Originally published as volume 64 in the series: Methods in Molecular Medicine
2nd ed. 2010
A product of Humana Press
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Provides step-by-step procedures for DC isolation from rodent and human tissue, as well as easy in vitro generation of equivalent cell types
Explores diverse assays to measure DC function in the context of tolerance, autoimmune disease, allergy, and immunity to viral and bacterial infection
All assays used currently by leading experts in the field for high impact studies
Offers a digestible introduction to DC function and development, covering both humans and rodents
Includes techniques that are cutting edge and state-of-the-art in the field of DC biology
Contains a comprehensive list of models to test the effect that your favourite gene or immunological model has on DC development or function
Helps readers to generate a particular specialized DC subtype in vitro saving time with greater focus on a biologically relevant system
Examines isolating DC from almost all organs of the mouse
Aids in the identification of the myriad subtypes in human and mice for further study
Provides a standardized model for the use of human DCs in clinical trials
Given the vital importance of immune system research, the gathering of clear, consistent, and informative protocols involving the study of dendritic cells is paramount. Bringing the popular first edition fully up to date, Dendritic Cell Protocols, Second Edition presents protocols from experts in the field that cover the basics and more complex forays into the exploration of DC development and function, both in mice and humans. The first section of the volume involving humans explores topics such as the isolation of blood DC subtypes, primary skin Langerhans cells, and the generation of gene-manipulated human DCs with the inclusion of more clinically relevant methods as well, while the second section involving rodent models delves into DC and precursor generation in vitro, isolation ex vivo, disease models, as well as DC functions and properties. Written in the highly successful Methods in Molecular Biology™ series style, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Dendritic Cell Protocols, Second Edition aims to become a bench-side handbook for both beginners and experts in the field of DC research and a long-term reference for some of the most popular methods put forward by those who lead the field.