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Immunocytochemical Methods and Protocols

  • Book
  • © 2010

Overview

Editors:
  1. Constance Oliver

    1. Fac. Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil

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    You can also search for this editor in PubMed   Google Scholar

  2. Maria Célia Jamur

    1. Fac. Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Fully updated from the first edition to offer cutting-edge methods in immunocytochemistry
  • Includes methods applicable for the basic research laboratory as well as the clinical laboratory
  • Provides the theoretical basis behind the methods
  • Contains methods that have multidisciplinary applications
  • Suitable for beginning and experienced investigators

Part of the book series: Methods in Molecular Biology (MIMB, volume 588)

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Table of contents (43 protocols)

  1. Front Matter

    Pages i-xii
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  2. Antibody Preparation

    1. Front Matter

      Pages 1-1
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    2. Overview of Antibodies for Immunochemistry

      • Constance Oliver, Maria Célia Jamur
      Pages 3-9

    3. Introduction to the Purification of Antibodies

      • Ana Cristina Grodzki, Elsa Berenstein
      Pages 11-13

    4. Antibody Purification: Ammonium Sulfate Fractionation or Gel Filtration

      • Ana Cristina Grodzki, Elsa Berenstein
      Pages 15-26

    5. Antibody Purification: Ion-Exchange Chromatography

      • Ana Cristina Grodzki, Elsa Berenstein
      Pages 27-32

    6. Antibody Purification: Affinity Chromatography – Protein A and Protein G Sepharose

      • Ana Cristina Grodzki, Elsa Berenstein
      Pages 33-41

    7. Conjugation of Fluorochromes to Antibodies

      • Su-Yau Mao, J. Michael Mullins
      Pages 43-48

    8. Biotinylation of Antibodies

      • Su-Yau Mao
      Pages 49-52

  3. Tissue Preparation for Light Microscopic Analysis

    1. Front Matter

      Pages 54-54
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    2. Cell Fixatives for Immunostaining

      • Maria Célia Jamur, Constance Oliver
      Pages 55-61

    3. Permeabilization of Cell Membranes

      • Maria Célia Jamur, Constance Oliver
      Pages 63-66

    4. Preparation of Frozen Sections for Analysis

      • Gary L. Bratthauer
      Pages 67-73

    5. Processing of Cytological Specimens

      • Gary L. Bratthauer
      Pages 75-84

    6. Processing of Tissue Culture Cells

      • Gary L. Bratthauer
      Pages 85-92

    7. Processing of Tissue Specimens

      • Gary L. Bratthauer
      Pages 93-102

    8. Heat-Induced Antigen Retrieval for Immunohistochemical Reactions in Routinely Processed Paraffin Sections

      • Laszlo Krenacs, Tibor Krenacs, Eva Stelkovics, Mark Raffeld
      Pages 103-119

  4. Bright Field Detection Systems

    1. Front Matter

      Pages 122-122
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    2. Fluorochromes: Properties and Characteristics

      • J. Michael Mullins
      Pages 123-134

    3. Direct Immunofluorescent Labeling of Cells

      • Maria Veronica Dávila Pástor
      Pages 135-142
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Keywords

About this book

Antibodies tagged with fuorescent markers have been used in histochemistry for over 50 years. Although early applications were focused on the detection of microbial antigens in tissues, the use of immunocytochemical methods now has spread to include the det- tion of a wide array of antigens including proteins, carbohydrates, and lipids from virtually any organism. Today, immunohistochemistry is widely used to identify, in situ, various components of cells and tissues in both normal and pathological conditions. The method gains its strength from the extremely sensitive interaction of a specifc antibody with its antigen. For some scientifc areas, books have been published on applications of immu- cytochemical techniques specifc to that area. What distinguished Immunocytochemical Methods and Protocols from earlier books when it was frst published was its broad appeal to investigators across all disciplines, including those in both research and clinical settings. The methods and protocols p- sented in the frst edition were designed to be general in their application; the accompa- ing “Notes” provided the reader with invaluable assistance in adapting or troubleshooting the protocols. These strengths continued to hold true for the second edition and again for the third edition. Since the publication of the frst edition, the application of immuno- tochemical techniques in the clinical laboratory has continued to rise and this third edition provides methods that are applicable to basic research as well as to the clinical laboratory.

Reviews

From the reviews of the third edition:

“This book targets not only immunologists but a very broad readership. … very well-written and will be equally useful for a complete beginner to the technique, as well as for a scientist who has been using the methods for years. … I would recommend this book in particular to researchers who start to use immunocyto/histochemistry in their work … . The book should also be considered by laboratories that would like to have a reference for reliable immunostaining protocols.” (Anna Vossenkaemper, Immunology News, August, 2010)

Editors and Affiliations

  • Fac. Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil

    Constance Oliver, Maria Célia Jamur

Bibliographic Information

  • Book Title: Immunocytochemical Methods and Protocols

  • Editors: Constance Oliver, Maria Célia Jamur

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-324-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2010

  • Hardcover ISBN: 978-1-58829-463-0Published: 23 December 2009

  • Softcover ISBN: 978-1-61779-682-1Published: 06 April 2012

  • eBook ISBN: 978-1-59745-324-0Published: 12 December 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XII, 416

  • Number of Illustrations: 40 b/w illustrations, 4 illustrations in colour

  • Topics: Immunology, Pathology

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