immunomodulatory agents, as well as emerging discoveries in their function, regulation, and roles in innate immunity. As a volume in the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and wide-ranging in its applications, Antimicrobial Peptides: Methods and Protocols provides both an authoritative guide for lab work on AMPs or related substances and a useful collection of thought stimuli to inspire further scientific endeavours in a wide array of vital fields.

Content Level » Professional/practitioner

Keywords » Escherichia coli - antibiotics - bacteria - biology - electron microscopy - immune response - immune system - immunity - infection - innate immunity - membrane - microscopy - molecular biology - peptides - research

Related subjects » Immunology

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