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Biomedical Sciences - Immunology | Immunocytochemical Methods and Protocols

Immunocytochemical Methods and Protocols

Series: Methods in Molecular Biology, Vol. 588

Oliver, Constance, Jamur, Maria Célia (Eds.)

3rd ed. 2010

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  • Fully updated from previous editions to offer cutting-edge methods in immunocytochemistry
  • Includes methods applicable to the basic research laboratory as well as the clinical laboratory
  • Provides the theoretical basis to the methods
  • Contains methods that have multidisciplinary applications
  • Suitable for both novice and experienced investigators

The landscape of immunocytochemistry has significantly evolved in past years. Once a laborious technique with limited application, the introduction of new detection systems and instrumentation has facilitated the widespread use of immunocytochemical methods. In the post-genomic era, the use of immunocytochemical methods has grown to include the identification of a broad spectrum of antigens including proteins, carbohydrates and lipids from virtually every organism. In Immunocytochemical Methods and Protocols, Third Edition, expert researchers explore the modern methods employed in the field today,  now widely used to identify, in situ, various components of cells and tissues in both normal and pathological conditions, and to demonstrate the localization of subcellular components. Chapters provide methods that are designed to be general in their application and to appeal to investigators across all disciplines, including those in research and clinical settings. Composed in the highly successful Methods in Molecular Biology™ series format, chapters contain a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

 Comprehensive and ground-breaking,  Immunocytochemical Methods and Protocols, Third Edition, brings together a variety of methods that are normally limited to applications in a specific area, providing a unique and vital guide for researchers and clinicians alike.

Content Level » Professional/practitioner

Keywords » Antibody - Antigen - Fluorescence microscopy - IgG - Immunohistochemistry - Immunostaining

Related subjects » Immunology - Pathology

Table of contents 

Immunocytochemical Methods and Protocols, Third Edition Constance Oliver and Maria Célia Jamur, Eds. Table of Contents Preface Contributors Part I. Antibody Preparation Overview of Antibodies for Immunochemistry Constance Oliver and Maria Célia Jamur Introduction to the Purification of Antibodies Ana Cristina Grodzki and Elsa Berenstein Antibody Purification: Ammonium Sulfate Fractionation or Gel Filtration Ana Cristina Grodzki and Elsa Berenstein Antibody Purification: Ion - Exchange chromatography Ana Cristina Grodzki and Elsa Berenstein Antibody Purification: Affinity Chromatography - Protein A and Protein G Sepharose Ana Cristina Grodzki and Elsa Berenstein Conjugation of Fluorochromes to Antibodies Su-Yau Mao Biotinylation of Antibodies Su-Yau Mao Part II. Tissue Preparation for Light Microscopic Analysis Cell Fixatives for Immunostaining Maria Célia Jamur and Constance Oliver Permeablization of Cell Membranes Maria Célia Jamur and Constance Oliver Preparation of Frozen Sections for Analysis Gary L. Bratthauer Processing of Cytological Specimens Gary L. Bratthauer Processing of Tissue Culture Cells Gary L. Bratthauer Processing Of Tissue Specimens Gary L. Bratthauer Heat Induced Antigen Retrieval for Immunohistochemical Reactions in Routinely Processed Paraffin Sections Laszlo Krenacs, Tibor Krenacs, Eva Stekovics,and Mark Raffeld Part III: Bright Field Detection Systems Fluorochromes - Properties and Characteristics J. Michael Mullins Direct Immunofluorescent Labeling of Cells Maria Veronica Dávila Pástor Fluorescence Labeling of Surface Antigens of Attached or Suspended Tissue-Culture Cells Mark C. Willingham Fluorescence Labeling of Intracellular Antigens of Attached or Suspended Tissue-Culture Cells Mark C. Willingham Fluorescent Visualization of Macromolecules in Drosophila Whole Mounts Ricardo Guelerman Pinheiro Ramos, Luciana Claudia Herculano Machado, and Livia Maria Rosatto Moda Overview of Conventional Fluorescence Photomicrography J. Michael Mullins Overview of Confocal Microscopy William D. Swaim Overview of Laser Microbeam Applications as Related to Antibody Targeting P. Scott Pine Immuno-Laser Capture Microdissection of Rat Brain Neurons For Real Time Quantitative PCR L. Scot Bastian and Denis G. Baskin Overview of Antigen Detection through Enzymatic Activity Gary L. Bratthauer The Peroxidase-antiperoxidase (PAP) Method and Other All-Immunologic Detection Methods Gary L. Bratthauer The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods Gary L. Bratthauer Avidin-Biotin Labeling of Cellular Antigens in Cryostat-Sectioned Tissue Mark Raffeld and Elaine S. Jaffe Multiple Antigen Immunostaining Procedures Tibor Krenacs, Laszlo Krenacs and Mark Raffeld Immunoenzymatic Quantitative Analysis of Antigens

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