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Biomedical Sciences - Immunology | DNA Vaccines - Methods and Protocols

DNA Vaccines

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1143

Rinaldi, Monica, Fioretti, Daniela, Iurescia, Sandra (Eds.)

3rd ed. 2014, XI, 308 p. 43 illus., 32 illus. in color.

A product of Humana Press
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  • Includes cutting-edge methods and protocols involving DNA vaccine applications
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

DNA Vaccines: Methods and Protocols, Third Edition explores innovative approaches and technologies used to design, deliver, and enhance the efficacy of DNA vaccines.  Featuring applications which should be of great value in moving vaccines from research to clinic, this detailed volume includes sections on DNA vaccine design and enhancement, delivery systems, production, purification, and quality, as well as chapters on new vaccine applications.  Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, DNA Vaccines: Methods and Protocols, Third Edition serves the important role of further documenting the potential of the DNA vaccination as a platform technology for treatment and prevention of human disease.

Content Level » Professional/practitioner

Keywords » DNA vaccination - Gene vaccines - Non-viral gene therapy - Production and purification - Vaccine design and development

Related subjects » Biotechnology - Immunology - Medical Microbiology

Table of contents 

Part I: DNA Vaccine Design and Enhancement


1. A Blueprint for DNA Vaccines Design

            Sandra Iurescia, Daniela Fioretti, and Monica Rinaldi


2. Enhancement of Plasmid-Mediated Transgene Expression

            Daniela Fioretti, Sandra Iurescia, and Monica Rinaldi


3. Strategies for Improving DNA Vaccines Performance

            Sandra Iurescia, Daniela Fioretti, and Monica Rinaldi


4. Enhancement of DNA Vaccine Efficacy by Intracellular Targeting Strategies

            Elisabete Borges Freitas, Ana Margarida Henriques, Miguel Fevereiro, Duarte Miguel Prazeres, and Gabriel Amaro Monteiro


5. Progresses in DNA-Based Heterologous Prime-Boost Immunization Strategies

            Ronald J. Jackson, David B. Boyle, and Charani Ranasinghe


6. Development of Antibiotic-Free Selection System for Safer DNA Vaccination

            Jeremy M. Luke, Aaron E. Carnes, and James A. Williams


Part II: Delivery System


7. Electroporation-Based DNA Delivery Technology: Methods for Gene Electrotransfer to Skin

            Anita Gothelf and Julie Gehl


8. DNA Vaccination in Skin Enhanced by Electroporation

            Kate E. Broderick, Amir S. Khan, and Niranjan S. Sardesai


9. Intradermal Vaccination by DNA Tattooing

            Joost H. van den Berg, Koen Oosterhuis, Ton N.M. Schumacher, John B.A.G. Haanen, and Adriaan D. Bins


10. Microneedle Applications for DNA Vaccine Delivery to the Skin

            Hae-yong Seok, Hyemee Suh, Sunghyun Baek, and Yeu-Chun Kim


11. Multivalent DNA-Based Vectors for DNA Vaccines Delivery

            Young Hoon Roh, Kwang Lee, Jessica Jane Ye, and Dan Luo


12. Superparamagnetic Nanoparticles Delivery of DNA Vaccine

            Fatin Nawwab Al-Deen, Cordelia Selomulya, Charles Ma, and Ross L. Coppel


Part III: Production, Purification, and Quality


13. Plasmid Fermentation Process for DNA Immunization Applications

            Aaron E. Carnes and James A. Williams


14. Pharmaceutical Grade Large Scale Plasmid DNA Manufacturing Process

            Marco Schmeer and Martin Schleef


Part IV: New Vaccine Applications


15. Protective and Therapeutic DNA Vaccination Against Allergic Diseases

            Almedina Isakovic, Richard Weiss, Josef Thalhamer, and Sandra Scheiblhofer


16. Immunotherapy for Alzheimer’s Disease: DNA- and Protein-Based Epitope Vaccines

            Hayk  Davtyan, Irina Petrushina, and Anahit Ghochikyan


17. Tetravalent DNA Vaccine Product as a Vaccine Candidate Against Dengue

            Kevin R. Porter, Nimfa Teneza-Mora, and Kanakatte Raviprakash


18. DNA Vaccination as a Treatment for Chronic Kidney Disease

            Yuan Min Wang and Stephen I. Alexander

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