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  • Book
  • © 2004

Cell Cycle Control and Dysregulation Protocols

Editors:

  1. Antonio Giordano

    1. Sbarro Institute for Cancer Research and Molecular Medicine, Center for Biotechnology, College of Science and Technology, Temple University, Philadelphia

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    You can also search for this editor in PubMed   Google Scholar

  2. Gaetano Romano

    1. Department of Neurosurgery, Thomas Jefferson University, Philadelphia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 285)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xiii
    PDF

  2. Analysis of Cyclins and Cyclin-Dependent Kinases

    1. Front Matter

      Pages 1-1
      PDF

    3. In Situ Immunofluorescence Analysis

      • Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan et al.
      Pages 23-28

    4. In Situ Immunofluorescence Analysis

      • Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan et al.
      Pages 29-31

    5. Immunofluorescence Analysis Using Epitope-Tagged Proteins

      • Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan et al.
      Pages 33-36

    6. Analysis of In Vivo Gene Expression Using Epitope-Tagged Proteins

      • Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan et al.
      Pages 37-40

    7. Chromatin Immunoprecipitation

      • Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan et al.
      Pages 41-44

    8. Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expression

      • Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan et al.
      Pages 45-55

    9. Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expression

      • Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan et al.
      Pages 57-62

    10. Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expression

      • Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan et al.
      Pages 63-67

    11. Assays for Cyclin-Dependent Kinase Inhibitors

      • Adrian M. Senderowicz
      Pages 69-78

    12. Protein Degradation Via the Proteosome

      • Henry Hoff, Hong Zhang, Christian Sell
      Pages 79-92

  3. Analysis of the Factors Involved in Cell Cycle Deregulation

    1. Front Matter

      Pages 93-93
      PDF

    2. The Transformed Phenotype

      • Henry Hoff, Barbara Belletti, Hong Zhang, Christian Sell
      Pages 95-104

    3. A Morphologic Approach to Detect Apoptosis Based on Electron Microscopy

      • Martyn K. White, Caterina Cinti
      Pages 105-111

    4. Detection of Apoptotic Deoxyribonucleic Acid Break by In Situ Nick Translation

      • Carmela Trimarchi, Dario La Sala, Alessandra Zamparelli, Caterina Cinti
      Pages 113-118

  4. Cellular Response to Deoxyribonucleic Acid Damage

    1. Front Matter

      Pages 119-119
      PDF

    2. Induction of Deoxyribonucleic Acid Damage by Alkylating Agents

      • Salvatore Cortellino, David P. Turner, Domenico Albino, Alfonso Bellacosa
      Pages 121-125

    3. Induction of Deoxyribonucleic Acid Damage by γ Irradiation

      • Salvatore Cortellino, David P. Turner, Alfonso Bellacosa
      Pages 127-132
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About this book

Cell Cycle Control and Dysregulation Protocols focuses on emerging methodologies for studying the cell cycle, kinases, and kinase inhibitors. It addresses the issue of gene expression in vivo and in vitro, the analysis of cyclin-dependent kinase inhibitors, protein degradation mediated by the proteosome, the analysis of the transformed cell phenotype, and innovative techniques to detect apoptosis. Because there are already many manuals and protocols available, along with commercial kits and reagents, a variety of the more common techniques have not been included in our book. The protocols described, based on rather sophisticated techniques for in vivo and in vitro studies, consist of molecular biology, biochemistry, and various types of immunoassays. Indeed, the authors have successfully accomplished an arduous task by presenting several topics in the simplest possible manner. We are confident that Cell Cycle Control and Dysregulation Protocols will facilitate and optimize the work of practical scientists involved in researching the cell cycle. We greatly acknowledge the extraordinary contribution of the authors in writing this book.
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Reviews

"State-of-the-art and highly practical...offers pathbreaking researchers powerful tools for probing the cell cycle regulation across a wide variety of pathologies." - Tumori
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Editors and Affiliations

  • Sbarro Institute for Cancer Research and Molecular Medicine, Center for Biotechnology, College of Science and Technology, Temple University, Philadelphia

    Antonio Giordano

  • Department of Neurosurgery, Thomas Jefferson University, Philadelphia

    Gaetano Romano

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Bibliographic Information

  • Book Title: Cell Cycle Control and Dysregulation Protocols

  • Editors: Antonio Giordano, Gaetano Romano

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592598226

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • Hardcover ISBN: 978-0-89603-949-0Published: 19 July 2004

  • Softcover ISBN: 978-1-61737-270-4Published: 10 November 2010

  • eBook ISBN: 978-1-59259-822-9Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 185

  • Topics: Immunology

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Buy it now

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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