Overview
- Editors:
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Ivana Vancurova
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Department of Biological Sciences, St. John's University, New York, USA
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
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Table of contents (31 protocols)
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Detection of Released Cytokines by Immunoassays
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- Haichao Wang, Lin Zhao, Jianhua Li, Shu Zhu, Maggie Yeung
Pages 13-25
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- Camilla Heldbjerg Drabe, Thomas Blauenfeldt, Morten Ruhwald
Pages 27-37
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- Benoît Liberelle, Charles Fortier, Gregory De Crescenzo
Pages 39-47
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- Michael Lee, Abdoullatif Baraket, Nadia Zine, Miguel Zabala, Francesca Campabadal, Raffaele Caruso et al.
Pages 49-64
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- Gemma Moncunill, Joseph J. Campo, Carlota Dobaño
Pages 65-86
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Cytokine Bioassays
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- Hardik Patel, Dennis Davidson
Pages 99-106
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- Dennis Davidson, Hardik Patel
Pages 107-113
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- Ved P. Sharma, Brian T. Beaty, Dianne Cox, John S. Condeelis, Robert J. Eddy
Pages 115-123
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- Michael Cammer, Dianne Cox
Pages 125-135
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- Lokesh Sharma, Wenjun Wu, Sanjay L. Dholakiya, Samir Gorasiya, Jiao Wu, Ravikumar Sitapara et al.
Pages 137-145
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- Christopher J. Kirk, Saul R. Powell, Edmund J. Miller
Pages 147-162
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- Veronika Miskolci, Désirée Spiering, Dianne Cox, Louis Hodgson
Pages 173-184
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- Mahmud Arif Pavel, Clarissa Lam, Parul Kashyap, Zahra Salehi-Najafabadi, Gurpreet Singh, Yong Yu
Pages 185-192
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- Clarissa Lam, Mahmud Arif Pavel, Parul Kashyap, Zahra Salehi-Najafabadi, Victoria Valentino, Yong Yu
Pages 193-200
About this book
Cytokines are pleiotropic regulatory proteins involved in essentially all biological processes and associated with a wide variety of diseases, including inflammatory disorders as well as many types of cancer and leukemia. Knowledge about the quantitative and qualitative nature of cytokine production is critical in the understanding of normal and pathological processes. The cytokine detection in biological and clinical samples faces many challenges including their low abundance, the need to distinguish between active and latent cytokine forms, and the need to measure multiple cytokines in a single assay. This volume will provide a comprehensive collection of classic and cutting-edge methodologies that are currently used to analyze and quantify cytokines and their biological activities in complex biological and clinical samples. The chapters are divided into four main categories. The first group focuses on the immunodetection of released cytokines in tissue culture supernatants, plasma, serum and whole blood samples by immunoassays. These immunoassays measure the total concentrations of released cytokines regardless of their biological activity and include ELISA, flow cytometry, ELISPOT and the antibody-based proximity ligation. The second group will focus on the analysis of biologically active cytokines by bioassays using neutralizing antibodies, chemotaxis assay, cytokine-induced cell degranulation assay, cell proliferation and differentiation, cytokine-induced cytokine production and the radioreceptor cytokine assay. The third group focuses on the analysis of intracellular cytokines by flow cytometry, western blotting and fluorescence and confocal microscopy. In addition, this category includes protocols for quantitative analysis of cytokine gene expression by real time RT-PCR and analysis of the cytokine promoter occupancy by chromatin immunoprecipitation. The fourth group focuses on the recently developed multiplex arrays that can measure multiplecytokines in the same sample at the same time. This group includes quantification of multiple cytokines using cytometric bead arrays, ELISPOT assays, proteomics cytokine evaluation, multiplexed proximity ligation assays for high-throughput cytokine analysis and finally, cytokine gene expression analysis by gene arrays. The protocols will be written by experienced basic and clinical researchers with hands-on knowledge of the described protocols. By covering a broad variety of methods used in cytokine detection and analysis, this book will be of interest not only to biochemists, molecular biologists and immunologists but also to physician-scientists working in the field of cytokine research.
Editors and Affiliations
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Department of Biological Sciences, St. John's University, New York, USA
Ivana Vancurova
