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Biomedical Sciences - Immunology | Regulatory B Cells - Methods and Protocols

Regulatory B Cells

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1190

Vitale, Gaetano, Mion, Francesca (Eds.)

2014, XI, 292 p. 53 illus., 19 illus. in color.

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  • Includes cutting-edge methods and protocols involving B cells with suppressive functions and their immunological properties
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Regulatory B Cells: Methods and Protocols presents the current experimental set-ups and methodologies useful for the identification and characterization of B cells with suppressive functions and for the study of their biological and immunological properties. Organized into four sections, this detailed volume covers the basic methods for the isolation and immunophenotypical characterization of specific B cell subsets from mouse and human tissues, methods for the investigation of the mechanisms of immune suppression operated by B cells, several experimental approaches for the ex vivo generation/expansion of IL-10-producing B cells, as well as procedures for the study of the immune suppressive function of B cells in specific pathological settings. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Practical and authoritative, Regulatory B Cells: Methods and Protocols serves as an ideal guide for immunologists as well as for cell and molecular biologists interested in the intricacies of B cell biology.

Content Level » Professional/practitioner

Keywords » Anti-inflammatory cytokines - Breg - IL-10 - Immune regulation - Immune suppression - Pathophysiology - Suppressive function

Related subjects » Cell Biology - Immunology

Table of contents 

Part I: Purification and Phenotypic Characterization of B Cell Subsets


1. Purification and Immunophenotypic Characterization of Murine MZ and T2-MZP Cells

            M. Manuela Rosado, Marco Scarsella, Simona Cascioli, Ezio Giorda, and Rita Carsetti


2. Purification and Immune-Phenotyping of B-1 Cells from Body Cavities of Mice

            Vanessa Yenson and Nicole Baumgarth


3. Purification and Immunophenotypic Characterization of Murine B10 B Cells

            Chao Hong and Xiao-Ming Gao


4. Purification and Immunophenotypic Characterization of Human B Cells with Regulatory Functions

            Jean-David Bouaziz, Hélène Le Buanec, and Adèle de Masson


Part II: Mechanisms of Immune Suppression by B Cells


5. IL-10 Detection in Murine B Cells: Pros and Cons of the Different Techniques

            Jun Deng, Xiang Lin, and Liwei Lu


6. TGF-β Detection and Measurement in Murine B Cells: Pros and Cons of the Different Techniques

            Yoshiyuki Mishima, Shunji Ishihara, Jonathan J. Hansen, and Yoshikazu Kinoshita


7. Characterization and Activity of Fas Ligand Producing CD5+ B Cells

            Steven K. Lundy and Matthew W. Klinker


Part III: Methods for the Ex Vivo Generation/Expansion of IL-10-Producing B Cells


8. Utilization of a Lentiviral System for the Generation of B Cells with Regulatory Properties

            Elisabeth Calderón-Gómez and Simon Fillatreau


9. Ex Vivo Generation of Murine IL-10-Producing B Cells by Fusokines

            Aurélie Tormo, Jiusheng Deng, Edouard Al-Chami, Ziouani Sonia, and Moutih Rafei


10. Toll-Like Receptor Ligation for the Induction of Regulatory B Cells

            Luciën E.P.M van der Vlugt, Simone Haeberlein, Wouter de Graaf, Tyrill E.D Martha, and Hermelijn H. Smits


11. The Generation of Regulatory B Cells by Helminth Parasites

            Adnan Khan, Sylvie Amu, Sean P. Saunders, and Padraic G. Fallon


12. Use of Co-Cultures for the Study of Cellular Interactions Influencing B Cell Regulatory Functions

            Francesca Mion, Federica Benvenuti, Carlo Pucillo, and Gaetano Vitale


Part IV: Study of Regulatory B Cells in Pathological Settings


13. Regulatory B Cells in Experimental Mouse Models of Arthritis

            Elizabeth C. Rosser and Claudia Mauri


14. Regulatory B Cells in Mouse Models of Systemic Lupus Erythematosus (SLE)

            Takashi Matsushita


15. Regulatory B Cells in Allergic Airways Disease and Asthma

            Prabitha Natarajan, Linda A. Guernsey, and Craig M. Schramm


16. Regulatory B Cells in Mouse Models of Intestinal Inflammation

            Atsushi Nishida, Cindy W. Lau, Emiko Mizoguchi, and Atsushi Mizoguchi


17. Regulatory B Cells in Experimental Autoimmune Encephalomyelitis (EAE)

            Avijit Ray and Sreemanti Basu


18. Regulatory B Cells, Helminths, and Multiple Sclerosis

            Jorge Correale and Tomas Rivero Equiza


19. Generation and Identification of Tumor-Evoked Regulatory B Cells

            Arya Biragyn, Catalina Lee-Chang, and Monica Bodogai

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