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Biomedical Sciences - Immunology | Basophils and Mast Cells - Methods and Protocols

Basophils and Mast Cells

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1192

Gibbs, Bernhard F., Falcone, Franco H. (Eds.)

2014, XI, 251 p. 39 illus., 28 illus. in color.

A product of Humana Press
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  • Includes cutting-edge methods and protocols involving mast cells and basophils
  • Provides step-by-step details essential for reproducible results
  • Contains key notes and implementation advice from the experts

In Basophils and Mast Cells: Methods and Protocols, experts in this challenging field explore techniques to research these cells from the most practical point of view. Given the tremendous influence of mast cells and blood-borne basophils over immune system function, this volume intends to aid the reader in the development of better tools for the isolation of these cells from primary tissues, peripheral blood, bone marrow, or cord blood. Also covered are protocols for the in vitro and in vivo study of their functions. Written in the highly successful Methods in Molecular Biology format, chapters in this book contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls straight from the researchers who use the methods most.

Authoritative and easy to use, Basophils and Mast Cells: Methods and Protocols will provide the necessary tools for future research into mast cells and basophils with the goal of aiding in the quest to shed more light on these fascinating cell types.

Content Level » Professional/practitioner

Keywords » Allergic inflammation - Anti-allergic therapies - Basophil purification - Immune system - Mast cell functions - Signaling pathways

Related subjects » Immunology

Table of contents 

Part I: Introduction & Historical Background


1. Paradigm Shifts in Mast Cell and Basophil Biology and Function: An Emerging View of Immune Regulation in Health and Disease

            Ana Olivera and Juan Rivera


Part II: Obtaining the Cells: Purification, Culture, Counting, and Cell Lines


2. Purification of Basophils from Peripheral Human Blood

            Franco H. Falcone and Bernhard F. Gibbs


3. Mast Cell Purification Protocols

            Jasmine Farrington, Elizabeth P. Seward, and Peter T. Peachell


4. Generation of a Human Allergic Mast Cell Phenotype from CD133+ Stem Cells

            Hans Jürgen Hoffmann


5. Generation of Mast Cells from Murine Stem Cell Progenitors

            Emily J. Swindle


6. Integration of the Human Dermal Mast Cell into the Organotypic Coculture Skin Model

            Jonghui Kim, Sven Guhl, Magda Babina, Torsten Zuberbier, and Metin Artuc


7. The Absolute Basophil Count

            Elena Borzova and Clemens A. Dahinden


8.  Detection of Mast Cells and Basophils by Immunohistochemistry

            Andrew F. Walls and Cornelia Amalinei


9. Mast Cell and Basophil Cell Lines: A Compendium

            Egle Passante


Part III: Whole Cells: Identification in Tissues, Signaling Pathways, Gene Silencing, and Reporters


10. Measuring Histamine and Cytokine Release from Basophils and Mast Cells

            Bettina M. Jensen, Sidsel Falkencrone, and Per S. Skov


11. Flow Cytometric Allergy Diagnosis: Basophil Activation Techniques

            Chris H. Bridts, Vito Sabato, Christel Mertens, Margo M. Hagendorens, Luc S. De Clerck, and Didier G. Ebo


12. Microscopy Assays for Evaluation of Mast Cell Migration and Chemotaxis

            Monika Bambousková, Zuzana Hájková, Pavel Dráber, and Petr Dráber


13. Use of Humanized Rat Basophil Leukaemia (RBL) Reporter Systems for Detection of Allergen-Specific IgE Sensitization in Human Serum

            Daniel Wan, Xiaowei Wang, Ryosuke Nakamura, Marcos J.C. Alcocer, and Franco H. Falcone


14. Gene Silencing Approaches in Mast Cells and Primary Human Basophils

            Vadim V. Sumbayev and Bernhard F. Gibbs


15. Basophil Stimulation and Signaling Pathways

            Edward F. Knol and Bernhard F. Gibbs


16. Identification and Immunophenotypic Characterization of Normal and Pathological Mast Cells

            José Mário Morgado, Laura Sánchez-Muñoz, Cristina Teodósio, and Luís Escribano


Part IV: Whole Organism: Disease and Knockout Models


17. Identification of Murine Basophils by Flow Cytometry and Histology

            Christian Schwartz and David Voehringer


18. Mast Cell Mediated Reactions In Vivo

            Vladimir Andrey Giménez-Rivera, Martin Metz, and Frank Siebenhaar

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